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MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus

Schematic summary of carbohydrates utilization by ABC transporters in S. mutans (A), S. pneumoniae (B) and S. suis (C).Above each ABC complexes is a list of known or putative carbohydrates transported by each ABC transporter. Bidirectional arrow means the MsmK and MalK ATPases can energize permeases interactively. Unidirectional arrows mean the degradation of pullulan and glycogen by pullulanases SpuA or ApuA.
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pone.0130792.g007: Schematic summary of carbohydrates utilization by ABC transporters in S. mutans (A), S. pneumoniae (B) and S. suis (C).Above each ABC complexes is a list of known or putative carbohydrates transported by each ABC transporter. Bidirectional arrow means the MsmK and MalK ATPases can energize permeases interactively. Unidirectional arrows mean the degradation of pullulan and glycogen by pullulanases SpuA or ApuA.

Mentions: It is well known that carbohydrate utilization is essential for the growth and pathogenesis of many pathogenic bacteria. As necessary nutrients, most carbohydrates must be transported into bacterial cells, and then used for different biological processes. There are mainly two carbohydrate transport systems in bacteria: the PTS system and ABC transporters. As an ABC transporter, two permease domains and two ATPase subunits are essential structural components. The two permeases form an integral membrane channel, and the two ATPase subunits energize the transport though ATP hydrolysis. Interestingly, some ABC transporters possess their own ATPase, while others share a common ATPase. For example, MsmK functions as a shared ATPase for three carbohydrate ABC transporters RafEFG, SatABC and MalXCD in S. penumoniae (Fig 7) [32,35]. S. suis is an important emerging zoonotic bacterium causing increasing dead infections in pigs and humans worldwide, especially in Southeast Asia and North Europe. Here, we identified MsmK as a shared ATPase energizing the transport of raffinose, melibiose, maltotetraose, glycogen and maltotriose in S. suis.


MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Schematic summary of carbohydrates utilization by ABC transporters in S. mutans (A), S. pneumoniae (B) and S. suis (C).Above each ABC complexes is a list of known or putative carbohydrates transported by each ABC transporter. Bidirectional arrow means the MsmK and MalK ATPases can energize permeases interactively. Unidirectional arrows mean the degradation of pullulan and glycogen by pullulanases SpuA or ApuA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519317&req=5

pone.0130792.g007: Schematic summary of carbohydrates utilization by ABC transporters in S. mutans (A), S. pneumoniae (B) and S. suis (C).Above each ABC complexes is a list of known or putative carbohydrates transported by each ABC transporter. Bidirectional arrow means the MsmK and MalK ATPases can energize permeases interactively. Unidirectional arrows mean the degradation of pullulan and glycogen by pullulanases SpuA or ApuA.
Mentions: It is well known that carbohydrate utilization is essential for the growth and pathogenesis of many pathogenic bacteria. As necessary nutrients, most carbohydrates must be transported into bacterial cells, and then used for different biological processes. There are mainly two carbohydrate transport systems in bacteria: the PTS system and ABC transporters. As an ABC transporter, two permease domains and two ATPase subunits are essential structural components. The two permeases form an integral membrane channel, and the two ATPase subunits energize the transport though ATP hydrolysis. Interestingly, some ABC transporters possess their own ATPase, while others share a common ATPase. For example, MsmK functions as a shared ATPase for three carbohydrate ABC transporters RafEFG, SatABC and MalXCD in S. penumoniae (Fig 7) [32,35]. S. suis is an important emerging zoonotic bacterium causing increasing dead infections in pigs and humans worldwide, especially in Southeast Asia and North Europe. Here, we identified MsmK as a shared ATPase energizing the transport of raffinose, melibiose, maltotetraose, glycogen and maltotriose in S. suis.

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus