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MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus

MsmK contributes to streptococcal survival in brain.Mice were received intraperitoneal injection with 3 × 107 CFU of a 1: 1 mix of SC-19 and ΔmsmK strains. At each defined time point, brains of 5 mice were collected and the viable bacteria were counted. The SC-19 and ΔmsmK strains were distinguished by erythromycin added in TSA plates. Solid lines, the means of data of strain SC-19; dotted lines, the means of data of strain ΔmsmK. The means and standard errors of the means are depicted. Statistical significance was tested by a two-tailed Student t test (*, P ≤ 0.05; ***, P ≤ 0.001).
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pone.0130792.g006: MsmK contributes to streptococcal survival in brain.Mice were received intraperitoneal injection with 3 × 107 CFU of a 1: 1 mix of SC-19 and ΔmsmK strains. At each defined time point, brains of 5 mice were collected and the viable bacteria were counted. The SC-19 and ΔmsmK strains were distinguished by erythromycin added in TSA plates. Solid lines, the means of data of strain SC-19; dotted lines, the means of data of strain ΔmsmK. The means and standard errors of the means are depicted. Statistical significance was tested by a two-tailed Student t test (*, P ≤ 0.05; ***, P ≤ 0.001).

Mentions: To study the functions of MsmK in pathogenesis, ΔmsmK and its parental strain SC-19 were equally mixed and inoculated into mice. For meningitis is the most striking feature caused by S. suis and often the basis of a presumptive diagnosis [18], the bacterial loads of two strains in brain were measured at different time points post infection. Data showed that the numbers of the mutant were significantly decreased than that of the wild type strain in brain from the third day (Fig 6). These results indicate that MsmK contributes to in vivo survival and colonization of S. suis in mice.


MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

MsmK contributes to streptococcal survival in brain.Mice were received intraperitoneal injection with 3 × 107 CFU of a 1: 1 mix of SC-19 and ΔmsmK strains. At each defined time point, brains of 5 mice were collected and the viable bacteria were counted. The SC-19 and ΔmsmK strains were distinguished by erythromycin added in TSA plates. Solid lines, the means of data of strain SC-19; dotted lines, the means of data of strain ΔmsmK. The means and standard errors of the means are depicted. Statistical significance was tested by a two-tailed Student t test (*, P ≤ 0.05; ***, P ≤ 0.001).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519317&req=5

pone.0130792.g006: MsmK contributes to streptococcal survival in brain.Mice were received intraperitoneal injection with 3 × 107 CFU of a 1: 1 mix of SC-19 and ΔmsmK strains. At each defined time point, brains of 5 mice were collected and the viable bacteria were counted. The SC-19 and ΔmsmK strains were distinguished by erythromycin added in TSA plates. Solid lines, the means of data of strain SC-19; dotted lines, the means of data of strain ΔmsmK. The means and standard errors of the means are depicted. Statistical significance was tested by a two-tailed Student t test (*, P ≤ 0.05; ***, P ≤ 0.001).
Mentions: To study the functions of MsmK in pathogenesis, ΔmsmK and its parental strain SC-19 were equally mixed and inoculated into mice. For meningitis is the most striking feature caused by S. suis and often the basis of a presumptive diagnosis [18], the bacterial loads of two strains in brain were measured at different time points post infection. Data showed that the numbers of the mutant were significantly decreased than that of the wild type strain in brain from the third day (Fig 6). These results indicate that MsmK contributes to in vivo survival and colonization of S. suis in mice.

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus