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MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus

Deletion of msmK impacts bacterial growth on maltotriose.The SC-19, ΔmsmK and CΔmsmK strains were grown on the CDM containing 1% glucose (A), 1% maltose (B), 1% maltotriose (C) or no sugar (D) as sole carbon source. CDM supplemented with glucose/no sugar served as a positive/negative control respectively in growth assays. Growth was measured by the optical density at 600 nm. Data are means from at least three independent experiments.
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pone.0130792.g004: Deletion of msmK impacts bacterial growth on maltotriose.The SC-19, ΔmsmK and CΔmsmK strains were grown on the CDM containing 1% glucose (A), 1% maltose (B), 1% maltotriose (C) or no sugar (D) as sole carbon source. CDM supplemented with glucose/no sugar served as a positive/negative control respectively in growth assays. Growth was measured by the optical density at 600 nm. Data are means from at least three independent experiments.

Mentions: To detect if MsmK contributes to the utilization of other carbohydrates, S. suis strains were cultured in CDM supplemented with glucose, maltose or maltotriose as sole carbon resource (Fig 4A–4C). CDM supplemented with no sugar served as negative control in growth assays (Fig 4D). Interestingly, the mutant displayed growth defect in the CDM containing glucose compared with the parental strain according to the OD values (Fig 4A), but there was no significant difference between the two strains when measuring the numbers of living bacterium at stable stage (S3 Fig). The same phenomenon was observed on the mutant when cultured in maltose (Fig 4B). Unlikely in glucose and maltose, the growth of ΔmsmK was largely inhibited when using maltotriose as sole carbon source (Fig 4C).


MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Deletion of msmK impacts bacterial growth on maltotriose.The SC-19, ΔmsmK and CΔmsmK strains were grown on the CDM containing 1% glucose (A), 1% maltose (B), 1% maltotriose (C) or no sugar (D) as sole carbon source. CDM supplemented with glucose/no sugar served as a positive/negative control respectively in growth assays. Growth was measured by the optical density at 600 nm. Data are means from at least three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519317&req=5

pone.0130792.g004: Deletion of msmK impacts bacterial growth on maltotriose.The SC-19, ΔmsmK and CΔmsmK strains were grown on the CDM containing 1% glucose (A), 1% maltose (B), 1% maltotriose (C) or no sugar (D) as sole carbon source. CDM supplemented with glucose/no sugar served as a positive/negative control respectively in growth assays. Growth was measured by the optical density at 600 nm. Data are means from at least three independent experiments.
Mentions: To detect if MsmK contributes to the utilization of other carbohydrates, S. suis strains were cultured in CDM supplemented with glucose, maltose or maltotriose as sole carbon resource (Fig 4A–4C). CDM supplemented with no sugar served as negative control in growth assays (Fig 4D). Interestingly, the mutant displayed growth defect in the CDM containing glucose compared with the parental strain according to the OD values (Fig 4A), but there was no significant difference between the two strains when measuring the numbers of living bacterium at stable stage (S3 Fig). The same phenomenon was observed on the mutant when cultured in maltose (Fig 4B). Unlikely in glucose and maltose, the growth of ΔmsmK was largely inhibited when using maltotriose as sole carbon source (Fig 4C).

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus