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MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus

MsmK is required for growth in raffinose, melibiose, glycogen and maltotetraose.The SC-19, ΔmsmK and CΔmsmK strains were grown in CDM containing 1% raffinose (A), 1% melibiose (B), 1% glycogen (C) or 1% maltotetraose (D) as sole carbon source. Growth was measured by the optical density at 600 nm. Data are presented as means from at least three independent experiments.
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pone.0130792.g003: MsmK is required for growth in raffinose, melibiose, glycogen and maltotetraose.The SC-19, ΔmsmK and CΔmsmK strains were grown in CDM containing 1% raffinose (A), 1% melibiose (B), 1% glycogen (C) or 1% maltotetraose (D) as sole carbon source. Growth was measured by the optical density at 600 nm. Data are presented as means from at least three independent experiments.

Mentions: Additionally, deletion of msmK resulted in dramatically growth inhibition compared to the wild type strain when cultured in CDM containing raffinose, melibiose or glycogen (Fig 3A–3C) as sole carbon source. Since MsmK energizes the MalXCD transporters to transport maltodextrins in S. pneumoniae [23,32], ΔmsmK was also tested for growth in CDM with maltotetraose as sole carbohydrate. The results showed that the mutant was unable to grow in this medium (Fig 3D). CΔmsmK completely or partially restored the growth defect in all growth assays. These results demonstrate that MsmK is responsible for utilization of raffinose, melibiose, glycogen and malitrotetraose in S. suis, which also imply that MsmK energizes the MsmEFG and MalXCD transporters in S. suis.


MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

MsmK is required for growth in raffinose, melibiose, glycogen and maltotetraose.The SC-19, ΔmsmK and CΔmsmK strains were grown in CDM containing 1% raffinose (A), 1% melibiose (B), 1% glycogen (C) or 1% maltotetraose (D) as sole carbon source. Growth was measured by the optical density at 600 nm. Data are presented as means from at least three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519317&req=5

pone.0130792.g003: MsmK is required for growth in raffinose, melibiose, glycogen and maltotetraose.The SC-19, ΔmsmK and CΔmsmK strains were grown in CDM containing 1% raffinose (A), 1% melibiose (B), 1% glycogen (C) or 1% maltotetraose (D) as sole carbon source. Growth was measured by the optical density at 600 nm. Data are presented as means from at least three independent experiments.
Mentions: Additionally, deletion of msmK resulted in dramatically growth inhibition compared to the wild type strain when cultured in CDM containing raffinose, melibiose or glycogen (Fig 3A–3C) as sole carbon source. Since MsmK energizes the MalXCD transporters to transport maltodextrins in S. pneumoniae [23,32], ΔmsmK was also tested for growth in CDM with maltotetraose as sole carbohydrate. The results showed that the mutant was unable to grow in this medium (Fig 3D). CΔmsmK completely or partially restored the growth defect in all growth assays. These results demonstrate that MsmK is responsible for utilization of raffinose, melibiose, glycogen and malitrotetraose in S. suis, which also imply that MsmK energizes the MsmEFG and MalXCD transporters in S. suis.

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus