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MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus

Schematic presentation of the msm and mal loci encoding relevant carbohydrate ABC transporters in selected streptococci.The genes within the loci encoding components of the S. suis MsmEFG (A), S. mutans MsmEFGK (B), S. pneuminiae MsmEFG (C), S. suis MalXCD (D), S. mutans MalXFGK (E) and S. pneumoniae MalXCD (F) are represented. Arrows indicate the direction of transcription. Gray arrows, genes encoding the ATPase of ABC transporters; spotted arrows, genes encoding solute binding proteins; black arrows, genes encoding permeases of ABC transporters; blank arrows, other genes adjacent.
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pone.0130792.g001: Schematic presentation of the msm and mal loci encoding relevant carbohydrate ABC transporters in selected streptococci.The genes within the loci encoding components of the S. suis MsmEFG (A), S. mutans MsmEFGK (B), S. pneuminiae MsmEFG (C), S. suis MalXCD (D), S. mutans MalXFGK (E) and S. pneumoniae MalXCD (F) are represented. Arrows indicate the direction of transcription. Gray arrows, genes encoding the ATPase of ABC transporters; spotted arrows, genes encoding solute binding proteins; black arrows, genes encoding permeases of ABC transporters; blank arrows, other genes adjacent.

Mentions: The CUT1, CUT2 and peptide/opine/nickel uptake transporter (PepT) subfamily are three subfamilies classified as ABC transporters [51]. The genome of SC84 includes two regions encoding the CUT1 subfamily of ABC transporters: MsmEFG and MalXCD (Fig 1A and 1D), both of which consist of a solute binding protein (MsmE and MalX) and two membrane proteins (MsmF/MsmG and MalC/MalD).


MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Tan MF, Gao T, Liu WQ, Zhang CY, Yang X, Zhu JW, Teng MY, Li L, Zhou R - PLoS ONE (2015)

Schematic presentation of the msm and mal loci encoding relevant carbohydrate ABC transporters in selected streptococci.The genes within the loci encoding components of the S. suis MsmEFG (A), S. mutans MsmEFGK (B), S. pneuminiae MsmEFG (C), S. suis MalXCD (D), S. mutans MalXFGK (E) and S. pneumoniae MalXCD (F) are represented. Arrows indicate the direction of transcription. Gray arrows, genes encoding the ATPase of ABC transporters; spotted arrows, genes encoding solute binding proteins; black arrows, genes encoding permeases of ABC transporters; blank arrows, other genes adjacent.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519317&req=5

pone.0130792.g001: Schematic presentation of the msm and mal loci encoding relevant carbohydrate ABC transporters in selected streptococci.The genes within the loci encoding components of the S. suis MsmEFG (A), S. mutans MsmEFGK (B), S. pneuminiae MsmEFG (C), S. suis MalXCD (D), S. mutans MalXFGK (E) and S. pneumoniae MalXCD (F) are represented. Arrows indicate the direction of transcription. Gray arrows, genes encoding the ATPase of ABC transporters; spotted arrows, genes encoding solute binding proteins; black arrows, genes encoding permeases of ABC transporters; blank arrows, other genes adjacent.
Mentions: The CUT1, CUT2 and peptide/opine/nickel uptake transporter (PepT) subfamily are three subfamilies classified as ABC transporters [51]. The genome of SC84 includes two regions encoding the CUT1 subfamily of ABC transporters: MsmEFG and MalXCD (Fig 1A and 1D), both of which consist of a solute binding protein (MsmE and MalX) and two membrane proteins (MsmF/MsmG and MalC/MalD).

Bottom Line: Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose.In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains.Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

No MeSH data available.


Related in: MedlinePlus