Limits...
BAI1-Associated Protein 2-Like 1 (BAIAP2L1) Is a Potential Biomarker in Ovarian Cancer.

Chao A, Tsai CL, Jung SM, Chuang WC, Kao C, Hsu A, Chen SH, Lin CY, Lee YC, Lee YS, Wang TH, Wang HS, Lai CH - PLoS ONE (2015)

Bottom Line: Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration.BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma.Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.

ABSTRACT
Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration. BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma. In this study, we report the first comprehensive study of BAIAP2L1 upregulation in human ovarian cancer. Upregulation of BAIAP2L1 in ovarian tumors was first found during RNA screening and confirmed by immunohistochemical studies on ovarian cancers and other cancer types. Significant upregulation of BAIAP2L1 in ovarian cancer was validated by analyzing multiple independent cohorts in publicly available data sets. Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors. Functional assays in ovarian cancer cells revealed that BAIAP2L1 is involved in promoting cell proliferation and avoiding apoptosis. In conclusion, results of this study not only indicate that BAIAP2L1 can be used as a biomarker for human ovarian cancer but also reveal its role in cancer biology. Further elucidation of the role of BAIAP2L1 in context of the insulin receptor signaling pathways of cancer cells is warranted for developing cancer therapeutics by targeting cancer-specific metabolism.

No MeSH data available.


Related in: MedlinePlus

Knockdown of endogenous BAIAP2L1 inhibits cell proliferation and enhances apoptosis induced by UV and cisplatin.BAIAP2L1 in two ovarian cancer cell lines (MDAH2774 and SKOV3) were knockdowned with si-RNA (A) and analyzed with (B) BrdU incorportation assay and (C) MTT assay. (D) BAIAP2L1 knockdown in SKOV3 cells increased cleavage of caspase 3 and PARP, which were indicators of apoptosis. The cells were irradiated with UV (100 J/M2) or treated with 10 μM cisplatin for 24 h to induce apoptosis. To clearly visualize the differential intensities of cleaved PARP bands, two exposure times were used during chemiluminescence: long (60 sec) and short (10 sec). The actin level was used to normalize the amount of input protein. (E) Quantitative analyses of cleaved caspase 3 and PARP. Results shown are the mean ± standard error from three independent experiments. Asterisks denote statistical significance (p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4519316&req=5

pone.0133081.g006: Knockdown of endogenous BAIAP2L1 inhibits cell proliferation and enhances apoptosis induced by UV and cisplatin.BAIAP2L1 in two ovarian cancer cell lines (MDAH2774 and SKOV3) were knockdowned with si-RNA (A) and analyzed with (B) BrdU incorportation assay and (C) MTT assay. (D) BAIAP2L1 knockdown in SKOV3 cells increased cleavage of caspase 3 and PARP, which were indicators of apoptosis. The cells were irradiated with UV (100 J/M2) or treated with 10 μM cisplatin for 24 h to induce apoptosis. To clearly visualize the differential intensities of cleaved PARP bands, two exposure times were used during chemiluminescence: long (60 sec) and short (10 sec). The actin level was used to normalize the amount of input protein. (E) Quantitative analyses of cleaved caspase 3 and PARP. Results shown are the mean ± standard error from three independent experiments. Asterisks denote statistical significance (p < 0.05).

Mentions: Since BAIAP2L1 has been shown to promote cell proliferation in hepatocellular carcinoma (HCC) [22], we also tested the role of BAIAP2L1 in cell growth of ovarian cancer cells. Knockdown of BAIAP2L1 with (Fig 6A) siRNA technology inhibited cell growth in multiple ovarian cancer cell lines, shown in results of BrDU incorporation assays (Fig 6B) and MTT assays (Fig 6C).


BAI1-Associated Protein 2-Like 1 (BAIAP2L1) Is a Potential Biomarker in Ovarian Cancer.

Chao A, Tsai CL, Jung SM, Chuang WC, Kao C, Hsu A, Chen SH, Lin CY, Lee YC, Lee YS, Wang TH, Wang HS, Lai CH - PLoS ONE (2015)

Knockdown of endogenous BAIAP2L1 inhibits cell proliferation and enhances apoptosis induced by UV and cisplatin.BAIAP2L1 in two ovarian cancer cell lines (MDAH2774 and SKOV3) were knockdowned with si-RNA (A) and analyzed with (B) BrdU incorportation assay and (C) MTT assay. (D) BAIAP2L1 knockdown in SKOV3 cells increased cleavage of caspase 3 and PARP, which were indicators of apoptosis. The cells were irradiated with UV (100 J/M2) or treated with 10 μM cisplatin for 24 h to induce apoptosis. To clearly visualize the differential intensities of cleaved PARP bands, two exposure times were used during chemiluminescence: long (60 sec) and short (10 sec). The actin level was used to normalize the amount of input protein. (E) Quantitative analyses of cleaved caspase 3 and PARP. Results shown are the mean ± standard error from three independent experiments. Asterisks denote statistical significance (p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519316&req=5

pone.0133081.g006: Knockdown of endogenous BAIAP2L1 inhibits cell proliferation and enhances apoptosis induced by UV and cisplatin.BAIAP2L1 in two ovarian cancer cell lines (MDAH2774 and SKOV3) were knockdowned with si-RNA (A) and analyzed with (B) BrdU incorportation assay and (C) MTT assay. (D) BAIAP2L1 knockdown in SKOV3 cells increased cleavage of caspase 3 and PARP, which were indicators of apoptosis. The cells were irradiated with UV (100 J/M2) or treated with 10 μM cisplatin for 24 h to induce apoptosis. To clearly visualize the differential intensities of cleaved PARP bands, two exposure times were used during chemiluminescence: long (60 sec) and short (10 sec). The actin level was used to normalize the amount of input protein. (E) Quantitative analyses of cleaved caspase 3 and PARP. Results shown are the mean ± standard error from three independent experiments. Asterisks denote statistical significance (p < 0.05).
Mentions: Since BAIAP2L1 has been shown to promote cell proliferation in hepatocellular carcinoma (HCC) [22], we also tested the role of BAIAP2L1 in cell growth of ovarian cancer cells. Knockdown of BAIAP2L1 with (Fig 6A) siRNA technology inhibited cell growth in multiple ovarian cancer cell lines, shown in results of BrDU incorporation assays (Fig 6B) and MTT assays (Fig 6C).

Bottom Line: Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration.BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma.Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.

ABSTRACT
Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration. BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma. In this study, we report the first comprehensive study of BAIAP2L1 upregulation in human ovarian cancer. Upregulation of BAIAP2L1 in ovarian tumors was first found during RNA screening and confirmed by immunohistochemical studies on ovarian cancers and other cancer types. Significant upregulation of BAIAP2L1 in ovarian cancer was validated by analyzing multiple independent cohorts in publicly available data sets. Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors. Functional assays in ovarian cancer cells revealed that BAIAP2L1 is involved in promoting cell proliferation and avoiding apoptosis. In conclusion, results of this study not only indicate that BAIAP2L1 can be used as a biomarker for human ovarian cancer but also reveal its role in cancer biology. Further elucidation of the role of BAIAP2L1 in context of the insulin receptor signaling pathways of cancer cells is warranted for developing cancer therapeutics by targeting cancer-specific metabolism.

No MeSH data available.


Related in: MedlinePlus