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BAI1-Associated Protein 2-Like 1 (BAIAP2L1) Is a Potential Biomarker in Ovarian Cancer.

Chao A, Tsai CL, Jung SM, Chuang WC, Kao C, Hsu A, Chen SH, Lin CY, Lee YC, Lee YS, Wang TH, Wang HS, Lai CH - PLoS ONE (2015)

Bottom Line: Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration.BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma.Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.

ABSTRACT
Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration. BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma. In this study, we report the first comprehensive study of BAIAP2L1 upregulation in human ovarian cancer. Upregulation of BAIAP2L1 in ovarian tumors was first found during RNA screening and confirmed by immunohistochemical studies on ovarian cancers and other cancer types. Significant upregulation of BAIAP2L1 in ovarian cancer was validated by analyzing multiple independent cohorts in publicly available data sets. Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors. Functional assays in ovarian cancer cells revealed that BAIAP2L1 is involved in promoting cell proliferation and avoiding apoptosis. In conclusion, results of this study not only indicate that BAIAP2L1 can be used as a biomarker for human ovarian cancer but also reveal its role in cancer biology. Further elucidation of the role of BAIAP2L1 in context of the insulin receptor signaling pathways of cancer cells is warranted for developing cancer therapeutics by targeting cancer-specific metabolism.

No MeSH data available.


Related in: MedlinePlus

BAIAP2L1 histoscores in different subtypes of ovarian cancer.Immunohistochemical studies were performed on ovarian cancer tissues (n = 193) and normal controls (n = 20). Upper panel indicates the immunohistochemical staining intensity (0 = negative, 1 = weak, 2 = moderate, 3 = strong). The overall immunohistochemical score (histoscore) was the percentage of positive cells multiplied by its staining intensity, and ranged from 0 to 300 (100% multiplied by 3).
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pone.0133081.g003: BAIAP2L1 histoscores in different subtypes of ovarian cancer.Immunohistochemical studies were performed on ovarian cancer tissues (n = 193) and normal controls (n = 20). Upper panel indicates the immunohistochemical staining intensity (0 = negative, 1 = weak, 2 = moderate, 3 = strong). The overall immunohistochemical score (histoscore) was the percentage of positive cells multiplied by its staining intensity, and ranged from 0 to 300 (100% multiplied by 3).

Mentions: Protein levels of BAIAP2L1 in 193 ovarian cancer tissues, containing serous, endometrioid, clear cell, and mucinous cell types were analyzed (Fig 3). Expression levels of BAIAP2L1 were not different among various cell types of ovarian cancer, stage and grade of each histologic subtype, but each type of ovarian cancer expressed significantly higher BAIAP2L1 than normal ovarian tissues (Fig 3). Expression of BAIAP2L1 was further analyzed in 14 paired samples of primary ovarian cancers and their corresponding metastatic sites (S3 Tables). The metastatic sites include omentum (n = 7), lymph node (n = 3), parametrium (n = 1), small bowel (n = 1), cecum (n = 1), and brain (n = 1). Twelve of the 14 pairs had higher BAIAP2L1 expressions in the metastatic sites than in primary tumors (p < 0.05) (Fig 4).


BAI1-Associated Protein 2-Like 1 (BAIAP2L1) Is a Potential Biomarker in Ovarian Cancer.

Chao A, Tsai CL, Jung SM, Chuang WC, Kao C, Hsu A, Chen SH, Lin CY, Lee YC, Lee YS, Wang TH, Wang HS, Lai CH - PLoS ONE (2015)

BAIAP2L1 histoscores in different subtypes of ovarian cancer.Immunohistochemical studies were performed on ovarian cancer tissues (n = 193) and normal controls (n = 20). Upper panel indicates the immunohistochemical staining intensity (0 = negative, 1 = weak, 2 = moderate, 3 = strong). The overall immunohistochemical score (histoscore) was the percentage of positive cells multiplied by its staining intensity, and ranged from 0 to 300 (100% multiplied by 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519316&req=5

pone.0133081.g003: BAIAP2L1 histoscores in different subtypes of ovarian cancer.Immunohistochemical studies were performed on ovarian cancer tissues (n = 193) and normal controls (n = 20). Upper panel indicates the immunohistochemical staining intensity (0 = negative, 1 = weak, 2 = moderate, 3 = strong). The overall immunohistochemical score (histoscore) was the percentage of positive cells multiplied by its staining intensity, and ranged from 0 to 300 (100% multiplied by 3).
Mentions: Protein levels of BAIAP2L1 in 193 ovarian cancer tissues, containing serous, endometrioid, clear cell, and mucinous cell types were analyzed (Fig 3). Expression levels of BAIAP2L1 were not different among various cell types of ovarian cancer, stage and grade of each histologic subtype, but each type of ovarian cancer expressed significantly higher BAIAP2L1 than normal ovarian tissues (Fig 3). Expression of BAIAP2L1 was further analyzed in 14 paired samples of primary ovarian cancers and their corresponding metastatic sites (S3 Tables). The metastatic sites include omentum (n = 7), lymph node (n = 3), parametrium (n = 1), small bowel (n = 1), cecum (n = 1), and brain (n = 1). Twelve of the 14 pairs had higher BAIAP2L1 expressions in the metastatic sites than in primary tumors (p < 0.05) (Fig 4).

Bottom Line: Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration.BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma.Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.

ABSTRACT
Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration. BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma. In this study, we report the first comprehensive study of BAIAP2L1 upregulation in human ovarian cancer. Upregulation of BAIAP2L1 in ovarian tumors was first found during RNA screening and confirmed by immunohistochemical studies on ovarian cancers and other cancer types. Significant upregulation of BAIAP2L1 in ovarian cancer was validated by analyzing multiple independent cohorts in publicly available data sets. Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors. Functional assays in ovarian cancer cells revealed that BAIAP2L1 is involved in promoting cell proliferation and avoiding apoptosis. In conclusion, results of this study not only indicate that BAIAP2L1 can be used as a biomarker for human ovarian cancer but also reveal its role in cancer biology. Further elucidation of the role of BAIAP2L1 in context of the insulin receptor signaling pathways of cancer cells is warranted for developing cancer therapeutics by targeting cancer-specific metabolism.

No MeSH data available.


Related in: MedlinePlus