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BAI1-Associated Protein 2-Like 1 (BAIAP2L1) Is a Potential Biomarker in Ovarian Cancer.

Chao A, Tsai CL, Jung SM, Chuang WC, Kao C, Hsu A, Chen SH, Lin CY, Lee YC, Lee YS, Wang TH, Wang HS, Lai CH - PLoS ONE (2015)

Bottom Line: Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration.BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma.Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.

ABSTRACT
Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration. BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma. In this study, we report the first comprehensive study of BAIAP2L1 upregulation in human ovarian cancer. Upregulation of BAIAP2L1 in ovarian tumors was first found during RNA screening and confirmed by immunohistochemical studies on ovarian cancers and other cancer types. Significant upregulation of BAIAP2L1 in ovarian cancer was validated by analyzing multiple independent cohorts in publicly available data sets. Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors. Functional assays in ovarian cancer cells revealed that BAIAP2L1 is involved in promoting cell proliferation and avoiding apoptosis. In conclusion, results of this study not only indicate that BAIAP2L1 can be used as a biomarker for human ovarian cancer but also reveal its role in cancer biology. Further elucidation of the role of BAIAP2L1 in context of the insulin receptor signaling pathways of cancer cells is warranted for developing cancer therapeutics by targeting cancer-specific metabolism.

No MeSH data available.


Related in: MedlinePlus

The mRNA profiles of BAIAP2L1 on tissues and cell lines.(A) The Cancer Profiling Array II (BD Clontech) of cDNA derived from normal tissues (N) and tumor tissues (T) of ovary, uterus, and cervix. Quantitation of the mRNA of tumor and normal tissues of different sites are shown at the right panel. BAIAP2L1 expression in tumors was significantly higher than that in normal tissues (** P<0.01). (B) BAIAP2L1 RNA expression in ovarian cancer cell lines was higher than normal cell lines. Ovarian cancer lines included serous type (SKOV3), endometrioid type (TOV112D, MDAH2774), and clear cell type (TOV21G). HFL1 is human normal lung fibroblast cell, and T/G HA-VSMC is human normal aorta smooth muscle cells. Abbreviations: T, tumor; N, normal.
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pone.0133081.g001: The mRNA profiles of BAIAP2L1 on tissues and cell lines.(A) The Cancer Profiling Array II (BD Clontech) of cDNA derived from normal tissues (N) and tumor tissues (T) of ovary, uterus, and cervix. Quantitation of the mRNA of tumor and normal tissues of different sites are shown at the right panel. BAIAP2L1 expression in tumors was significantly higher than that in normal tissues (** P<0.01). (B) BAIAP2L1 RNA expression in ovarian cancer cell lines was higher than normal cell lines. Ovarian cancer lines included serous type (SKOV3), endometrioid type (TOV112D, MDAH2774), and clear cell type (TOV21G). HFL1 is human normal lung fibroblast cell, and T/G HA-VSMC is human normal aorta smooth muscle cells. Abbreviations: T, tumor; N, normal.

Mentions: The 10 ovarian tumors specimens studied comprised of 6 malignant cancers, 2 borderline serous cystadenoma (coordinate #E and N), and 2 leiomyoma (coordinates #F and J) (Fig 1A). Other gynecologic cancer and normal tissues of uterine and cervical origins were also determined. In all three tissue types, BAIAP2L1 expression levels in tumor tissues were significantly higher than normal tissues. Similarly, mRNA expression of BAIAP2L1 in ovarian cancer cell lines was higher than normal cell lines (Fig 1B). In agreement with RNA expression results, BAIAP2L1 protein expression is higher in ovarian cancer than in normal tissues, as shown in FDA805-1and 805–2 tissue arrays (Fig 2).


BAI1-Associated Protein 2-Like 1 (BAIAP2L1) Is a Potential Biomarker in Ovarian Cancer.

Chao A, Tsai CL, Jung SM, Chuang WC, Kao C, Hsu A, Chen SH, Lin CY, Lee YC, Lee YS, Wang TH, Wang HS, Lai CH - PLoS ONE (2015)

The mRNA profiles of BAIAP2L1 on tissues and cell lines.(A) The Cancer Profiling Array II (BD Clontech) of cDNA derived from normal tissues (N) and tumor tissues (T) of ovary, uterus, and cervix. Quantitation of the mRNA of tumor and normal tissues of different sites are shown at the right panel. BAIAP2L1 expression in tumors was significantly higher than that in normal tissues (** P<0.01). (B) BAIAP2L1 RNA expression in ovarian cancer cell lines was higher than normal cell lines. Ovarian cancer lines included serous type (SKOV3), endometrioid type (TOV112D, MDAH2774), and clear cell type (TOV21G). HFL1 is human normal lung fibroblast cell, and T/G HA-VSMC is human normal aorta smooth muscle cells. Abbreviations: T, tumor; N, normal.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519316&req=5

pone.0133081.g001: The mRNA profiles of BAIAP2L1 on tissues and cell lines.(A) The Cancer Profiling Array II (BD Clontech) of cDNA derived from normal tissues (N) and tumor tissues (T) of ovary, uterus, and cervix. Quantitation of the mRNA of tumor and normal tissues of different sites are shown at the right panel. BAIAP2L1 expression in tumors was significantly higher than that in normal tissues (** P<0.01). (B) BAIAP2L1 RNA expression in ovarian cancer cell lines was higher than normal cell lines. Ovarian cancer lines included serous type (SKOV3), endometrioid type (TOV112D, MDAH2774), and clear cell type (TOV21G). HFL1 is human normal lung fibroblast cell, and T/G HA-VSMC is human normal aorta smooth muscle cells. Abbreviations: T, tumor; N, normal.
Mentions: The 10 ovarian tumors specimens studied comprised of 6 malignant cancers, 2 borderline serous cystadenoma (coordinate #E and N), and 2 leiomyoma (coordinates #F and J) (Fig 1A). Other gynecologic cancer and normal tissues of uterine and cervical origins were also determined. In all three tissue types, BAIAP2L1 expression levels in tumor tissues were significantly higher than normal tissues. Similarly, mRNA expression of BAIAP2L1 in ovarian cancer cell lines was higher than normal cell lines (Fig 1B). In agreement with RNA expression results, BAIAP2L1 protein expression is higher in ovarian cancer than in normal tissues, as shown in FDA805-1and 805–2 tissue arrays (Fig 2).

Bottom Line: Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration.BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma.Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.

ABSTRACT
Brain-specific angiogenesis inhibitor 1 (BAI1)-associated protein 2-like 1 (BAIAP2L1), also known as insulin receptor tyrosine kinase substrate (IRTKS), is involved in plasma membrane protrusion and actin formation during cell morphogenesis and migration. BAIAP2L1 is recently reported to promote cell proliferation through activation of the EGFR-ERK pathway in hepatocellular carcinoma. In this study, we report the first comprehensive study of BAIAP2L1 upregulation in human ovarian cancer. Upregulation of BAIAP2L1 in ovarian tumors was first found during RNA screening and confirmed by immunohistochemical studies on ovarian cancers and other cancer types. Significant upregulation of BAIAP2L1 in ovarian cancer was validated by analyzing multiple independent cohorts in publicly available data sets. Furthermore, BAIAP2L1 protein expression in metastatic lesions was higher than the corresponding primary tumors. Functional assays in ovarian cancer cells revealed that BAIAP2L1 is involved in promoting cell proliferation and avoiding apoptosis. In conclusion, results of this study not only indicate that BAIAP2L1 can be used as a biomarker for human ovarian cancer but also reveal its role in cancer biology. Further elucidation of the role of BAIAP2L1 in context of the insulin receptor signaling pathways of cancer cells is warranted for developing cancer therapeutics by targeting cancer-specific metabolism.

No MeSH data available.


Related in: MedlinePlus