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Cold Atmospheric Plasma Modified Electrospun Scaffolds with Embedded Microspheres for Improved Cartilage Regeneration.

Zhu W, Castro NJ, Cheng X, Keidar M, Zhang LG - PLoS ONE (2015)

Bottom Line: However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties.More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production).By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, District of Columbia, United States of America.

ABSTRACT
Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to inherent low cell density and the absence of a vasculature network. Tissue engineered cartilage scaffolds show promise for cartilage repair. However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties. Therefore, the objective of this study is to develop a novel biomimetic and bioactive electrospun cartilage substitute by integrating cold atmospheric plasma (CAP) treatment with sustained growth factor delivery microspheres. Specifically, CAP was applied to a poly(ε-caprolactone) electrospun scaffold with homogeneously distributed bioactive factors (transforming growth factor-β1 and bovine serum albumin) loaded poly(lactic-co-glycolic) acid microspheres. We have shown that CAP treatment renders electrospun scaffolds more hydrophilic thus facilitating vitronectin adsorption. More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production). Furthermore, CAP can substantially enhance 3D cell infiltration (over two-fold increase in infiltration depth after 1 day of culture) in the scaffolds. By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

No MeSH data available.


Related in: MedlinePlus

Type II collagen and total collagen synthesis.Scaffolds with CAP treated, microspheres embedded, and both CAP and microspheres modification promoted (A) type II collagen and (B) total collagen synthesis relative to bare control, data are mean ± standard error of the mean, N = 3, *p < 0.05, &p<0.1.
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pone.0134729.g011: Type II collagen and total collagen synthesis.Scaffolds with CAP treated, microspheres embedded, and both CAP and microspheres modification promoted (A) type II collagen and (B) total collagen synthesis relative to bare control, data are mean ± standard error of the mean, N = 3, *p < 0.05, &p<0.1.

Mentions: In addition to total GAG content, type II collagen (another important indicator of MSC chondrogenesis) was evaluated. As shown in Fig 11A, type II collagen synthesis was significantly improved after each time point for the scaffolds with CAP treatment, embedded microspheres, and both CAP and embedded microsphere modification when compared to control. Additionally, total collagen quantification (Fig 11B) demonstrated that scaffolds with CAP treatment and embedded microspheres outperformed the bare control after three weeks of culture. These results help to illustrate the synergistic promotion of CAP and bioactive microspheres for regulated MSC chondrogenic differentiation in vitro.


Cold Atmospheric Plasma Modified Electrospun Scaffolds with Embedded Microspheres for Improved Cartilage Regeneration.

Zhu W, Castro NJ, Cheng X, Keidar M, Zhang LG - PLoS ONE (2015)

Type II collagen and total collagen synthesis.Scaffolds with CAP treated, microspheres embedded, and both CAP and microspheres modification promoted (A) type II collagen and (B) total collagen synthesis relative to bare control, data are mean ± standard error of the mean, N = 3, *p < 0.05, &p<0.1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519315&req=5

pone.0134729.g011: Type II collagen and total collagen synthesis.Scaffolds with CAP treated, microspheres embedded, and both CAP and microspheres modification promoted (A) type II collagen and (B) total collagen synthesis relative to bare control, data are mean ± standard error of the mean, N = 3, *p < 0.05, &p<0.1.
Mentions: In addition to total GAG content, type II collagen (another important indicator of MSC chondrogenesis) was evaluated. As shown in Fig 11A, type II collagen synthesis was significantly improved after each time point for the scaffolds with CAP treatment, embedded microspheres, and both CAP and embedded microsphere modification when compared to control. Additionally, total collagen quantification (Fig 11B) demonstrated that scaffolds with CAP treatment and embedded microspheres outperformed the bare control after three weeks of culture. These results help to illustrate the synergistic promotion of CAP and bioactive microspheres for regulated MSC chondrogenic differentiation in vitro.

Bottom Line: However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties.More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production).By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, District of Columbia, United States of America.

ABSTRACT
Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to inherent low cell density and the absence of a vasculature network. Tissue engineered cartilage scaffolds show promise for cartilage repair. However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties. Therefore, the objective of this study is to develop a novel biomimetic and bioactive electrospun cartilage substitute by integrating cold atmospheric plasma (CAP) treatment with sustained growth factor delivery microspheres. Specifically, CAP was applied to a poly(ε-caprolactone) electrospun scaffold with homogeneously distributed bioactive factors (transforming growth factor-β1 and bovine serum albumin) loaded poly(lactic-co-glycolic) acid microspheres. We have shown that CAP treatment renders electrospun scaffolds more hydrophilic thus facilitating vitronectin adsorption. More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production). Furthermore, CAP can substantially enhance 3D cell infiltration (over two-fold increase in infiltration depth after 1 day of culture) in the scaffolds. By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

No MeSH data available.


Related in: MedlinePlus