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Cold Atmospheric Plasma Modified Electrospun Scaffolds with Embedded Microspheres for Improved Cartilage Regeneration.

Zhu W, Castro NJ, Cheng X, Keidar M, Zhang LG - PLoS ONE (2015)

Bottom Line: However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties.More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production).By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, District of Columbia, United States of America.

ABSTRACT
Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to inherent low cell density and the absence of a vasculature network. Tissue engineered cartilage scaffolds show promise for cartilage repair. However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties. Therefore, the objective of this study is to develop a novel biomimetic and bioactive electrospun cartilage substitute by integrating cold atmospheric plasma (CAP) treatment with sustained growth factor delivery microspheres. Specifically, CAP was applied to a poly(ε-caprolactone) electrospun scaffold with homogeneously distributed bioactive factors (transforming growth factor-β1 and bovine serum albumin) loaded poly(lactic-co-glycolic) acid microspheres. We have shown that CAP treatment renders electrospun scaffolds more hydrophilic thus facilitating vitronectin adsorption. More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production). Furthermore, CAP can substantially enhance 3D cell infiltration (over two-fold increase in infiltration depth after 1 day of culture) in the scaffolds. By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

No MeSH data available.


Related in: MedlinePlus

Compressive modulus of CAP modified and unmodified (0 min) scaffolds.Sample thickness is 0.5 mm, data are mean ± standard error of the mean, n = 3, *p<0.05.
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pone.0134729.g006: Compressive modulus of CAP modified and unmodified (0 min) scaffolds.Sample thickness is 0.5 mm, data are mean ± standard error of the mean, n = 3, *p<0.05.

Mentions: Fig 6 shows the compressive modulus of CAP modified and unmodified scaffolds with embedded microspheres. The unmodified scaffold exhibited a compressive modulus of 248 KPa with CAP modification slightly decreasing the compressive modulus. Specifically, after 1 min of CAP exposure the scaffold exhibited a compressive modulus of 201 KPa. With increasing CAP treatment, the compressive modulus decreased to 137 and 136 KPa corresponding to 3 min and 5 min exposure time, respectively.


Cold Atmospheric Plasma Modified Electrospun Scaffolds with Embedded Microspheres for Improved Cartilage Regeneration.

Zhu W, Castro NJ, Cheng X, Keidar M, Zhang LG - PLoS ONE (2015)

Compressive modulus of CAP modified and unmodified (0 min) scaffolds.Sample thickness is 0.5 mm, data are mean ± standard error of the mean, n = 3, *p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519315&req=5

pone.0134729.g006: Compressive modulus of CAP modified and unmodified (0 min) scaffolds.Sample thickness is 0.5 mm, data are mean ± standard error of the mean, n = 3, *p<0.05.
Mentions: Fig 6 shows the compressive modulus of CAP modified and unmodified scaffolds with embedded microspheres. The unmodified scaffold exhibited a compressive modulus of 248 KPa with CAP modification slightly decreasing the compressive modulus. Specifically, after 1 min of CAP exposure the scaffold exhibited a compressive modulus of 201 KPa. With increasing CAP treatment, the compressive modulus decreased to 137 and 136 KPa corresponding to 3 min and 5 min exposure time, respectively.

Bottom Line: However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties.More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production).By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, District of Columbia, United States of America.

ABSTRACT
Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to inherent low cell density and the absence of a vasculature network. Tissue engineered cartilage scaffolds show promise for cartilage repair. However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties. Therefore, the objective of this study is to develop a novel biomimetic and bioactive electrospun cartilage substitute by integrating cold atmospheric plasma (CAP) treatment with sustained growth factor delivery microspheres. Specifically, CAP was applied to a poly(ε-caprolactone) electrospun scaffold with homogeneously distributed bioactive factors (transforming growth factor-β1 and bovine serum albumin) loaded poly(lactic-co-glycolic) acid microspheres. We have shown that CAP treatment renders electrospun scaffolds more hydrophilic thus facilitating vitronectin adsorption. More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production). Furthermore, CAP can substantially enhance 3D cell infiltration (over two-fold increase in infiltration depth after 1 day of culture) in the scaffolds. By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

No MeSH data available.


Related in: MedlinePlus