Limits...
Cold Atmospheric Plasma Modified Electrospun Scaffolds with Embedded Microspheres for Improved Cartilage Regeneration.

Zhu W, Castro NJ, Cheng X, Keidar M, Zhang LG - PLoS ONE (2015)

Bottom Line: However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties.More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production).By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, District of Columbia, United States of America.

ABSTRACT
Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to inherent low cell density and the absence of a vasculature network. Tissue engineered cartilage scaffolds show promise for cartilage repair. However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties. Therefore, the objective of this study is to develop a novel biomimetic and bioactive electrospun cartilage substitute by integrating cold atmospheric plasma (CAP) treatment with sustained growth factor delivery microspheres. Specifically, CAP was applied to a poly(ε-caprolactone) electrospun scaffold with homogeneously distributed bioactive factors (transforming growth factor-β1 and bovine serum albumin) loaded poly(lactic-co-glycolic) acid microspheres. We have shown that CAP treatment renders electrospun scaffolds more hydrophilic thus facilitating vitronectin adsorption. More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production). Furthermore, CAP can substantially enhance 3D cell infiltration (over two-fold increase in infiltration depth after 1 day of culture) in the scaffolds. By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

No MeSH data available.


Related in: MedlinePlus

Contact angle comparison of scaffolds with and without CAP treatment.Data are mean ± standard error of the mean, n = 3; *p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4519315&req=5

pone.0134729.g004: Contact angle comparison of scaffolds with and without CAP treatment.Data are mean ± standard error of the mean, n = 3; *p<0.05.

Mentions: In addition to morphology, the surface properties of scaffolds including hydrophilicity and protein adsorption significantly influence scaffold performance and cytocompatibility [25]. Therefore, the hydrophilicity (contact angle) of scaffolds exposed under various CAP treatment times was measured (Fig 4). Scaffolds without CAP treatment exhibited a contact angle value of 117.3° indicating a hydrophobic surface. After CAP treatment, we found the contact angle significantly decreased and exhibited a linear dependence to exposure time. After 1 min CAP treatment, a slight decrease (92.7°) of contact angle was observed. After 3 min CAP exposure, the contact angle sharply decreased by 61.3% (34.8° contact angle) when compared to an untreated control, suggesting the induction of a hydrophilic surface. Extended exposure (up to 5 min) further increased surface hydrophilicity where the contact angle decreased to 14.2°.


Cold Atmospheric Plasma Modified Electrospun Scaffolds with Embedded Microspheres for Improved Cartilage Regeneration.

Zhu W, Castro NJ, Cheng X, Keidar M, Zhang LG - PLoS ONE (2015)

Contact angle comparison of scaffolds with and without CAP treatment.Data are mean ± standard error of the mean, n = 3; *p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519315&req=5

pone.0134729.g004: Contact angle comparison of scaffolds with and without CAP treatment.Data are mean ± standard error of the mean, n = 3; *p<0.05.
Mentions: In addition to morphology, the surface properties of scaffolds including hydrophilicity and protein adsorption significantly influence scaffold performance and cytocompatibility [25]. Therefore, the hydrophilicity (contact angle) of scaffolds exposed under various CAP treatment times was measured (Fig 4). Scaffolds without CAP treatment exhibited a contact angle value of 117.3° indicating a hydrophobic surface. After CAP treatment, we found the contact angle significantly decreased and exhibited a linear dependence to exposure time. After 1 min CAP treatment, a slight decrease (92.7°) of contact angle was observed. After 3 min CAP exposure, the contact angle sharply decreased by 61.3% (34.8° contact angle) when compared to an untreated control, suggesting the induction of a hydrophilic surface. Extended exposure (up to 5 min) further increased surface hydrophilicity where the contact angle decreased to 14.2°.

Bottom Line: However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties.More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production).By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, District of Columbia, United States of America.

ABSTRACT
Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to inherent low cell density and the absence of a vasculature network. Tissue engineered cartilage scaffolds show promise for cartilage repair. However, there still remains a lack of ideal biomimetic tissue scaffolds which effectively stimulate cartilage regeneration with appropriate functional properties. Therefore, the objective of this study is to develop a novel biomimetic and bioactive electrospun cartilage substitute by integrating cold atmospheric plasma (CAP) treatment with sustained growth factor delivery microspheres. Specifically, CAP was applied to a poly(ε-caprolactone) electrospun scaffold with homogeneously distributed bioactive factors (transforming growth factor-β1 and bovine serum albumin) loaded poly(lactic-co-glycolic) acid microspheres. We have shown that CAP treatment renders electrospun scaffolds more hydrophilic thus facilitating vitronectin adsorption. More importantly, our results demonstrate, for the first time, CAP and microspheres can synergistically enhance stem cell growth as well as improve chondrogenic differentiation of human marrow-derived mesenchymal stem cells (such as increased glycosaminoglycan, type II collagen, and total collagen production). Furthermore, CAP can substantially enhance 3D cell infiltration (over two-fold increase in infiltration depth after 1 day of culture) in the scaffolds. By integrating CAP, sustained bioactive factor loaded microspheres, and electrospinning, we have fabricated a promising bioactive scaffold for cartilage regeneration.

No MeSH data available.


Related in: MedlinePlus