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Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus

Schematic model and systematic pathway to Pi starvation, its tolerance and homeostatic mechanisms in tomato plants.Pi starvation affects the cellular processes like energy production, photosynthesis, photorespiration, and various metabolic pathways (transcription/translation) whereas, the regulatory pathways to defend Pi starvation for tolerance and its homeostasis were regulated in tomato plants as described in our proteome data and transcript levels.
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pone.0134103.g006: Schematic model and systematic pathway to Pi starvation, its tolerance and homeostatic mechanisms in tomato plants.Pi starvation affects the cellular processes like energy production, photosynthesis, photorespiration, and various metabolic pathways (transcription/translation) whereas, the regulatory pathways to defend Pi starvation for tolerance and its homeostasis were regulated in tomato plants as described in our proteome data and transcript levels.

Mentions: Tomato is one of the highest consumed fruit throughout the world, one of the major challenge for its high quality and production is adaptive mechanisms of internal mineral deficiency. Among mineral deficiencies, Pi is naturally limiting in a soil due to a poor mobility. The major challenge in the present study was to explain Pi acquisition and its homeostasis at a proteome level. A number of proteins with various functions were identified. Some of the identified proteins were already known to be associated with abiotic stress but have not been detected in response to Pi starvation. The most significant proteins were identified as photosynthesis, defense responses, protein transcription/ translation related proteins. The identified proteins were mostly up-regulated in 2-DE gels which indicate tolerance or homeostasis of Pi under Pi starvation. Moreover, transcriptional regulation of Pi-transporter gene expressions and their localization regulated directly or indirectly to Pi acquisition which appeared to a very important part in homeostasis of Pi-starvation. A possible regulatory network for adaptation and homeostasis of Pi-starvation in tomato plants has been given diagrammatically in Fig 6 based on the results of our physiological, proteomic and transcriptional regulations.


Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Schematic model and systematic pathway to Pi starvation, its tolerance and homeostatic mechanisms in tomato plants.Pi starvation affects the cellular processes like energy production, photosynthesis, photorespiration, and various metabolic pathways (transcription/translation) whereas, the regulatory pathways to defend Pi starvation for tolerance and its homeostasis were regulated in tomato plants as described in our proteome data and transcript levels.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519287&req=5

pone.0134103.g006: Schematic model and systematic pathway to Pi starvation, its tolerance and homeostatic mechanisms in tomato plants.Pi starvation affects the cellular processes like energy production, photosynthesis, photorespiration, and various metabolic pathways (transcription/translation) whereas, the regulatory pathways to defend Pi starvation for tolerance and its homeostasis were regulated in tomato plants as described in our proteome data and transcript levels.
Mentions: Tomato is one of the highest consumed fruit throughout the world, one of the major challenge for its high quality and production is adaptive mechanisms of internal mineral deficiency. Among mineral deficiencies, Pi is naturally limiting in a soil due to a poor mobility. The major challenge in the present study was to explain Pi acquisition and its homeostasis at a proteome level. A number of proteins with various functions were identified. Some of the identified proteins were already known to be associated with abiotic stress but have not been detected in response to Pi starvation. The most significant proteins were identified as photosynthesis, defense responses, protein transcription/ translation related proteins. The identified proteins were mostly up-regulated in 2-DE gels which indicate tolerance or homeostasis of Pi under Pi starvation. Moreover, transcriptional regulation of Pi-transporter gene expressions and their localization regulated directly or indirectly to Pi acquisition which appeared to a very important part in homeostasis of Pi-starvation. A possible regulatory network for adaptation and homeostasis of Pi-starvation in tomato plants has been given diagrammatically in Fig 6 based on the results of our physiological, proteomic and transcriptional regulations.

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus