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Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus

Relative expression and localization of phosphate transporters in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Relative expression of lePT1 and lePT2; Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test (B) Localization of lePT1 and lePT2 observed under light microscope. E symbolizes epidermis, PP symbolizes palisade parenchyma, SP symbolizes sponge parenchyma, and T symbolizes trichome.
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pone.0134103.g005: Relative expression and localization of phosphate transporters in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Relative expression of lePT1 and lePT2; Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test (B) Localization of lePT1 and lePT2 observed under light microscope. E symbolizes epidermis, PP symbolizes palisade parenchyma, SP symbolizes sponge parenchyma, and T symbolizes trichome.

Mentions: The relative expression of Pi transporter gene (lePT1) in tomato leaves was expressed significantly in higher amounts to 2.5 folds under Pi starvation (Fig 5A) compared to Pi-sufficient leaves. Similarly, the relative expression of lePT2 gene significantly increased to 6 folds under Pi starvation (Fig 5B) compared to Pi-sufficient leaves. The increased expressions of lePT1 and lePT2 suggested a correlation between the amounts of phosphorus present in the medium in which tomato plants were grown.


Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Relative expression and localization of phosphate transporters in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Relative expression of lePT1 and lePT2; Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test (B) Localization of lePT1 and lePT2 observed under light microscope. E symbolizes epidermis, PP symbolizes palisade parenchyma, SP symbolizes sponge parenchyma, and T symbolizes trichome.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519287&req=5

pone.0134103.g005: Relative expression and localization of phosphate transporters in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Relative expression of lePT1 and lePT2; Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test (B) Localization of lePT1 and lePT2 observed under light microscope. E symbolizes epidermis, PP symbolizes palisade parenchyma, SP symbolizes sponge parenchyma, and T symbolizes trichome.
Mentions: The relative expression of Pi transporter gene (lePT1) in tomato leaves was expressed significantly in higher amounts to 2.5 folds under Pi starvation (Fig 5A) compared to Pi-sufficient leaves. Similarly, the relative expression of lePT2 gene significantly increased to 6 folds under Pi starvation (Fig 5B) compared to Pi-sufficient leaves. The increased expressions of lePT1 and lePT2 suggested a correlation between the amounts of phosphorus present in the medium in which tomato plants were grown.

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus