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Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus

Protein profile in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Comparative analysis of protein profile by first dimension SDS-PAGE for analysis of enrichment of proteins (B) Comparison of 2-DE gel maps of proteins. The proteins were extracted using a commercial available kit and 100 μg protein samples were separated by isoelectric focusing (IEF) using 11 cm pH 4–7 IPG strips. The focused strips were placed on a 12% polyacrylamide gel for second-dimensional separation and stained with silver stain. The gel image analysis was carried out using PDQuest software. The encircled protein spots marked with numbers were differentially expressed. All differentially protein spots were identified by MALDI-TOF MS listed in Table 1.
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pone.0134103.g003: Protein profile in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Comparative analysis of protein profile by first dimension SDS-PAGE for analysis of enrichment of proteins (B) Comparison of 2-DE gel maps of proteins. The proteins were extracted using a commercial available kit and 100 μg protein samples were separated by isoelectric focusing (IEF) using 11 cm pH 4–7 IPG strips. The focused strips were placed on a 12% polyacrylamide gel for second-dimensional separation and stained with silver stain. The gel image analysis was carried out using PDQuest software. The encircled protein spots marked with numbers were differentially expressed. All differentially protein spots were identified by MALDI-TOF MS listed in Table 1.

Mentions: The relative total protein profile were first analyzed by first dimension sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) to check the enrichment of proteins (Fig 3A). The detailed comparative 2-DE images were analyzed by PD-Quest software (Bio-Rad, Hercules, CA, USA) in the leaves of tomato subjected to Pi starvation (Fig 3B). About 600 protein spots were detected on each 2-DE gels (Fig 4A). Among 600 protein spots, 46 protein spots were differentially expressed (Figs 3B and 4A) between +Pi and–Pi treatments. It was observed that most of the protein spots in–Pi were up-regulated compared to +Pi treatments. A possible number of proteins in +Pi was also observed to be absent compared to–Pi 2-DE gels. The results indicate that Pi-starved treatments resulted in major proteomic changes in the tomato leaves.


Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Protein profile in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Comparative analysis of protein profile by first dimension SDS-PAGE for analysis of enrichment of proteins (B) Comparison of 2-DE gel maps of proteins. The proteins were extracted using a commercial available kit and 100 μg protein samples were separated by isoelectric focusing (IEF) using 11 cm pH 4–7 IPG strips. The focused strips were placed on a 12% polyacrylamide gel for second-dimensional separation and stained with silver stain. The gel image analysis was carried out using PDQuest software. The encircled protein spots marked with numbers were differentially expressed. All differentially protein spots were identified by MALDI-TOF MS listed in Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519287&req=5

pone.0134103.g003: Protein profile in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Comparative analysis of protein profile by first dimension SDS-PAGE for analysis of enrichment of proteins (B) Comparison of 2-DE gel maps of proteins. The proteins were extracted using a commercial available kit and 100 μg protein samples were separated by isoelectric focusing (IEF) using 11 cm pH 4–7 IPG strips. The focused strips were placed on a 12% polyacrylamide gel for second-dimensional separation and stained with silver stain. The gel image analysis was carried out using PDQuest software. The encircled protein spots marked with numbers were differentially expressed. All differentially protein spots were identified by MALDI-TOF MS listed in Table 1.
Mentions: The relative total protein profile were first analyzed by first dimension sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) to check the enrichment of proteins (Fig 3A). The detailed comparative 2-DE images were analyzed by PD-Quest software (Bio-Rad, Hercules, CA, USA) in the leaves of tomato subjected to Pi starvation (Fig 3B). About 600 protein spots were detected on each 2-DE gels (Fig 4A). Among 600 protein spots, 46 protein spots were differentially expressed (Figs 3B and 4A) between +Pi and–Pi treatments. It was observed that most of the protein spots in–Pi were up-regulated compared to +Pi treatments. A possible number of proteins in +Pi was also observed to be absent compared to–Pi 2-DE gels. The results indicate that Pi-starved treatments resulted in major proteomic changes in the tomato leaves.

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus