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Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus

Histochemical localization in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) H2O2 by DAB staining (B) O2-1 by NBT staining, dark brownish parts indicate localization of H2O2 and blue parts indicate localization of O2-1.
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pone.0134103.g002: Histochemical localization in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) H2O2 by DAB staining (B) O2-1 by NBT staining, dark brownish parts indicate localization of H2O2 and blue parts indicate localization of O2-1.

Mentions: For the production of ROS, two important oxidative stress markers were visualized in situ in the form of H2O2 and O2-1 by histochemical methods. For visualizing H2O2 accumulation, the DAB reaction based on the formation of brownish parts was used. Pi-starved leaves displayed brownish staining compared to +Pi treatments (Fig 2A). The O2-1 was studied by a reaction with nitro-blue tetrazolium (NBT), giving rise to dark-blue spots of blue formazan. In Pi starved leaves, dark blue spotted areas were prevalent (Fig 2B) whereas no blue spotted area was observed in Pi-sufficient leaves.


Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Histochemical localization in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) H2O2 by DAB staining (B) O2-1 by NBT staining, dark brownish parts indicate localization of H2O2 and blue parts indicate localization of O2-1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519287&req=5

pone.0134103.g002: Histochemical localization in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) H2O2 by DAB staining (B) O2-1 by NBT staining, dark brownish parts indicate localization of H2O2 and blue parts indicate localization of O2-1.
Mentions: For the production of ROS, two important oxidative stress markers were visualized in situ in the form of H2O2 and O2-1 by histochemical methods. For visualizing H2O2 accumulation, the DAB reaction based on the formation of brownish parts was used. Pi-starved leaves displayed brownish staining compared to +Pi treatments (Fig 2A). The O2-1 was studied by a reaction with nitro-blue tetrazolium (NBT), giving rise to dark-blue spots of blue formazan. In Pi starved leaves, dark blue spotted areas were prevalent (Fig 2B) whereas no blue spotted area was observed in Pi-sufficient leaves.

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus