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Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus

Phenotypic and physiological indices in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Phenotypic differences (B) Pi concentration (C) total chlorophyll and (D) carotenoid content. Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test.
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pone.0134103.g001: Phenotypic and physiological indices in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Phenotypic differences (B) Pi concentration (C) total chlorophyll and (D) carotenoid content. Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test.

Mentions: After 10 days of Pi starvation, the tomato leaves displayed apparent Pi deficiency symptoms, including yellowing of leaves (chlorotic lesions) (Fig 1A) and a significant decline in P content (Fig 1B). Pi starvation significantly decreased photosynthetic pigments (total chlorophyll and carotenoid content) to 70% compared to Pi sufficient plants (Fig 1C and 1D).


Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.).

Muneer S, Jeong BR - PLoS ONE (2015)

Phenotypic and physiological indices in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Phenotypic differences (B) Pi concentration (C) total chlorophyll and (D) carotenoid content. Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519287&req=5

pone.0134103.g001: Phenotypic and physiological indices in leaves of tomato (Solanum lycopersicum L.) to Pi starvation.One week after germination tomato plants were supplied with sufficient Pi (1 M KH2PO4) or deficient Pi (0 M KH2PO4) for 10 days. (A) Phenotypic differences (B) Pi concentration (C) total chlorophyll and (D) carotenoid content. Vertical bars indicate Mean±SE of the means for n = 3. Means denoted by the different letter are significantly different at P≤0.05 according to the Tukey’s studentized range test.
Mentions: After 10 days of Pi starvation, the tomato leaves displayed apparent Pi deficiency symptoms, including yellowing of leaves (chlorotic lesions) (Fig 1A) and a significant decline in P content (Fig 1B). Pi starvation significantly decreased photosynthetic pigments (total chlorophyll and carotenoid content) to 70% compared to Pi sufficient plants (Fig 1C and 1D).

Bottom Line: The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments.Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments.Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University, Jinju, 660-701, South Korea.

ABSTRACT
Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under -Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under -Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to -Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

No MeSH data available.


Related in: MedlinePlus