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Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.

Musser JM, Heatley JJ, Koinis AV, Suchodolski PF, Guo J, Escandon P, Tizard IR - PLoS ONE (2015)

Bottom Line: We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells.The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition.This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

View Article: PubMed Central - PubMed

Affiliation: Schubot Exotic Bird Health Center, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America; Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America.

ABSTRACT
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

No MeSH data available.


Related in: MedlinePlus

Potential anti-viral synergism of baicalein and ribavirin on PaBV-4.Duck embryonic fibroblasts infected with 7 (white hashed bars) or 64 (grey bars) ffu PaBV-4, incubated with 0.05 μg/mL baicalein, 5.0 μg/mL ribavirin, both drugs or neither. Cells were analyzed by qRT-PCR with results (mean ±SD) shown as the fold change in P gene mRNA expression relative to the expression of the groups treated with neither drug.
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pone.0134080.g007: Potential anti-viral synergism of baicalein and ribavirin on PaBV-4.Duck embryonic fibroblasts infected with 7 (white hashed bars) or 64 (grey bars) ffu PaBV-4, incubated with 0.05 μg/mL baicalein, 5.0 μg/mL ribavirin, both drugs or neither. Cells were analyzed by qRT-PCR with results (mean ±SD) shown as the fold change in P gene mRNA expression relative to the expression of the groups treated with neither drug.

Mentions: The effect of baicalein on the antiviral activity of ribavirin was investigated by comparing the expression of P gene mRNA with and without treatments. With the addition of 5.0 μg/ml ribavirin or with the combination treatment of ribavirin and baicalein, there was a 3.5 to 4.5 fold reduction in mRNA expression as compared with no treatment control, however combination treatment did not show mRNA decreasing more than ribavirin alone (Fig 7).


Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.

Musser JM, Heatley JJ, Koinis AV, Suchodolski PF, Guo J, Escandon P, Tizard IR - PLoS ONE (2015)

Potential anti-viral synergism of baicalein and ribavirin on PaBV-4.Duck embryonic fibroblasts infected with 7 (white hashed bars) or 64 (grey bars) ffu PaBV-4, incubated with 0.05 μg/mL baicalein, 5.0 μg/mL ribavirin, both drugs or neither. Cells were analyzed by qRT-PCR with results (mean ±SD) shown as the fold change in P gene mRNA expression relative to the expression of the groups treated with neither drug.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519282&req=5

pone.0134080.g007: Potential anti-viral synergism of baicalein and ribavirin on PaBV-4.Duck embryonic fibroblasts infected with 7 (white hashed bars) or 64 (grey bars) ffu PaBV-4, incubated with 0.05 μg/mL baicalein, 5.0 μg/mL ribavirin, both drugs or neither. Cells were analyzed by qRT-PCR with results (mean ±SD) shown as the fold change in P gene mRNA expression relative to the expression of the groups treated with neither drug.
Mentions: The effect of baicalein on the antiviral activity of ribavirin was investigated by comparing the expression of P gene mRNA with and without treatments. With the addition of 5.0 μg/ml ribavirin or with the combination treatment of ribavirin and baicalein, there was a 3.5 to 4.5 fold reduction in mRNA expression as compared with no treatment control, however combination treatment did not show mRNA decreasing more than ribavirin alone (Fig 7).

Bottom Line: We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells.The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition.This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

View Article: PubMed Central - PubMed

Affiliation: Schubot Exotic Bird Health Center, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America; Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America.

ABSTRACT
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

No MeSH data available.


Related in: MedlinePlus