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Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.

Musser JM, Heatley JJ, Koinis AV, Suchodolski PF, Guo J, Escandon P, Tizard IR - PLoS ONE (2015)

Bottom Line: We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells.The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition.This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

View Article: PubMed Central - PubMed

Affiliation: Schubot Exotic Bird Health Center, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America; Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America.

ABSTRACT
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

No MeSH data available.


Related in: MedlinePlus

Time dependency to reduce viral load.(A) PaBV-4 positive cell foci, assessed by indirect immunocytochemistry assay. (B) Percent area of infected cells, assessed by indirect immunocytochemistry assay. DEF infected with 64 ffu (black circle), 7 ffu of PaBV-4 (grey square), or no virus (white triangle), incubated for 0, 1, 2, or 5 days with 25μg/mL ribavirin. Data presents as median ± 25th and 75th percentile.
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pone.0134080.g005: Time dependency to reduce viral load.(A) PaBV-4 positive cell foci, assessed by indirect immunocytochemistry assay. (B) Percent area of infected cells, assessed by indirect immunocytochemistry assay. DEF infected with 64 ffu (black circle), 7 ffu of PaBV-4 (grey square), or no virus (white triangle), incubated for 0, 1, 2, or 5 days with 25μg/mL ribavirin. Data presents as median ± 25th and 75th percentile.

Mentions: We investigated treatment duration time needed to decrease the viral load of PaBV-4 infected cells. After only 24 hours of exposure to 25 μg/mL ribavirin, followed by 4 days of incubation without ribavirin, PaBV-4 positive foci numbers and percent area of infected cells were less than in the untreated controls with virus (Fig 5).


Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.

Musser JM, Heatley JJ, Koinis AV, Suchodolski PF, Guo J, Escandon P, Tizard IR - PLoS ONE (2015)

Time dependency to reduce viral load.(A) PaBV-4 positive cell foci, assessed by indirect immunocytochemistry assay. (B) Percent area of infected cells, assessed by indirect immunocytochemistry assay. DEF infected with 64 ffu (black circle), 7 ffu of PaBV-4 (grey square), or no virus (white triangle), incubated for 0, 1, 2, or 5 days with 25μg/mL ribavirin. Data presents as median ± 25th and 75th percentile.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519282&req=5

pone.0134080.g005: Time dependency to reduce viral load.(A) PaBV-4 positive cell foci, assessed by indirect immunocytochemistry assay. (B) Percent area of infected cells, assessed by indirect immunocytochemistry assay. DEF infected with 64 ffu (black circle), 7 ffu of PaBV-4 (grey square), or no virus (white triangle), incubated for 0, 1, 2, or 5 days with 25μg/mL ribavirin. Data presents as median ± 25th and 75th percentile.
Mentions: We investigated treatment duration time needed to decrease the viral load of PaBV-4 infected cells. After only 24 hours of exposure to 25 μg/mL ribavirin, followed by 4 days of incubation without ribavirin, PaBV-4 positive foci numbers and percent area of infected cells were less than in the untreated controls with virus (Fig 5).

Bottom Line: We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells.The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition.This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

View Article: PubMed Central - PubMed

Affiliation: Schubot Exotic Bird Health Center, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America; Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America.

ABSTRACT
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

No MeSH data available.


Related in: MedlinePlus