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Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.

Musser JM, Heatley JJ, Koinis AV, Suchodolski PF, Guo J, Escandon P, Tizard IR - PLoS ONE (2015)

Bottom Line: We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells.The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition.This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

View Article: PubMed Central - PubMed

Affiliation: Schubot Exotic Bird Health Center, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America; Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America.

ABSTRACT
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity on duck embryonic fibroblasts.(A) Cytotoxicity following incubation with HPLC grade ribavirin (> 98% pure). (B) Cytotoxicity following incubation with ribavirin capsule contents. (C) Cytotoxicity following incubation with baicalein or guanosine. Data is depicted as median ± 25th and 75th percentiles.
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pone.0134080.g002: Cytotoxicity on duck embryonic fibroblasts.(A) Cytotoxicity following incubation with HPLC grade ribavirin (> 98% pure). (B) Cytotoxicity following incubation with ribavirin capsule contents. (C) Cytotoxicity following incubation with baicalein or guanosine. Data is depicted as median ± 25th and 75th percentiles.

Mentions: In order to examine for potential confounding factors of the antiviral agents and other compounds on DEF cell viability, an MTT assay was used to examine the cytotoxicity of the compounds on DEF cells. Cytotoxicity was not determined to be a confounding factor at the concentrations of ribavirin, baicalein, or guanosine used for the virus inhibition experiments in this study. When incubated with ≥98% pure ribavirin (Fig 2A) or contents of ribavirin 200 mg capsule (Fig 2B), cell viability for DEF cells was similar to control values across ribavirin concentrations used in the virus inhibition experiments. When DEF cells were incubated with baicalein or guanosine (Fig 2C), cell viability remained constant across the range of concentrations used in the virus inhibition experiments.


Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.

Musser JM, Heatley JJ, Koinis AV, Suchodolski PF, Guo J, Escandon P, Tizard IR - PLoS ONE (2015)

Cytotoxicity on duck embryonic fibroblasts.(A) Cytotoxicity following incubation with HPLC grade ribavirin (> 98% pure). (B) Cytotoxicity following incubation with ribavirin capsule contents. (C) Cytotoxicity following incubation with baicalein or guanosine. Data is depicted as median ± 25th and 75th percentiles.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4519282&req=5

pone.0134080.g002: Cytotoxicity on duck embryonic fibroblasts.(A) Cytotoxicity following incubation with HPLC grade ribavirin (> 98% pure). (B) Cytotoxicity following incubation with ribavirin capsule contents. (C) Cytotoxicity following incubation with baicalein or guanosine. Data is depicted as median ± 25th and 75th percentiles.
Mentions: In order to examine for potential confounding factors of the antiviral agents and other compounds on DEF cell viability, an MTT assay was used to examine the cytotoxicity of the compounds on DEF cells. Cytotoxicity was not determined to be a confounding factor at the concentrations of ribavirin, baicalein, or guanosine used for the virus inhibition experiments in this study. When incubated with ≥98% pure ribavirin (Fig 2A) or contents of ribavirin 200 mg capsule (Fig 2B), cell viability for DEF cells was similar to control values across ribavirin concentrations used in the virus inhibition experiments. When DEF cells were incubated with baicalein or guanosine (Fig 2C), cell viability remained constant across the range of concentrations used in the virus inhibition experiments.

Bottom Line: We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells.The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition.This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

View Article: PubMed Central - PubMed

Affiliation: Schubot Exotic Bird Health Center, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America; Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas, United States of America.

ABSTRACT
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.

No MeSH data available.


Related in: MedlinePlus