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Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs.

Mentzel CM, Anthon C, Jacobsen MJ, Karlskov-Mortensen P, Bruun CS, Jørgensen CB, Gorodkin J, Cirera S, Fredholm M - PLoS ONE (2015)

Bottom Line: Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans.They are involved in numerous biological processes and diseases, including obesity.The results of these studies in general confirm the trends found by RNAseq.

View Article: PubMed Central - PubMed

Affiliation: Animal Genetics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark; Center for non-coding RNA in Technology and Health, Computational Biology and Bioinformatics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark.

ABSTRACT
Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding to their 3'UTR. They are involved in numerous biological processes and diseases, including obesity. In this study we use a mixed breed pig model designed for obesity studies to investigate differentially expressed miRNAs in subcutaneous adipose tissue by RNA sequencing (RNAseq). Both male and female pigs are included to explore gender differences. The RNAseq study shows that the most highly expressed miRNAs are in accordance with comparable studies in pigs and humans. A total of six miRNAs are differentially expressed in subcutaneous adipose tissue between the lean and obese group of pigs, and in addition gender specific significant differential expression is observed for a number of miRNAs. The differentially expressed miRNAs have been verified using qPCR. The results of these studies in general confirm the trends found by RNAseq. Mir-9 and mir-124a are significantly differentially expressed with large fold changes in subcutaneous adipose tissue between lean and obese pigs. Mir-9 is more highly expressed in the obese pigs with a fold change of 10 and a p-value < 0.001. Mir-124a is more highly expressed in the obese pigs with a fold change of 114 and a p-value < 0.001. In addition, mir-124a is significantly higher expressed in abdominal adipose tissue in male pigs with a fold change of 119 and a p-value < 0.05. Both miRNAs are also significantly higher expressed in the liver of obese male pigs where mir-124a has a fold change of 12 and mir-9 has a fold change of 1.6, both with p-values < 0.05.

No MeSH data available.


Related in: MedlinePlus

Expression of mir-9 and mir-124a in Abdominal Adipose Tissue and Liver.qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the two microRNAs in all three tissues. P-values calculated by Student’s t-test.
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pone.0131650.g003: Expression of mir-9 and mir-124a in Abdominal Adipose Tissue and Liver.qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the two microRNAs in all three tissues. P-values calculated by Student’s t-test.

Mentions: To further study the expression of mir-9 and mir-124a, the two miRNAs with the largest fold changes between the lean and obese group in the subcutaneous adipose tissue, qPCR was also performed on cDNA from abdominal adipose tissue, liver and muscle from the same animals as in the study of subcutaneous adipose tissue. The data is presented as column scatter plots in Fig 3. Mir-9 and mir-124a were expressed in all three tissues.


Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs.

Mentzel CM, Anthon C, Jacobsen MJ, Karlskov-Mortensen P, Bruun CS, Jørgensen CB, Gorodkin J, Cirera S, Fredholm M - PLoS ONE (2015)

Expression of mir-9 and mir-124a in Abdominal Adipose Tissue and Liver.qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the two microRNAs in all three tissues. P-values calculated by Student’s t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519260&req=5

pone.0131650.g003: Expression of mir-9 and mir-124a in Abdominal Adipose Tissue and Liver.qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the two microRNAs in all three tissues. P-values calculated by Student’s t-test.
Mentions: To further study the expression of mir-9 and mir-124a, the two miRNAs with the largest fold changes between the lean and obese group in the subcutaneous adipose tissue, qPCR was also performed on cDNA from abdominal adipose tissue, liver and muscle from the same animals as in the study of subcutaneous adipose tissue. The data is presented as column scatter plots in Fig 3. Mir-9 and mir-124a were expressed in all three tissues.

Bottom Line: Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans.They are involved in numerous biological processes and diseases, including obesity.The results of these studies in general confirm the trends found by RNAseq.

View Article: PubMed Central - PubMed

Affiliation: Animal Genetics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark; Center for non-coding RNA in Technology and Health, Computational Biology and Bioinformatics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark.

ABSTRACT
Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding to their 3'UTR. They are involved in numerous biological processes and diseases, including obesity. In this study we use a mixed breed pig model designed for obesity studies to investigate differentially expressed miRNAs in subcutaneous adipose tissue by RNA sequencing (RNAseq). Both male and female pigs are included to explore gender differences. The RNAseq study shows that the most highly expressed miRNAs are in accordance with comparable studies in pigs and humans. A total of six miRNAs are differentially expressed in subcutaneous adipose tissue between the lean and obese group of pigs, and in addition gender specific significant differential expression is observed for a number of miRNAs. The differentially expressed miRNAs have been verified using qPCR. The results of these studies in general confirm the trends found by RNAseq. Mir-9 and mir-124a are significantly differentially expressed with large fold changes in subcutaneous adipose tissue between lean and obese pigs. Mir-9 is more highly expressed in the obese pigs with a fold change of 10 and a p-value < 0.001. Mir-124a is more highly expressed in the obese pigs with a fold change of 114 and a p-value < 0.001. In addition, mir-124a is significantly higher expressed in abdominal adipose tissue in male pigs with a fold change of 119 and a p-value < 0.05. Both miRNAs are also significantly higher expressed in the liver of obese male pigs where mir-124a has a fold change of 12 and mir-9 has a fold change of 1.6, both with p-values < 0.05.

No MeSH data available.


Related in: MedlinePlus