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Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs.

Mentzel CM, Anthon C, Jacobsen MJ, Karlskov-Mortensen P, Bruun CS, Jørgensen CB, Gorodkin J, Cirera S, Fredholm M - PLoS ONE (2015)

Bottom Line: Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans.They are involved in numerous biological processes and diseases, including obesity.The results of these studies in general confirm the trends found by RNAseq.

View Article: PubMed Central - PubMed

Affiliation: Animal Genetics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark; Center for non-coding RNA in Technology and Health, Computational Biology and Bioinformatics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark.

ABSTRACT
Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding to their 3'UTR. They are involved in numerous biological processes and diseases, including obesity. In this study we use a mixed breed pig model designed for obesity studies to investigate differentially expressed miRNAs in subcutaneous adipose tissue by RNA sequencing (RNAseq). Both male and female pigs are included to explore gender differences. The RNAseq study shows that the most highly expressed miRNAs are in accordance with comparable studies in pigs and humans. A total of six miRNAs are differentially expressed in subcutaneous adipose tissue between the lean and obese group of pigs, and in addition gender specific significant differential expression is observed for a number of miRNAs. The differentially expressed miRNAs have been verified using qPCR. The results of these studies in general confirm the trends found by RNAseq. Mir-9 and mir-124a are significantly differentially expressed with large fold changes in subcutaneous adipose tissue between lean and obese pigs. Mir-9 is more highly expressed in the obese pigs with a fold change of 10 and a p-value < 0.001. Mir-124a is more highly expressed in the obese pigs with a fold change of 114 and a p-value < 0.001. In addition, mir-124a is significantly higher expressed in abdominal adipose tissue in male pigs with a fold change of 119 and a p-value < 0.05. Both miRNAs are also significantly higher expressed in the liver of obese male pigs where mir-124a has a fold change of 12 and mir-9 has a fold change of 1.6, both with p-values < 0.05.

No MeSH data available.


Related in: MedlinePlus

Expression of mir-9 and mir-124a in Subcutaneous Adipose Tissue.qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the three microRNAs. P-values calculated by Student’s t-test.
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pone.0131650.g002: Expression of mir-9 and mir-124a in Subcutaneous Adipose Tissue.qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the three microRNAs. P-values calculated by Student’s t-test.

Mentions: The most differentially expressed miRNAs found in the sequencing study together with a selection of potential obesity relevant miRNAs selected from the literature were verified by qPCR (Table 6). Generally the expression pattern followed what was seen in the NGS sequencing data, i.e. a miRNA up regulated in obese pigs in the RNAseq study was also up regulated when analyzed by qPCR. An exception from this general trend was mir-199-5p which was not significantly differentially expressed in the qPCR study. Also mir-489 and mir-34c could not be detected in all animals due to very low expression, and were therefore excluded from the qPCR analysis. Two of the 6 miRNAs found to be differentially expressed in the sequencing study, mir-9 and mir-124a, were significantly differentially expressed between the two groups (Table 6, Fig 2). Both the 3’ and 5’ mature miRNAs of mir-9 were significantly differentially expressed in the sequencing analysis, but only mir-9-5p was tested in qPCR due to low expression of mir-9-3p.


Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs.

Mentzel CM, Anthon C, Jacobsen MJ, Karlskov-Mortensen P, Bruun CS, Jørgensen CB, Gorodkin J, Cirera S, Fredholm M - PLoS ONE (2015)

Expression of mir-9 and mir-124a in Subcutaneous Adipose Tissue.qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the three microRNAs. P-values calculated by Student’s t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519260&req=5

pone.0131650.g002: Expression of mir-9 and mir-124a in Subcutaneous Adipose Tissue.qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). Column scatter plot of the groups Lean and Obese, Lean Male and Obese Male and Lean Female and Obese Female is shown for each of the three microRNAs. P-values calculated by Student’s t-test.
Mentions: The most differentially expressed miRNAs found in the sequencing study together with a selection of potential obesity relevant miRNAs selected from the literature were verified by qPCR (Table 6). Generally the expression pattern followed what was seen in the NGS sequencing data, i.e. a miRNA up regulated in obese pigs in the RNAseq study was also up regulated when analyzed by qPCR. An exception from this general trend was mir-199-5p which was not significantly differentially expressed in the qPCR study. Also mir-489 and mir-34c could not be detected in all animals due to very low expression, and were therefore excluded from the qPCR analysis. Two of the 6 miRNAs found to be differentially expressed in the sequencing study, mir-9 and mir-124a, were significantly differentially expressed between the two groups (Table 6, Fig 2). Both the 3’ and 5’ mature miRNAs of mir-9 were significantly differentially expressed in the sequencing analysis, but only mir-9-5p was tested in qPCR due to low expression of mir-9-3p.

Bottom Line: Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans.They are involved in numerous biological processes and diseases, including obesity.The results of these studies in general confirm the trends found by RNAseq.

View Article: PubMed Central - PubMed

Affiliation: Animal Genetics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark; Center for non-coding RNA in Technology and Health, Computational Biology and Bioinformatics, Department of Veterinary Clinical and Animal Science, Faculty of Health Sciences, University of Copenhagen, Frederiksberg, Denmark.

ABSTRACT
Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding to their 3'UTR. They are involved in numerous biological processes and diseases, including obesity. In this study we use a mixed breed pig model designed for obesity studies to investigate differentially expressed miRNAs in subcutaneous adipose tissue by RNA sequencing (RNAseq). Both male and female pigs are included to explore gender differences. The RNAseq study shows that the most highly expressed miRNAs are in accordance with comparable studies in pigs and humans. A total of six miRNAs are differentially expressed in subcutaneous adipose tissue between the lean and obese group of pigs, and in addition gender specific significant differential expression is observed for a number of miRNAs. The differentially expressed miRNAs have been verified using qPCR. The results of these studies in general confirm the trends found by RNAseq. Mir-9 and mir-124a are significantly differentially expressed with large fold changes in subcutaneous adipose tissue between lean and obese pigs. Mir-9 is more highly expressed in the obese pigs with a fold change of 10 and a p-value < 0.001. Mir-124a is more highly expressed in the obese pigs with a fold change of 114 and a p-value < 0.001. In addition, mir-124a is significantly higher expressed in abdominal adipose tissue in male pigs with a fold change of 119 and a p-value < 0.05. Both miRNAs are also significantly higher expressed in the liver of obese male pigs where mir-124a has a fold change of 12 and mir-9 has a fold change of 1.6, both with p-values < 0.05.

No MeSH data available.


Related in: MedlinePlus