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Clonal Occurrence of Salmonella Weltevreden in Cultured Shrimp in the Mekong Delta, Vietnam.

Noor Uddin GM, Larsen MH, Barco L, Minh Phu T, Dalsgaard A - PLoS ONE (2015)

Bottom Line: Weltevreden was susceptible to most antimicrobials tested, with a few strains being resistant to florfenicol, chloramphenicol, sulfamethoxazole or trimethoprim.Future studies of the ecology of S.Weltevreden should establish if this serovar may survive better and even multiply in warm-water shrimp farm environments compared to other Salmonella serovars.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT
This study investigated the occurrence, serovar and antimicrobial resistance of Salmonella spp. in shrimp samples from intensive and extensive farms located in three different provinces in the Mekong Delta, Vietnam. Shrimp from 11 of the 48 farms all contained S. Weltevreden, except for one farm yielding S. Agona, with no difference in Salmonella occurrence between the two production systems. Pulsed field gel electrophoresis (PFGE) of S. Weltevreden showed closely related XbaI pulse types, suggesting a clonal relationship despite the farms and shrimp samples being epidemiologically unrelated. S. Weltevreden was susceptible to most antimicrobials tested, with a few strains being resistant to florfenicol, chloramphenicol, sulfamethoxazole or trimethoprim. Future studies of the ecology of S. Weltevreden should establish if this serovar may survive better and even multiply in warm-water shrimp farm environments compared to other Salmonella serovars.

No MeSH data available.


XbaI PFGE patterns and antimicrobial susceptibility of Salmonella isolates from shrimp samples from intensive and extensive production system.Similarity analysis was performed using the Dice coefficient, and clustering was done by the unweighted pair-group method with arithmetic averages (UPGMA; position tolerance 1.0%). Antimicrobial resistance was determined using the Sensititre Vizion System and MIC values were evaluated in accordance with the protocol of the Clinical and Laboratory Standards Institute. FFC, Florfenicol; CHL, Chloramphenicol; SMX, Sulfamethoxazole and TMP, Trimethoprim; "S" means susceptible to all antimicrobials tested. MIC values (μg/mL) are shown in brackets.
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pone.0134252.g001: XbaI PFGE patterns and antimicrobial susceptibility of Salmonella isolates from shrimp samples from intensive and extensive production system.Similarity analysis was performed using the Dice coefficient, and clustering was done by the unweighted pair-group method with arithmetic averages (UPGMA; position tolerance 1.0%). Antimicrobial resistance was determined using the Sensititre Vizion System and MIC values were evaluated in accordance with the protocol of the Clinical and Laboratory Standards Institute. FFC, Florfenicol; CHL, Chloramphenicol; SMX, Sulfamethoxazole and TMP, Trimethoprim; "S" means susceptible to all antimicrobials tested. MIC values (μg/mL) are shown in brackets.

Mentions: S. Weltevreden (17 isolates) and S. Agona (1 isolate) representing different PFGE types and at least one isolate per shrimp sample (farm) (Fig 1) were selected for antimicrobial susceptibility testing using Sensititre Vizion System (Trek Diagnostics System, East Grinstead, UK) according to the protocol of the Clinical and Laboratory Standards Institute [28]. The 17 S. Weltevreden isolates selected included two isolates showing a blurred PFGE banding pattern as well as four isolates with an identical pulse type from farm 57 and two isolates with an identical pulse type from farm 13 (Fig 1). The antimicrobial susceptibility of these farm isolates were tested to determine if isolates from the same sample and with identical pulse type showed identical susceptibility patterns. Tests were performed for β-lactam (ampicillin, AMP (0.25 μg/mL); cefotaxime, CTX (8 μg/mL); ceftazidime, CAZ (8 μg/mL)) and non-β-lactam antimicrobials (chloramphenicol, CHL (8 μg/mL); gentamycin, GEN (4 μg/mL); tetracycline, TET (4 μg/mL); sulfamethoxazole, SMX (256 μg/mL); trimethoprim, TMP (2 μg/mL); streptomycin, STR (no standard breakpoints available); colistin, CST (2 μg/mL, for Pseudomonas aeruginosa); florfenicol, FFC (4 μg/mL, for S. choleraesuis); kanamycin, KAN (16 μg/mL); nalidixic acid, NAL (16 μg/mL) and ciprofloxacin, CIP (9.06 μg/mL)). Standard breakpoints for Enterobacteriaceae for most of the antimicrobials except for florfenicol and colistin were used [29]. Standard breakpoints of FFC and CST were used respectively for Salmonella choleraesuis and Pseudomonas aeruginosa [29].


Clonal Occurrence of Salmonella Weltevreden in Cultured Shrimp in the Mekong Delta, Vietnam.

Noor Uddin GM, Larsen MH, Barco L, Minh Phu T, Dalsgaard A - PLoS ONE (2015)

XbaI PFGE patterns and antimicrobial susceptibility of Salmonella isolates from shrimp samples from intensive and extensive production system.Similarity analysis was performed using the Dice coefficient, and clustering was done by the unweighted pair-group method with arithmetic averages (UPGMA; position tolerance 1.0%). Antimicrobial resistance was determined using the Sensititre Vizion System and MIC values were evaluated in accordance with the protocol of the Clinical and Laboratory Standards Institute. FFC, Florfenicol; CHL, Chloramphenicol; SMX, Sulfamethoxazole and TMP, Trimethoprim; "S" means susceptible to all antimicrobials tested. MIC values (μg/mL) are shown in brackets.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519254&req=5

pone.0134252.g001: XbaI PFGE patterns and antimicrobial susceptibility of Salmonella isolates from shrimp samples from intensive and extensive production system.Similarity analysis was performed using the Dice coefficient, and clustering was done by the unweighted pair-group method with arithmetic averages (UPGMA; position tolerance 1.0%). Antimicrobial resistance was determined using the Sensititre Vizion System and MIC values were evaluated in accordance with the protocol of the Clinical and Laboratory Standards Institute. FFC, Florfenicol; CHL, Chloramphenicol; SMX, Sulfamethoxazole and TMP, Trimethoprim; "S" means susceptible to all antimicrobials tested. MIC values (μg/mL) are shown in brackets.
Mentions: S. Weltevreden (17 isolates) and S. Agona (1 isolate) representing different PFGE types and at least one isolate per shrimp sample (farm) (Fig 1) were selected for antimicrobial susceptibility testing using Sensititre Vizion System (Trek Diagnostics System, East Grinstead, UK) according to the protocol of the Clinical and Laboratory Standards Institute [28]. The 17 S. Weltevreden isolates selected included two isolates showing a blurred PFGE banding pattern as well as four isolates with an identical pulse type from farm 57 and two isolates with an identical pulse type from farm 13 (Fig 1). The antimicrobial susceptibility of these farm isolates were tested to determine if isolates from the same sample and with identical pulse type showed identical susceptibility patterns. Tests were performed for β-lactam (ampicillin, AMP (0.25 μg/mL); cefotaxime, CTX (8 μg/mL); ceftazidime, CAZ (8 μg/mL)) and non-β-lactam antimicrobials (chloramphenicol, CHL (8 μg/mL); gentamycin, GEN (4 μg/mL); tetracycline, TET (4 μg/mL); sulfamethoxazole, SMX (256 μg/mL); trimethoprim, TMP (2 μg/mL); streptomycin, STR (no standard breakpoints available); colistin, CST (2 μg/mL, for Pseudomonas aeruginosa); florfenicol, FFC (4 μg/mL, for S. choleraesuis); kanamycin, KAN (16 μg/mL); nalidixic acid, NAL (16 μg/mL) and ciprofloxacin, CIP (9.06 μg/mL)). Standard breakpoints for Enterobacteriaceae for most of the antimicrobials except for florfenicol and colistin were used [29]. Standard breakpoints of FFC and CST were used respectively for Salmonella choleraesuis and Pseudomonas aeruginosa [29].

Bottom Line: Weltevreden was susceptible to most antimicrobials tested, with a few strains being resistant to florfenicol, chloramphenicol, sulfamethoxazole or trimethoprim.Future studies of the ecology of S.Weltevreden should establish if this serovar may survive better and even multiply in warm-water shrimp farm environments compared to other Salmonella serovars.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT
This study investigated the occurrence, serovar and antimicrobial resistance of Salmonella spp. in shrimp samples from intensive and extensive farms located in three different provinces in the Mekong Delta, Vietnam. Shrimp from 11 of the 48 farms all contained S. Weltevreden, except for one farm yielding S. Agona, with no difference in Salmonella occurrence between the two production systems. Pulsed field gel electrophoresis (PFGE) of S. Weltevreden showed closely related XbaI pulse types, suggesting a clonal relationship despite the farms and shrimp samples being epidemiologically unrelated. S. Weltevreden was susceptible to most antimicrobials tested, with a few strains being resistant to florfenicol, chloramphenicol, sulfamethoxazole or trimethoprim. Future studies of the ecology of S. Weltevreden should establish if this serovar may survive better and even multiply in warm-water shrimp farm environments compared to other Salmonella serovars.

No MeSH data available.