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Microbial Population Differentials between Mucosal and Submucosal Intestinal Tissues in Advanced Crohn's Disease of the Ileum.

Chiodini RJ, Dowd SE, Chamberlin WM, Galandiuk S, Davis B, Glassing A - PLoS ONE (2015)

Bottom Line: A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp.In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp.Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

View Article: PubMed Central - PubMed

Affiliation: St. Vincent Healthcare, Sisters of Charity of Leavenworth Health System, Billings, Montana, United States of America; Department of Biological and Physical Sciences, Montana State University-Billings, Billings, Montana, United States of America.

ABSTRACT
Since Crohn's disease is a transmural disease, we hypothesized that examination of deep submucosal tissues directly involved in the inflammatory disease process may provide unique insights into bacterial populations transgressing intestinal barriers and bacterial populations more representative of the causes and agents of the disease. We performed deep 16s microbiota sequencing on isolated ilea mucosal and submucosal tissues on 20 patients with Crohn's disease and 15 non-inflammatory bowel disease controls with a depth of coverage averaging 81,500 sequences in each of the 70 DNA samples yielding an overall resolution down to 0.0001% of the bacterial population. Of the 4,802,328 total sequences generated, 98.9% or 4,749,183 sequences aligned with the Kingdom Bacteria that clustered into 8545 unique sequences with <3% divergence or operational taxonomic units enabling the identification of 401 genera and 698 tentative bacterial species. There were significant differences in all taxonomic levels between the submucosal microbiota in Crohn's disease compared to controls, including organisms of the Order Desulfovibrionales that were present within the submucosal tissues of most Crohn's disease patients but absent in the control group. A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp. In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp. This is the first study to examine the microbial populations within submucosal tissues of patients with Crohn's disease and to compare microbial communities found deep within the submucosal tissues with those present on mucosal surfaces. Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

No MeSH data available.


Related in: MedlinePlus

Bacterial populations of the diseased submucosa and mucosa at the center of the diseased tissues in Crohn's disease.The values in parentheses represent the average relative frequency in mucosa/submucosa. An asterisk (*) denotes that the difference between the mucosa and submucosa was statistically significant (p = <0.05) while a plus (+) represents a trend toward statistical significance (p = >0.05 but <0.065).
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pone.0134382.g005: Bacterial populations of the diseased submucosa and mucosa at the center of the diseased tissues in Crohn's disease.The values in parentheses represent the average relative frequency in mucosa/submucosa. An asterisk (*) denotes that the difference between the mucosa and submucosa was statistically significant (p = <0.05) while a plus (+) represents a trend toward statistical significance (p = >0.05 but <0.065).

Mentions: The predominate organisms that accounted for 95% of the relative bacterial populations of the diseased mucosa and submucosa are illustrated in Fig 5.


Microbial Population Differentials between Mucosal and Submucosal Intestinal Tissues in Advanced Crohn's Disease of the Ileum.

Chiodini RJ, Dowd SE, Chamberlin WM, Galandiuk S, Davis B, Glassing A - PLoS ONE (2015)

Bacterial populations of the diseased submucosa and mucosa at the center of the diseased tissues in Crohn's disease.The values in parentheses represent the average relative frequency in mucosa/submucosa. An asterisk (*) denotes that the difference between the mucosa and submucosa was statistically significant (p = <0.05) while a plus (+) represents a trend toward statistical significance (p = >0.05 but <0.065).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519195&req=5

pone.0134382.g005: Bacterial populations of the diseased submucosa and mucosa at the center of the diseased tissues in Crohn's disease.The values in parentheses represent the average relative frequency in mucosa/submucosa. An asterisk (*) denotes that the difference between the mucosa and submucosa was statistically significant (p = <0.05) while a plus (+) represents a trend toward statistical significance (p = >0.05 but <0.065).
Mentions: The predominate organisms that accounted for 95% of the relative bacterial populations of the diseased mucosa and submucosa are illustrated in Fig 5.

Bottom Line: A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp.In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp.Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

View Article: PubMed Central - PubMed

Affiliation: St. Vincent Healthcare, Sisters of Charity of Leavenworth Health System, Billings, Montana, United States of America; Department of Biological and Physical Sciences, Montana State University-Billings, Billings, Montana, United States of America.

ABSTRACT
Since Crohn's disease is a transmural disease, we hypothesized that examination of deep submucosal tissues directly involved in the inflammatory disease process may provide unique insights into bacterial populations transgressing intestinal barriers and bacterial populations more representative of the causes and agents of the disease. We performed deep 16s microbiota sequencing on isolated ilea mucosal and submucosal tissues on 20 patients with Crohn's disease and 15 non-inflammatory bowel disease controls with a depth of coverage averaging 81,500 sequences in each of the 70 DNA samples yielding an overall resolution down to 0.0001% of the bacterial population. Of the 4,802,328 total sequences generated, 98.9% or 4,749,183 sequences aligned with the Kingdom Bacteria that clustered into 8545 unique sequences with <3% divergence or operational taxonomic units enabling the identification of 401 genera and 698 tentative bacterial species. There were significant differences in all taxonomic levels between the submucosal microbiota in Crohn's disease compared to controls, including organisms of the Order Desulfovibrionales that were present within the submucosal tissues of most Crohn's disease patients but absent in the control group. A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp. In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp. This is the first study to examine the microbial populations within submucosal tissues of patients with Crohn's disease and to compare microbial communities found deep within the submucosal tissues with those present on mucosal surfaces. Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

No MeSH data available.


Related in: MedlinePlus