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Microbial Population Differentials between Mucosal and Submucosal Intestinal Tissues in Advanced Crohn's Disease of the Ileum.

Chiodini RJ, Dowd SE, Chamberlin WM, Galandiuk S, Davis B, Glassing A - PLoS ONE (2015)

Bottom Line: A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp.In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp.Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

View Article: PubMed Central - PubMed

Affiliation: St. Vincent Healthcare, Sisters of Charity of Leavenworth Health System, Billings, Montana, United States of America; Department of Biological and Physical Sciences, Montana State University-Billings, Billings, Montana, United States of America.

ABSTRACT
Since Crohn's disease is a transmural disease, we hypothesized that examination of deep submucosal tissues directly involved in the inflammatory disease process may provide unique insights into bacterial populations transgressing intestinal barriers and bacterial populations more representative of the causes and agents of the disease. We performed deep 16s microbiota sequencing on isolated ilea mucosal and submucosal tissues on 20 patients with Crohn's disease and 15 non-inflammatory bowel disease controls with a depth of coverage averaging 81,500 sequences in each of the 70 DNA samples yielding an overall resolution down to 0.0001% of the bacterial population. Of the 4,802,328 total sequences generated, 98.9% or 4,749,183 sequences aligned with the Kingdom Bacteria that clustered into 8545 unique sequences with <3% divergence or operational taxonomic units enabling the identification of 401 genera and 698 tentative bacterial species. There were significant differences in all taxonomic levels between the submucosal microbiota in Crohn's disease compared to controls, including organisms of the Order Desulfovibrionales that were present within the submucosal tissues of most Crohn's disease patients but absent in the control group. A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp. In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp. This is the first study to examine the microbial populations within submucosal tissues of patients with Crohn's disease and to compare microbial communities found deep within the submucosal tissues with those present on mucosal surfaces. Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

No MeSH data available.


Related in: MedlinePlus

Differences in the relative abundance of bacterial Orders (A) and Families (B) in the mucosa of patients with ileal Crohn's disease compared to the ileal mucosa in nIBD controls.Except for an increase in bacteria of the Order Bacteroidales and Enterobacteriales, mucosa from ileal Crohn's disease patients had a reduced abundance of most other bacteria. There were major differences in most bacterial Families between Crohn's disease and controls. Illustrated are only bacteria comprising > 1% of the total bacterial population and present in >50% of patients.
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pone.0134382.g004: Differences in the relative abundance of bacterial Orders (A) and Families (B) in the mucosa of patients with ileal Crohn's disease compared to the ileal mucosa in nIBD controls.Except for an increase in bacteria of the Order Bacteroidales and Enterobacteriales, mucosa from ileal Crohn's disease patients had a reduced abundance of most other bacteria. There were major differences in most bacterial Families between Crohn's disease and controls. Illustrated are only bacteria comprising > 1% of the total bacterial population and present in >50% of patients.

Mentions: The relative abundance of bacterial populations in the mucosa of patients with Crohn's disease were significantly different from that found in controls, particularly farther down the phylogenic tree (Fig 4). At the Phylum level, only Bacteroidetes were significantly increased (p = 0.002) and organisms within this Phylum remained a statistically significant population down to the family level. These findings only loosely correlated with those reported in other studies. Most studies have reported decreases in organisms of the Phylum Firmicutes with accompanying increases in Proteobacteria and Bacteroidetes in Crohn's disease [2,19]. Although Firmicutes were reduced and Proteobacteria were increased in Crohn's disease mucosa, they were not significantly different from nIBD control mucosa (p >0.05).


Microbial Population Differentials between Mucosal and Submucosal Intestinal Tissues in Advanced Crohn's Disease of the Ileum.

Chiodini RJ, Dowd SE, Chamberlin WM, Galandiuk S, Davis B, Glassing A - PLoS ONE (2015)

Differences in the relative abundance of bacterial Orders (A) and Families (B) in the mucosa of patients with ileal Crohn's disease compared to the ileal mucosa in nIBD controls.Except for an increase in bacteria of the Order Bacteroidales and Enterobacteriales, mucosa from ileal Crohn's disease patients had a reduced abundance of most other bacteria. There were major differences in most bacterial Families between Crohn's disease and controls. Illustrated are only bacteria comprising > 1% of the total bacterial population and present in >50% of patients.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519195&req=5

pone.0134382.g004: Differences in the relative abundance of bacterial Orders (A) and Families (B) in the mucosa of patients with ileal Crohn's disease compared to the ileal mucosa in nIBD controls.Except for an increase in bacteria of the Order Bacteroidales and Enterobacteriales, mucosa from ileal Crohn's disease patients had a reduced abundance of most other bacteria. There were major differences in most bacterial Families between Crohn's disease and controls. Illustrated are only bacteria comprising > 1% of the total bacterial population and present in >50% of patients.
Mentions: The relative abundance of bacterial populations in the mucosa of patients with Crohn's disease were significantly different from that found in controls, particularly farther down the phylogenic tree (Fig 4). At the Phylum level, only Bacteroidetes were significantly increased (p = 0.002) and organisms within this Phylum remained a statistically significant population down to the family level. These findings only loosely correlated with those reported in other studies. Most studies have reported decreases in organisms of the Phylum Firmicutes with accompanying increases in Proteobacteria and Bacteroidetes in Crohn's disease [2,19]. Although Firmicutes were reduced and Proteobacteria were increased in Crohn's disease mucosa, they were not significantly different from nIBD control mucosa (p >0.05).

Bottom Line: A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp.In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp.Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

View Article: PubMed Central - PubMed

Affiliation: St. Vincent Healthcare, Sisters of Charity of Leavenworth Health System, Billings, Montana, United States of America; Department of Biological and Physical Sciences, Montana State University-Billings, Billings, Montana, United States of America.

ABSTRACT
Since Crohn's disease is a transmural disease, we hypothesized that examination of deep submucosal tissues directly involved in the inflammatory disease process may provide unique insights into bacterial populations transgressing intestinal barriers and bacterial populations more representative of the causes and agents of the disease. We performed deep 16s microbiota sequencing on isolated ilea mucosal and submucosal tissues on 20 patients with Crohn's disease and 15 non-inflammatory bowel disease controls with a depth of coverage averaging 81,500 sequences in each of the 70 DNA samples yielding an overall resolution down to 0.0001% of the bacterial population. Of the 4,802,328 total sequences generated, 98.9% or 4,749,183 sequences aligned with the Kingdom Bacteria that clustered into 8545 unique sequences with <3% divergence or operational taxonomic units enabling the identification of 401 genera and 698 tentative bacterial species. There were significant differences in all taxonomic levels between the submucosal microbiota in Crohn's disease compared to controls, including organisms of the Order Desulfovibrionales that were present within the submucosal tissues of most Crohn's disease patients but absent in the control group. A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp. In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp. This is the first study to examine the microbial populations within submucosal tissues of patients with Crohn's disease and to compare microbial communities found deep within the submucosal tissues with those present on mucosal surfaces. Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

No MeSH data available.


Related in: MedlinePlus