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Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Morita H, Obata K, Abe C, Shiba D, Shirakawa M, Kudo T, Takahashi S - PLoS ONE (2015)

Bottom Line: To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space.Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge.Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Gifu University Graduate School of Medicine, Gifu, Japan; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency, Tsukuba, Japan.

ABSTRACT
To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

No MeSH data available.


Related in: MedlinePlus

Summarized data for the daily changes in body mass during the 2-week 1.4 g load in the Sham (top) and the VL (bottom) mice.* P < 0.05 vs. Int-1g.
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pone.0133981.g007: Summarized data for the daily changes in body mass during the 2-week 1.4 g load in the Sham (top) and the VL (bottom) mice.* P < 0.05 vs. Int-1g.

Mentions: To examine the role of the vestibular system on hypergravity-induced reductions in body mass, this variable was measured for 2 weeks in the Sham and VL mice in a 1 g or 1.4 g environment (Fig 7). The body masses immediately before the experiment were significantly smaller in the VL mice than those in the Sham mice (Table 2). In the Sham mice, there was a significant interaction between group and time [F(2,28) = 6.094, P < 0.001]. The body masses of the Sham-1.4g-S and the Sham-1.4g-L were significantly different from those in the Sham-1g, but no difference was found between the Sham-1.4g-S and the Sham-1.4g-L (P = 0.529). Furthermore, no interaction between group and time was found in the VL mice [F(2,28) = 0.558, P = 0.471]. That is, the suppressive effect of hypergravity on body mass growth was abolished by VL.


Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Morita H, Obata K, Abe C, Shiba D, Shirakawa M, Kudo T, Takahashi S - PLoS ONE (2015)

Summarized data for the daily changes in body mass during the 2-week 1.4 g load in the Sham (top) and the VL (bottom) mice.* P < 0.05 vs. Int-1g.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519191&req=5

pone.0133981.g007: Summarized data for the daily changes in body mass during the 2-week 1.4 g load in the Sham (top) and the VL (bottom) mice.* P < 0.05 vs. Int-1g.
Mentions: To examine the role of the vestibular system on hypergravity-induced reductions in body mass, this variable was measured for 2 weeks in the Sham and VL mice in a 1 g or 1.4 g environment (Fig 7). The body masses immediately before the experiment were significantly smaller in the VL mice than those in the Sham mice (Table 2). In the Sham mice, there was a significant interaction between group and time [F(2,28) = 6.094, P < 0.001]. The body masses of the Sham-1.4g-S and the Sham-1.4g-L were significantly different from those in the Sham-1g, but no difference was found between the Sham-1.4g-S and the Sham-1.4g-L (P = 0.529). Furthermore, no interaction between group and time was found in the VL mice [F(2,28) = 0.558, P = 0.471]. That is, the suppressive effect of hypergravity on body mass growth was abolished by VL.

Bottom Line: To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space.Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge.Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Gifu University Graduate School of Medicine, Gifu, Japan; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency, Tsukuba, Japan.

ABSTRACT
To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

No MeSH data available.


Related in: MedlinePlus