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Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Morita H, Obata K, Abe C, Shiba D, Shirakawa M, Kudo T, Takahashi S - PLoS ONE (2015)

Bottom Line: To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space.Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge.Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Gifu University Graduate School of Medicine, Gifu, Japan; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency, Tsukuba, Japan.

ABSTRACT
To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

No MeSH data available.


Related in: MedlinePlus

Posture at the onset of 2 g in a Sham (A) and a VL (B) mouse.
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pone.0133981.g005: Posture at the onset of 2 g in a Sham (A) and a VL (B) mouse.

Mentions: Behavioral analysis revealed that the round shape of the lumbar was lost, the trunk became flat, and the tail went up in all Sham-2g-S mice (Fig 5A and S2 File). This characteristic posture was not seen in the VL-2g-S mice, although they looked like they felt something and became somewhat restless at the onset of acceleration (Fig 5B and S3 File). Furthermore, all Sham-2g-S mice remained still and did not move during the 90-min 2 g period; however, VL-2g-S mice moved and sometimes reared up.


Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Morita H, Obata K, Abe C, Shiba D, Shirakawa M, Kudo T, Takahashi S - PLoS ONE (2015)

Posture at the onset of 2 g in a Sham (A) and a VL (B) mouse.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519191&req=5

pone.0133981.g005: Posture at the onset of 2 g in a Sham (A) and a VL (B) mouse.
Mentions: Behavioral analysis revealed that the round shape of the lumbar was lost, the trunk became flat, and the tail went up in all Sham-2g-S mice (Fig 5A and S2 File). This characteristic posture was not seen in the VL-2g-S mice, although they looked like they felt something and became somewhat restless at the onset of acceleration (Fig 5B and S3 File). Furthermore, all Sham-2g-S mice remained still and did not move during the 90-min 2 g period; however, VL-2g-S mice moved and sometimes reared up.

Bottom Line: To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space.Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge.Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Gifu University Graduate School of Medicine, Gifu, Japan; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency, Tsukuba, Japan.

ABSTRACT
To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

No MeSH data available.


Related in: MedlinePlus