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Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Morita H, Obata K, Abe C, Shiba D, Shirakawa M, Kudo T, Takahashi S - PLoS ONE (2015)

Bottom Line: To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space.Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge.Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Gifu University Graduate School of Medicine, Gifu, Japan; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency, Tsukuba, Japan.

ABSTRACT
To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

No MeSH data available.


Related in: MedlinePlus

Photographs and a diagram of the newly-developed gondola-type short-arm centrifuge, which is equipped with 4 gondolas.Each gondola is equipped with a white LED, an infrared LED, a video camera for monitoring the behavior of mice, and an air fan for refreshing the air.
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pone.0133981.g001: Photographs and a diagram of the newly-developed gondola-type short-arm centrifuge, which is equipped with 4 gondolas.Each gondola is equipped with a white LED, an infrared LED, a video camera for monitoring the behavior of mice, and an air fan for refreshing the air.

Mentions: The short-arm centrifuge is designed so that the radius of gyration at the center of the individual cage becomes 15 cm (Fig 1). Thus, centrifugation at 77 rpm produces a centrifugal force of 1 g, and this force acts in the dorsoventral direction of a mouse under the microgravity environment in space (Fig 2A). The radius of gyration shortens if the mouse rears up, and the centrifugal force at the head becomes small, because the centrifugal force is proportional to the radius of gyration. For example, if the head of a mouse is 5 cm above the base, the centrifugal force at the head becomes 0.66 g; this effect is negligible (0.97 g) if the long-arm (150 cm) centrifuge is used.


Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Morita H, Obata K, Abe C, Shiba D, Shirakawa M, Kudo T, Takahashi S - PLoS ONE (2015)

Photographs and a diagram of the newly-developed gondola-type short-arm centrifuge, which is equipped with 4 gondolas.Each gondola is equipped with a white LED, an infrared LED, a video camera for monitoring the behavior of mice, and an air fan for refreshing the air.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4519191&req=5

pone.0133981.g001: Photographs and a diagram of the newly-developed gondola-type short-arm centrifuge, which is equipped with 4 gondolas.Each gondola is equipped with a white LED, an infrared LED, a video camera for monitoring the behavior of mice, and an air fan for refreshing the air.
Mentions: The short-arm centrifuge is designed so that the radius of gyration at the center of the individual cage becomes 15 cm (Fig 1). Thus, centrifugation at 77 rpm produces a centrifugal force of 1 g, and this force acts in the dorsoventral direction of a mouse under the microgravity environment in space (Fig 2A). The radius of gyration shortens if the mouse rears up, and the centrifugal force at the head becomes small, because the centrifugal force is proportional to the radius of gyration. For example, if the head of a mouse is 5 cm above the base, the centrifugal force at the head becomes 0.66 g; this effect is negligible (0.97 g) if the long-arm (150 cm) centrifuge is used.

Bottom Line: To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space.Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge.Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Gifu University Graduate School of Medicine, Gifu, Japan; Mouse Epigenetics Project, ISS/Kibo experiment, Japan Aerospace Exploration Agency, Tsukuba, Japan.

ABSTRACT
To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

No MeSH data available.


Related in: MedlinePlus