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Strong Manual Acupuncture Manipulation Could Better Inhibit Spike Frequency of the Dorsal Horn Neurons in Rats with Acute Visceral Nociception.

Hong S, Ding S, Wu F, Xi Q, Li Q, Liu Y, Zhou T, Qing C, Guo Y, Guo Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Here, we analyzed the spike frequency of excitatory gastric-related wide dynamic range (WDR) neurons in spinal dorsal horn (SDH) following acute gastric distension (GD) in rats and compared their responses to MA manipulations with four different frequencies (0.5, 1, 2, and 3 Hz) at Zusanli (ST36).Our previous researches had demonstrated that different MA manipulations could increase spike frequency in an intensity-dependent manner in normal rats; these findings suggest that acupuncture may have different modulatory effects depending on the state of the stomach.Since neuronal spike frequency was related to the level of nociception, the results suggest that strong MA manipulation may have better effect on acute visceral nociception.

View Article: PubMed Central - PubMed

Affiliation: Acupuncture Department, Zhejiang Provincial Hospital of TCM, No. 54, Youdian Road, Hangzhou 310006, China ; Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.

ABSTRACT
Afferent impulses from visceral nociception can be regulated by acupuncture at spinal cord level; however, the effects of different manual acupuncture (MA) manipulations on the afferent impulses are still unknown. Here, we analyzed the spike frequency of excitatory gastric-related wide dynamic range (WDR) neurons in spinal dorsal horn (SDH) following acute gastric distension (GD) in rats and compared their responses to MA manipulations with four different frequencies (0.5, 1, 2, and 3 Hz) at Zusanli (ST36). Results indicated that the spike frequency was increased by acute GD stimulation. Under acute GD circumstances, the spike frequency was further activated by weak MA stimulation (0.5 and 1 Hz), while being significantly inhibited by strong MA stimulation (2 and 3 Hz). After 10 minutes of the strong MA stimulation, same intensity of acute GD caused less spike frequency. Our previous researches had demonstrated that different MA manipulations could increase spike frequency in an intensity-dependent manner in normal rats; these findings suggest that acupuncture may have different modulatory effects depending on the state of the stomach. Since neuronal spike frequency was related to the level of nociception, the results suggest that strong MA manipulation may have better effect on acute visceral nociception.

No MeSH data available.


Related in: MedlinePlus

Different effects of GD on WDR neurons in SDH. 87 WDR neurons recorded in SDH. There were 38 GD nonrelated neurons (44%), 35 GD excited neurons (40%), and 14 GD inhibited neurons (16%).
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fig3: Different effects of GD on WDR neurons in SDH. 87 WDR neurons recorded in SDH. There were 38 GD nonrelated neurons (44%), 35 GD excited neurons (40%), and 14 GD inhibited neurons (16%).

Mentions: Of all the 87 WDR neurons (most of the WDR neurons showed background activities in the absence of stimulation) recorded in SDH after gastric distention, 49 neurons were sensitized by the stimulation. Amongst them, 35 (40%) were excited and 14 (16%) were inhibited by GD (Figure 3). They showed more than 15% change (change rate = the number of spikes after stimulation/the number of spikes before stimulation) in the number of spikes and were defined as gastric-related neurons [23]. In the experiment, we focused on the excitatory gastric-related neurons. At the same time, those neurons were sensitized by MA at ST36. In Figure 4, examples of the extracellular activity of excitatory gastric-related WDR neurons in SDH were evoked by GD and (or) MA with different frequencies at ST36.


Strong Manual Acupuncture Manipulation Could Better Inhibit Spike Frequency of the Dorsal Horn Neurons in Rats with Acute Visceral Nociception.

Hong S, Ding S, Wu F, Xi Q, Li Q, Liu Y, Zhou T, Qing C, Guo Y, Guo Y - Evid Based Complement Alternat Med (2015)

Different effects of GD on WDR neurons in SDH. 87 WDR neurons recorded in SDH. There were 38 GD nonrelated neurons (44%), 35 GD excited neurons (40%), and 14 GD inhibited neurons (16%).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4518182&req=5

fig3: Different effects of GD on WDR neurons in SDH. 87 WDR neurons recorded in SDH. There were 38 GD nonrelated neurons (44%), 35 GD excited neurons (40%), and 14 GD inhibited neurons (16%).
Mentions: Of all the 87 WDR neurons (most of the WDR neurons showed background activities in the absence of stimulation) recorded in SDH after gastric distention, 49 neurons were sensitized by the stimulation. Amongst them, 35 (40%) were excited and 14 (16%) were inhibited by GD (Figure 3). They showed more than 15% change (change rate = the number of spikes after stimulation/the number of spikes before stimulation) in the number of spikes and were defined as gastric-related neurons [23]. In the experiment, we focused on the excitatory gastric-related neurons. At the same time, those neurons were sensitized by MA at ST36. In Figure 4, examples of the extracellular activity of excitatory gastric-related WDR neurons in SDH were evoked by GD and (or) MA with different frequencies at ST36.

Bottom Line: Here, we analyzed the spike frequency of excitatory gastric-related wide dynamic range (WDR) neurons in spinal dorsal horn (SDH) following acute gastric distension (GD) in rats and compared their responses to MA manipulations with four different frequencies (0.5, 1, 2, and 3 Hz) at Zusanli (ST36).Our previous researches had demonstrated that different MA manipulations could increase spike frequency in an intensity-dependent manner in normal rats; these findings suggest that acupuncture may have different modulatory effects depending on the state of the stomach.Since neuronal spike frequency was related to the level of nociception, the results suggest that strong MA manipulation may have better effect on acute visceral nociception.

View Article: PubMed Central - PubMed

Affiliation: Acupuncture Department, Zhejiang Provincial Hospital of TCM, No. 54, Youdian Road, Hangzhou 310006, China ; Acupuncture, Moxibustion and Tuina College, Tianjin University of Traditional Chinese Medicine, No. 312, Anshan West Road, Nankai District, Tianjin 300193, China.

ABSTRACT
Afferent impulses from visceral nociception can be regulated by acupuncture at spinal cord level; however, the effects of different manual acupuncture (MA) manipulations on the afferent impulses are still unknown. Here, we analyzed the spike frequency of excitatory gastric-related wide dynamic range (WDR) neurons in spinal dorsal horn (SDH) following acute gastric distension (GD) in rats and compared their responses to MA manipulations with four different frequencies (0.5, 1, 2, and 3 Hz) at Zusanli (ST36). Results indicated that the spike frequency was increased by acute GD stimulation. Under acute GD circumstances, the spike frequency was further activated by weak MA stimulation (0.5 and 1 Hz), while being significantly inhibited by strong MA stimulation (2 and 3 Hz). After 10 minutes of the strong MA stimulation, same intensity of acute GD caused less spike frequency. Our previous researches had demonstrated that different MA manipulations could increase spike frequency in an intensity-dependent manner in normal rats; these findings suggest that acupuncture may have different modulatory effects depending on the state of the stomach. Since neuronal spike frequency was related to the level of nociception, the results suggest that strong MA manipulation may have better effect on acute visceral nociception.

No MeSH data available.


Related in: MedlinePlus