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A prospective study of macrophage migration inhibitory factor as a marker of inflammatory detection.

Zhang C, Hou G, Liang T, Song J, Qu L, Chen F, Xu J, Wang D, Han J - J. Cell. Mol. Med. (2008)

Bottom Line: MIF mRNA expression was threefold increased in inflammatory tissues at 24 hrs compared with normal tissues, and twofold increased at 48 hrs.MIF protein expression was stronger in the inflammatory tissues at 48 hrs after focal inflammation occurred.Our findings suggest that the (131)I-labelled anti-MIF McAb appears to be more specific and suitable than (131)I-labelled IgG for targeting focal inflammation, which means MIF can be used as a better marker of inflammatory detection.

View Article: PubMed Central - PubMed

Affiliation: Department of Nuclear Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, PR China.

ABSTRACT
This study was to evaluate whether macrophage migration inhibitory factor (MIF) can be used as a better marker of inflammatory detection through the biodistribution and inflammatory imaging study with (131)I-labelled anti-MIF McAb and control antibody in inflammatory model mice. The mRNA and protein expression of MIF in inflammatory lesions were proved by RT-PCR and immunohistochemistry. The model mice were injected with 3.7 MBq of each agent and killed at 24, 48 and 72 hrs after injection. Whole-body images were obtained with storage phosphor screen. The organs, blood, abscesses muscles were removed, weighed and counted with a gamma counter. The percentage of uptake by organs and per gram tissues and abscess/normal tissue (%ID/g) concentration ratios were calculated. The abscesses in mice were well visualized from 24 hrs. The target-to-non-target (T/NT) ratios were 6.71 +/- 1.09 (24 hrs), 8.57 +/- 0.81 (48 hrs) and 11.41 +/- 0.37 (72 hrs) for (131)I-labelled anti-MIF McAb group; while in control group of (131)I-IgG, T/NT ratios were 4.65 +/- 0.63 (24 hrs), 6.44 +/- 0.60 (48 hrs) and 8.23 +/- 0.35 (72 hrs) (P < 0.05). MIF mRNA expression was threefold increased in inflammatory tissues at 24 hrs compared with normal tissues, and twofold increased at 48 hrs. MIF protein expression was stronger in the inflammatory tissues at 48 hrs after focal inflammation occurred. Our findings suggest that the (131)I-labelled anti-MIF McAb appears to be more specific and suitable than (131)I-labelled IgG for targeting focal inflammation, which means MIF can be used as a better marker of inflammatory detection.

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Related in: MedlinePlus

The expression of MIF mRNA in inflammatory and normal tissues. There were little changes in MIF gene expression in normal tissues at three time-points. There was a threefold increase in MIF mRNA expression in inflammatory tissues at 24 hrs compared with normal tissues (P < 0.05). There was a twofold increase in MIF mRNA levels in inflammatory tissues at 48 hrs compared with normal tissues (P < 0.05). Semi-quantitative RT-PCR was performed in duplicate to minimize experimental error on the value calculated. All columnar values were expressed as means and standard deviations. A pattern of results were analysed by repeating at least three times. P < 0.05 compared with the normal group.
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fig03: The expression of MIF mRNA in inflammatory and normal tissues. There were little changes in MIF gene expression in normal tissues at three time-points. There was a threefold increase in MIF mRNA expression in inflammatory tissues at 24 hrs compared with normal tissues (P < 0.05). There was a twofold increase in MIF mRNA levels in inflammatory tissues at 48 hrs compared with normal tissues (P < 0.05). Semi-quantitative RT-PCR was performed in duplicate to minimize experimental error on the value calculated. All columnar values were expressed as means and standard deviations. A pattern of results were analysed by repeating at least three times. P < 0.05 compared with the normal group.

Mentions: In order to make sure that the high intake of 131I-labelled anti-MIF McAb in inflammatory tissue is caused by high expression of MIF in locus, we analysed the expression of MIF in inflammatory tissue. Result of RT-PCR showed the expression of MIF mRNA in inflammatory and normal tissues in Figure 3. There were little changes in MIF gene expression in normal tissues at the three time-points. However, there was a threefold increase in MIF mRNA expression in inflammatory tissues at 24 hrs compared with normal tissues (P < 0.05). There was a twofold increase in MIF mRNA levels in inflammatory tissues at 48 hrs compared with normal tissues (P < 0.05). MIF is secreted by the activated lymphocytes and macrophages and inhibit the migration of macrophages [19, 20], which may lead to the accumulation and activation of macrophages. These activated macrophages may produce high levels of inflammatory MIF seen in inflammatory pathological conditions.


A prospective study of macrophage migration inhibitory factor as a marker of inflammatory detection.

Zhang C, Hou G, Liang T, Song J, Qu L, Chen F, Xu J, Wang D, Han J - J. Cell. Mol. Med. (2008)

The expression of MIF mRNA in inflammatory and normal tissues. There were little changes in MIF gene expression in normal tissues at three time-points. There was a threefold increase in MIF mRNA expression in inflammatory tissues at 24 hrs compared with normal tissues (P < 0.05). There was a twofold increase in MIF mRNA levels in inflammatory tissues at 48 hrs compared with normal tissues (P < 0.05). Semi-quantitative RT-PCR was performed in duplicate to minimize experimental error on the value calculated. All columnar values were expressed as means and standard deviations. A pattern of results were analysed by repeating at least three times. P < 0.05 compared with the normal group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4516554&req=5

fig03: The expression of MIF mRNA in inflammatory and normal tissues. There were little changes in MIF gene expression in normal tissues at three time-points. There was a threefold increase in MIF mRNA expression in inflammatory tissues at 24 hrs compared with normal tissues (P < 0.05). There was a twofold increase in MIF mRNA levels in inflammatory tissues at 48 hrs compared with normal tissues (P < 0.05). Semi-quantitative RT-PCR was performed in duplicate to minimize experimental error on the value calculated. All columnar values were expressed as means and standard deviations. A pattern of results were analysed by repeating at least three times. P < 0.05 compared with the normal group.
Mentions: In order to make sure that the high intake of 131I-labelled anti-MIF McAb in inflammatory tissue is caused by high expression of MIF in locus, we analysed the expression of MIF in inflammatory tissue. Result of RT-PCR showed the expression of MIF mRNA in inflammatory and normal tissues in Figure 3. There were little changes in MIF gene expression in normal tissues at the three time-points. However, there was a threefold increase in MIF mRNA expression in inflammatory tissues at 24 hrs compared with normal tissues (P < 0.05). There was a twofold increase in MIF mRNA levels in inflammatory tissues at 48 hrs compared with normal tissues (P < 0.05). MIF is secreted by the activated lymphocytes and macrophages and inhibit the migration of macrophages [19, 20], which may lead to the accumulation and activation of macrophages. These activated macrophages may produce high levels of inflammatory MIF seen in inflammatory pathological conditions.

Bottom Line: MIF mRNA expression was threefold increased in inflammatory tissues at 24 hrs compared with normal tissues, and twofold increased at 48 hrs.MIF protein expression was stronger in the inflammatory tissues at 48 hrs after focal inflammation occurred.Our findings suggest that the (131)I-labelled anti-MIF McAb appears to be more specific and suitable than (131)I-labelled IgG for targeting focal inflammation, which means MIF can be used as a better marker of inflammatory detection.

View Article: PubMed Central - PubMed

Affiliation: Department of Nuclear Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, PR China.

ABSTRACT
This study was to evaluate whether macrophage migration inhibitory factor (MIF) can be used as a better marker of inflammatory detection through the biodistribution and inflammatory imaging study with (131)I-labelled anti-MIF McAb and control antibody in inflammatory model mice. The mRNA and protein expression of MIF in inflammatory lesions were proved by RT-PCR and immunohistochemistry. The model mice were injected with 3.7 MBq of each agent and killed at 24, 48 and 72 hrs after injection. Whole-body images were obtained with storage phosphor screen. The organs, blood, abscesses muscles were removed, weighed and counted with a gamma counter. The percentage of uptake by organs and per gram tissues and abscess/normal tissue (%ID/g) concentration ratios were calculated. The abscesses in mice were well visualized from 24 hrs. The target-to-non-target (T/NT) ratios were 6.71 +/- 1.09 (24 hrs), 8.57 +/- 0.81 (48 hrs) and 11.41 +/- 0.37 (72 hrs) for (131)I-labelled anti-MIF McAb group; while in control group of (131)I-IgG, T/NT ratios were 4.65 +/- 0.63 (24 hrs), 6.44 +/- 0.60 (48 hrs) and 8.23 +/- 0.35 (72 hrs) (P < 0.05). MIF mRNA expression was threefold increased in inflammatory tissues at 24 hrs compared with normal tissues, and twofold increased at 48 hrs. MIF protein expression was stronger in the inflammatory tissues at 48 hrs after focal inflammation occurred. Our findings suggest that the (131)I-labelled anti-MIF McAb appears to be more specific and suitable than (131)I-labelled IgG for targeting focal inflammation, which means MIF can be used as a better marker of inflammatory detection.

Show MeSH
Related in: MedlinePlus