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Embryonic stem cell-derived cardiomyocytes as a model to study fetal arrhythmia related to maternal disease.

Abdul Kadir SH, Ali NN, Mioulane M, Brito-Martins M, Abu-Hayyeh S, Foldes G, Moshkov AV, Williamson C, Harding SE, Gorelik J - J. Cell. Mol. Med. (2009)

Bottom Line: However, the mechanisms of the observed bile acid effects are not fully understood and their in vivo study in human beings is difficult.We show that early ESC-CM exhibit bile acid-induced disruption of rhythm, depression of contraction and desynchronization of cell coupling.This represents the first demonstration of the use of ESC-CM as a model system for human cardiac pathology, and opens the way for both investigation of mechanisms and a high throughput screen for drug discovery.

View Article: PubMed Central - PubMed

Affiliation: National Heart and Lung Institute, Imperial College London, London, UK.

ABSTRACT
Embryonic stem cell-derived cardiomyocytes (ESC-CM) have many of the phenotypic properties of authentic cardiomyocytes, and great interest has been shown in their possibilities for modelling human disease. Obstetric cholestasis affects 1 in 200 pregnant women in the United Kingdom. It is characterized by raised serum bile acids and complicated by premature delivery and unexplained fetal death at late gestation. It has been suggested that the fetal death is caused by the enhanced arrhythmogenic effect of bile acids in the fetal heart, and shown that neonatal susceptibility to bile acid-induced arrhythmia is lost in the adult rat cardiomyocyte. However, the mechanisms of the observed bile acid effects are not fully understood and their in vivo study in human beings is difficult. Here we use ESC-CM from both human and mouse ESCs to test our proposal that immature cardiomyocytes are more susceptible to the effect of raised bile acids than mature ones. We show that early ESC-CM exhibit bile acid-induced disruption of rhythm, depression of contraction and desynchronization of cell coupling. In both species the ESC-CM become resistant to these arrhythmias as the cells mature, and this develops in line with the respective gestational periods of mouse and human. This represents the first demonstration of the use of ESC-CM as a model system for human cardiac pathology, and opens the way for both investigation of mechanisms and a high throughput screen for drug discovery.

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(A) Example trace for contraction of human adult cardiomyocytes in 0.1, 1 and 3 mM TC. (B) Effect of TC on % of shortening of human adult cardiomyocytes: 3 mM concentration is not shown because of the disruption of rate at this concentration. (C) Effect of TC on times to peak contraction (TTP) and to 50% and 90% relaxation (R50 and R90) in human adult cardiomyocytes.
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fig09: (A) Example trace for contraction of human adult cardiomyocytes in 0.1, 1 and 3 mM TC. (B) Effect of TC on % of shortening of human adult cardiomyocytes: 3 mM concentration is not shown because of the disruption of rate at this concentration. (C) Effect of TC on times to peak contraction (TTP) and to 50% and 90% relaxation (R50 and R90) in human adult cardiomyocytes.

Mentions: The influence of TC was also studied in human adult ventricular cardiomyocytes. Rhythm was maintained in the paced cardiomyocytes, and there was no significant effect on contraction amplitude, time-to-peak contraction or time-to-50% or 90% relaxation following treatment with 0.1 mM to 1 mM TC (Fig. 9). However addition of 3 mM TC to the culture medium caused arrhythmia (Fig. 9A).


Embryonic stem cell-derived cardiomyocytes as a model to study fetal arrhythmia related to maternal disease.

Abdul Kadir SH, Ali NN, Mioulane M, Brito-Martins M, Abu-Hayyeh S, Foldes G, Moshkov AV, Williamson C, Harding SE, Gorelik J - J. Cell. Mol. Med. (2009)

(A) Example trace for contraction of human adult cardiomyocytes in 0.1, 1 and 3 mM TC. (B) Effect of TC on % of shortening of human adult cardiomyocytes: 3 mM concentration is not shown because of the disruption of rate at this concentration. (C) Effect of TC on times to peak contraction (TTP) and to 50% and 90% relaxation (R50 and R90) in human adult cardiomyocytes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4516522&req=5

fig09: (A) Example trace for contraction of human adult cardiomyocytes in 0.1, 1 and 3 mM TC. (B) Effect of TC on % of shortening of human adult cardiomyocytes: 3 mM concentration is not shown because of the disruption of rate at this concentration. (C) Effect of TC on times to peak contraction (TTP) and to 50% and 90% relaxation (R50 and R90) in human adult cardiomyocytes.
Mentions: The influence of TC was also studied in human adult ventricular cardiomyocytes. Rhythm was maintained in the paced cardiomyocytes, and there was no significant effect on contraction amplitude, time-to-peak contraction or time-to-50% or 90% relaxation following treatment with 0.1 mM to 1 mM TC (Fig. 9). However addition of 3 mM TC to the culture medium caused arrhythmia (Fig. 9A).

Bottom Line: However, the mechanisms of the observed bile acid effects are not fully understood and their in vivo study in human beings is difficult.We show that early ESC-CM exhibit bile acid-induced disruption of rhythm, depression of contraction and desynchronization of cell coupling.This represents the first demonstration of the use of ESC-CM as a model system for human cardiac pathology, and opens the way for both investigation of mechanisms and a high throughput screen for drug discovery.

View Article: PubMed Central - PubMed

Affiliation: National Heart and Lung Institute, Imperial College London, London, UK.

ABSTRACT
Embryonic stem cell-derived cardiomyocytes (ESC-CM) have many of the phenotypic properties of authentic cardiomyocytes, and great interest has been shown in their possibilities for modelling human disease. Obstetric cholestasis affects 1 in 200 pregnant women in the United Kingdom. It is characterized by raised serum bile acids and complicated by premature delivery and unexplained fetal death at late gestation. It has been suggested that the fetal death is caused by the enhanced arrhythmogenic effect of bile acids in the fetal heart, and shown that neonatal susceptibility to bile acid-induced arrhythmia is lost in the adult rat cardiomyocyte. However, the mechanisms of the observed bile acid effects are not fully understood and their in vivo study in human beings is difficult. Here we use ESC-CM from both human and mouse ESCs to test our proposal that immature cardiomyocytes are more susceptible to the effect of raised bile acids than mature ones. We show that early ESC-CM exhibit bile acid-induced disruption of rhythm, depression of contraction and desynchronization of cell coupling. In both species the ESC-CM become resistant to these arrhythmias as the cells mature, and this develops in line with the respective gestational periods of mouse and human. This represents the first demonstration of the use of ESC-CM as a model system for human cardiac pathology, and opens the way for both investigation of mechanisms and a high throughput screen for drug discovery.

Show MeSH
Related in: MedlinePlus