Limits...
Preparation, Characterization, and Pharmacological Activity of Cymbopogon winterianus Jowitt ex Bor (Poaceae) Leaf Essential Oil of β-Cyclodextrin Inclusion Complexes.

Santos PL, Araújo AA, Quintans JS, Oliveira MG, Brito RG, Serafini MR, Menezes PP, Santos MR, Alves PB, de Lucca Júnior W, Blank AF, La Rocca V, Almeida RN, Quintans-Júnior LJ - Evid Based Complement Alternat Med (2015)

Bottom Line: The orofacial nociceptive behavior was significantly (p < 0.05) reduced.LEO-CD did not cause changes in motor coordination in the rota-rod test.Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.

ABSTRACT
This study aimed to evaluate the orofacial antinociceptive effect of the Cymbopogon winterianus essential oil (LEO) complexed in β-cyclodextrin (LEO-CD) and to assess the possible involvement of the central nervous system (CNS). The LEO was extracted, chromatographed, and complexed in β-cyclodextrin. The complex was characterized by differential scanning calorimetry (DSC) and thermogravimetry derivative (TG/DTG). Male Swiss mice (2-3 months) were treated with LEO-CD (50-200 mg/kg, p.o.), vehicle (distilled water, p.o.), or standard drug (i.p.) and subjected to the orofacial nociception formalin-, capsaicin-, and glutamate-induced. After the formalin test, the animals were perfused and the brains subjected to immunofluorescence for Fos. The rota-rod test (7 rpm/min) was carried out. Geraniol (37.57%) was the main compound of LEO. DSC and TG/DTG proved the complexation. The orofacial nociceptive behavior was significantly (p < 0.05) reduced. The number of Fos-positive cells was significantly changed in the dorsal raphe nucleus (p < 0.01), locus coeruleus (p < 0.001), trigeminal nucleus (p < 0.05), and trigeminal thalamic tract (p < 0.05). LEO-CD did not cause changes in motor coordination in the rota-rod test. Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.

No MeSH data available.


Related in: MedlinePlus

SEM micrographs of cross sections of (a) β-CD, (b) physical mixture (PM), and (c) slurry complex (SC). 1000x and 2000x.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4515525&req=5

fig2: SEM micrographs of cross sections of (a) β-CD, (b) physical mixture (PM), and (c) slurry complex (SC). 1000x and 2000x.

Mentions: SEM micrographs show the different magnifications of the β-CD, physical mixture (PM), and slurry complex (SC). By using the SEM, in Figure 2(a), one can see the different sizes of rectangular-shaped and small particles that adhered to the surfaces of the crystals. Figure 2(b) shows an aspect similar to Figure 2(a), which demonstrates the low capacity of molecular inclusion. However, Figure 2(c) allows observing the amorphous structure. The original morphology of the raw materials disappeared and this property is attributed to the formation of inclusion complexes.


Preparation, Characterization, and Pharmacological Activity of Cymbopogon winterianus Jowitt ex Bor (Poaceae) Leaf Essential Oil of β-Cyclodextrin Inclusion Complexes.

Santos PL, Araújo AA, Quintans JS, Oliveira MG, Brito RG, Serafini MR, Menezes PP, Santos MR, Alves PB, de Lucca Júnior W, Blank AF, La Rocca V, Almeida RN, Quintans-Júnior LJ - Evid Based Complement Alternat Med (2015)

SEM micrographs of cross sections of (a) β-CD, (b) physical mixture (PM), and (c) slurry complex (SC). 1000x and 2000x.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4515525&req=5

fig2: SEM micrographs of cross sections of (a) β-CD, (b) physical mixture (PM), and (c) slurry complex (SC). 1000x and 2000x.
Mentions: SEM micrographs show the different magnifications of the β-CD, physical mixture (PM), and slurry complex (SC). By using the SEM, in Figure 2(a), one can see the different sizes of rectangular-shaped and small particles that adhered to the surfaces of the crystals. Figure 2(b) shows an aspect similar to Figure 2(a), which demonstrates the low capacity of molecular inclusion. However, Figure 2(c) allows observing the amorphous structure. The original morphology of the raw materials disappeared and this property is attributed to the formation of inclusion complexes.

Bottom Line: The orofacial nociceptive behavior was significantly (p < 0.05) reduced.LEO-CD did not cause changes in motor coordination in the rota-rod test.Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.

ABSTRACT
This study aimed to evaluate the orofacial antinociceptive effect of the Cymbopogon winterianus essential oil (LEO) complexed in β-cyclodextrin (LEO-CD) and to assess the possible involvement of the central nervous system (CNS). The LEO was extracted, chromatographed, and complexed in β-cyclodextrin. The complex was characterized by differential scanning calorimetry (DSC) and thermogravimetry derivative (TG/DTG). Male Swiss mice (2-3 months) were treated with LEO-CD (50-200 mg/kg, p.o.), vehicle (distilled water, p.o.), or standard drug (i.p.) and subjected to the orofacial nociception formalin-, capsaicin-, and glutamate-induced. After the formalin test, the animals were perfused and the brains subjected to immunofluorescence for Fos. The rota-rod test (7 rpm/min) was carried out. Geraniol (37.57%) was the main compound of LEO. DSC and TG/DTG proved the complexation. The orofacial nociceptive behavior was significantly (p < 0.05) reduced. The number of Fos-positive cells was significantly changed in the dorsal raphe nucleus (p < 0.01), locus coeruleus (p < 0.001), trigeminal nucleus (p < 0.05), and trigeminal thalamic tract (p < 0.05). LEO-CD did not cause changes in motor coordination in the rota-rod test. Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.

No MeSH data available.


Related in: MedlinePlus