Limits...
Costus afer Possesses Carbohydrate Hydrolyzing Enzymes Inhibitory Activity and Antioxidant Capacity In Vitro.

Tchamgoue AD, Tchokouaha LR, Tarkang PA, Kuiate JR, Agbor GA - Evid Based Complement Alternat Med (2015)

Bottom Line: Kinetic analysis revealed two modes of enzyme inhibition (competitive and mixed).All extracts showed antioxidant capacity, with hexane extracts exhibiting the best activity.The presence of bioactive compounds such as flavonoids, alkaloids, phenols, and tannins may account for the antioxidant capacity and carbohydrate hydrolyzing enzyme inhibitory activity of C. afer.

View Article: PubMed Central - PubMed

Affiliation: Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, BP 6163, Yaoundé, Cameroon ; Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Diabetes mellitus is a metabolic disorder of glucose metabolism which correlates with postprandial hyperglycemia and oxidative stress. Control of blood glucose level is imperative in the management of diabetes. The present study tested the hypothesis that Costus afer, an antihyperglycemic medicinal plant, possesses inhibitory activity against carbohydrate hydrolyzing enzymes. Hexane, ethyl acetate, methanol, and water extracts were prepared from the leaf, stem, and rhizome of C. afer and subjected to phytochemical screening, assayed for α-amylase and α-glucosidase inhibitory activities and antioxidant capacity (determined by total phenolic and total flavonoids contents, ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity). All extracts inhibited α-amylase and α-glucosidase activities. Ethyl acetate rhizome and methanol leaf extracts exhibited the best inhibitory activity against α-amylase and α-glucosidase (IC50: 0.10 and 5.99 mg/mL), respectively. Kinetic analysis revealed two modes of enzyme inhibition (competitive and mixed). All extracts showed antioxidant capacity, with hexane extracts exhibiting the best activity. DPPH assay revealed that methanol leaf, rhizome, and ethyl acetate stem extracts (IC50 < 5 mg/mL) were the best antioxidants. The presence of bioactive compounds such as flavonoids, alkaloids, phenols, and tannins may account for the antioxidant capacity and carbohydrate hydrolyzing enzyme inhibitory activity of C. afer.

No MeSH data available.


Related in: MedlinePlus

Lineweaver-Burk plots of activity of α-glucosidase from Bacillus stearothermophilus in the absence (control) or presence of the methanol leaf, stem, and rhizome extracts.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4515504&req=5

fig5: Lineweaver-Burk plots of activity of α-glucosidase from Bacillus stearothermophilus in the absence (control) or presence of the methanol leaf, stem, and rhizome extracts.

Mentions: Among all the extracts, only methanol leaf extract inhibited both α-amylase and α-glucosidase. Ethyl acetate leaf extract inhibited α-amylase through a mixed mechanism with Vm and Km different from those of the control (Figure 4, Table 4). Methanol leaf, water stem, and rhizome extracts exerted a competitive inhibition vis-à-vis this enzyme showing the same Vm and different Km compared to the control (Figure 4). On α-glucosidase, it was observed that the methanol leaf extract showed mixed inhibitory mode while the methanol stem and rhizome extracts had a competitive inhibitory mode (Figure 5, Table 4).


Costus afer Possesses Carbohydrate Hydrolyzing Enzymes Inhibitory Activity and Antioxidant Capacity In Vitro.

Tchamgoue AD, Tchokouaha LR, Tarkang PA, Kuiate JR, Agbor GA - Evid Based Complement Alternat Med (2015)

Lineweaver-Burk plots of activity of α-glucosidase from Bacillus stearothermophilus in the absence (control) or presence of the methanol leaf, stem, and rhizome extracts.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4515504&req=5

fig5: Lineweaver-Burk plots of activity of α-glucosidase from Bacillus stearothermophilus in the absence (control) or presence of the methanol leaf, stem, and rhizome extracts.
Mentions: Among all the extracts, only methanol leaf extract inhibited both α-amylase and α-glucosidase. Ethyl acetate leaf extract inhibited α-amylase through a mixed mechanism with Vm and Km different from those of the control (Figure 4, Table 4). Methanol leaf, water stem, and rhizome extracts exerted a competitive inhibition vis-à-vis this enzyme showing the same Vm and different Km compared to the control (Figure 4). On α-glucosidase, it was observed that the methanol leaf extract showed mixed inhibitory mode while the methanol stem and rhizome extracts had a competitive inhibitory mode (Figure 5, Table 4).

Bottom Line: Kinetic analysis revealed two modes of enzyme inhibition (competitive and mixed).All extracts showed antioxidant capacity, with hexane extracts exhibiting the best activity.The presence of bioactive compounds such as flavonoids, alkaloids, phenols, and tannins may account for the antioxidant capacity and carbohydrate hydrolyzing enzyme inhibitory activity of C. afer.

View Article: PubMed Central - PubMed

Affiliation: Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, BP 6163, Yaoundé, Cameroon ; Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Diabetes mellitus is a metabolic disorder of glucose metabolism which correlates with postprandial hyperglycemia and oxidative stress. Control of blood glucose level is imperative in the management of diabetes. The present study tested the hypothesis that Costus afer, an antihyperglycemic medicinal plant, possesses inhibitory activity against carbohydrate hydrolyzing enzymes. Hexane, ethyl acetate, methanol, and water extracts were prepared from the leaf, stem, and rhizome of C. afer and subjected to phytochemical screening, assayed for α-amylase and α-glucosidase inhibitory activities and antioxidant capacity (determined by total phenolic and total flavonoids contents, ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity). All extracts inhibited α-amylase and α-glucosidase activities. Ethyl acetate rhizome and methanol leaf extracts exhibited the best inhibitory activity against α-amylase and α-glucosidase (IC50: 0.10 and 5.99 mg/mL), respectively. Kinetic analysis revealed two modes of enzyme inhibition (competitive and mixed). All extracts showed antioxidant capacity, with hexane extracts exhibiting the best activity. DPPH assay revealed that methanol leaf, rhizome, and ethyl acetate stem extracts (IC50 < 5 mg/mL) were the best antioxidants. The presence of bioactive compounds such as flavonoids, alkaloids, phenols, and tannins may account for the antioxidant capacity and carbohydrate hydrolyzing enzyme inhibitory activity of C. afer.

No MeSH data available.


Related in: MedlinePlus