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A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers.

Nilsen T, Sunde K, Larsson A - J Inflamm (Lond) (2015)

Bottom Line: The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens.The performance was tested and it was observed that the assay was linear in the range 0.3-24.7 mg/L, the limit of quantitation was observed to be lower than 0.3 mg/L, no antigen excess was observed up to 54 mg/L, all CVs were lower than 1.8 % in the precision study, the calibration curve stability was longer than 6 weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin.In addition it is commutable with Bühlmann MRP8/14 ELISA.

View Article: PubMed Central - PubMed

Affiliation: Gentian Technology AS, Moss, Norway ; Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden.

ABSTRACT
Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3-24.7¬†mg/L, the limit of quantitation was observed to be lower than 0.3¬†mg/L, no antigen excess was observed up to 54¬†mg/L, all CVs were lower than 1.8¬†% in the precision study, the calibration curve stability was longer than 6¬†weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with B√ľhlmann MRP8/14 ELISA.

No MeSH data available.


Related in: MedlinePlus

Samples 1 and 4 measured within the range of baseline ¬Ī10¬†% for all 6¬†weeks. From the observed results, the turbidimetric serum calprotectin immunoassay can claim 6¬†week of calibration curve stability for samples higher than 6¬†mg/L
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Related In: Results  -  Collection

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Fig4: Samples 1 and 4 measured within the range of baseline ¬Ī10¬†% for all 6¬†weeks. From the observed results, the turbidimetric serum calprotectin immunoassay can claim 6¬†week of calibration curve stability for samples higher than 6¬†mg/L

Mentions: The observation of the measured samples over time, shown in Figs. 3 and 4, suggests a calibration curve stability of at least 6 weeks. The total CV for all samples was less than 2 %. The concentration of samples 1, 2, 3, 4 and 5 were 1.17, 6.82, 22.65, 1.22 and 8.78 mg/L respectively at baseline.Fig. 3


A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers.

Nilsen T, Sunde K, Larsson A - J Inflamm (Lond) (2015)

Samples 1 and 4 measured within the range of baseline ¬Ī10¬†% for all 6¬†weeks. From the observed results, the turbidimetric serum calprotectin immunoassay can claim 6¬†week of calibration curve stability for samples higher than 6¬†mg/L
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4514941&req=5

Fig4: Samples 1 and 4 measured within the range of baseline ¬Ī10¬†% for all 6¬†weeks. From the observed results, the turbidimetric serum calprotectin immunoassay can claim 6¬†week of calibration curve stability for samples higher than 6¬†mg/L
Mentions: The observation of the measured samples over time, shown in Figs. 3 and 4, suggests a calibration curve stability of at least 6 weeks. The total CV for all samples was less than 2 %. The concentration of samples 1, 2, 3, 4 and 5 were 1.17, 6.82, 22.65, 1.22 and 8.78 mg/L respectively at baseline.Fig. 3

Bottom Line: The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens.The performance was tested and it was observed that the assay was linear in the range 0.3-24.7 mg/L, the limit of quantitation was observed to be lower than 0.3 mg/L, no antigen excess was observed up to 54 mg/L, all CVs were lower than 1.8 % in the precision study, the calibration curve stability was longer than 6 weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin.In addition it is commutable with Bühlmann MRP8/14 ELISA.

View Article: PubMed Central - PubMed

Affiliation: Gentian Technology AS, Moss, Norway ; Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden.

ABSTRACT
Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3-24.7¬†mg/L, the limit of quantitation was observed to be lower than 0.3¬†mg/L, no antigen excess was observed up to 54¬†mg/L, all CVs were lower than 1.8¬†% in the precision study, the calibration curve stability was longer than 6¬†weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with B√ľhlmann MRP8/14 ELISA.

No MeSH data available.


Related in: MedlinePlus