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Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'.

Wang N, Zheng Y, Duan N, Zhang Z, Ji X, Jiang S, Sun S, Yang L, Bai Y, Fei Z, Chen X - PLoS ONE (2015)

Bottom Line: Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses.Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR.After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.

ABSTRACT
Transcriptome profiles of the red- and white-fleshed apples in an F1 segregating population of Malus sieversii f.Niedzwetzkyana and M.domestica 'Fuji' were generated using the next-generation high-throughput RNA sequencing (RNA-Seq) technology and compared. A total of 114 differentially expressed genes (DEGs) were obtained, of which 88 were up-regulated and 26 were down-regulated in red-fleshed apples. The 88 up-regulated genes were enriched with those related to flavonoid biosynthetic process and stress responses. Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses. Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR. After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple. Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.

No MeSH data available.


Related in: MedlinePlus

Analysis of differentially expressed genes (DEGs).(A) Numbers of up- and down-regulated genes between red- and white-fleshed fruits at the ripe stage. (B) Distribution of fold changes (FC) for up- and down-regulated genes at the ripe stage.
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pone.0133468.g002: Analysis of differentially expressed genes (DEGs).(A) Numbers of up- and down-regulated genes between red- and white-fleshed fruits at the ripe stage. (B) Distribution of fold changes (FC) for up- and down-regulated genes at the ripe stage.

Mentions: A total of 114 differentially expressed genes (DEGs)(S5 Table), including 88 up-regulated and 26 down-regulated, were identified in red-fleshed apples compared with white-fleshed apples (Fig 2A). The log2ratio of the gene expression ranged from -7.3 (down-regulated) to 8.4 (up-regulated).


Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'.

Wang N, Zheng Y, Duan N, Zhang Z, Ji X, Jiang S, Sun S, Yang L, Bai Y, Fei Z, Chen X - PLoS ONE (2015)

Analysis of differentially expressed genes (DEGs).(A) Numbers of up- and down-regulated genes between red- and white-fleshed fruits at the ripe stage. (B) Distribution of fold changes (FC) for up- and down-regulated genes at the ripe stage.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4514764&req=5

pone.0133468.g002: Analysis of differentially expressed genes (DEGs).(A) Numbers of up- and down-regulated genes between red- and white-fleshed fruits at the ripe stage. (B) Distribution of fold changes (FC) for up- and down-regulated genes at the ripe stage.
Mentions: A total of 114 differentially expressed genes (DEGs)(S5 Table), including 88 up-regulated and 26 down-regulated, were identified in red-fleshed apples compared with white-fleshed apples (Fig 2A). The log2ratio of the gene expression ranged from -7.3 (down-regulated) to 8.4 (up-regulated).

Bottom Line: Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses.Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR.After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.

ABSTRACT
Transcriptome profiles of the red- and white-fleshed apples in an F1 segregating population of Malus sieversii f.Niedzwetzkyana and M.domestica 'Fuji' were generated using the next-generation high-throughput RNA sequencing (RNA-Seq) technology and compared. A total of 114 differentially expressed genes (DEGs) were obtained, of which 88 were up-regulated and 26 were down-regulated in red-fleshed apples. The 88 up-regulated genes were enriched with those related to flavonoid biosynthetic process and stress responses. Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses. Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR. After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple. Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.

No MeSH data available.


Related in: MedlinePlus