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Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'.

Wang N, Zheng Y, Duan N, Zhang Z, Ji X, Jiang S, Sun S, Yang L, Bai Y, Fei Z, Chen X - PLoS ONE (2015)

Bottom Line: Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses.Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR.Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.

ABSTRACT
Transcriptome profiles of the red- and white-fleshed apples in an F1 segregating population of Malus sieversii f.Niedzwetzkyana and M.domestica 'Fuji' were generated using the next-generation high-throughput RNA sequencing (RNA-Seq) technology and compared. A total of 114 differentially expressed genes (DEGs) were obtained, of which 88 were up-regulated and 26 were down-regulated in red-fleshed apples. The 88 up-regulated genes were enriched with those related to flavonoid biosynthetic process and stress responses. Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses. Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR. After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple. Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.

No MeSH data available.


Related in: MedlinePlus

Red- and white-fleshed apples at the ripe stage used for RNA-Seq.(A)Red-fleshed apples in the F1 population. Scale bar = 1cm. (B) White-fleshed apples in the F1 population. Scale bar = 1cm. (C) Flavonoid content in red- and white-fleshed apples. (D) Anthocyanin content in red- and white-fleshed apples.
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pone.0133468.g001: Red- and white-fleshed apples at the ripe stage used for RNA-Seq.(A)Red-fleshed apples in the F1 population. Scale bar = 1cm. (B) White-fleshed apples in the F1 population. Scale bar = 1cm. (C) Flavonoid content in red- and white-fleshed apples. (D) Anthocyanin content in red- and white-fleshed apples.

Mentions: An F1 hybrid population derived from a cross between M.sieversii f. niedzwetzkyana and M.domestica ‘Fuji’ grown in the Shandong Agricultural University Tai’an Hengling Fruit tree breeding base(36°26′ N, 117°29′ E)was used in this study. Red- and white-fleshed fruits at the ripe developmental stage (Fig 1A and 1B) were harvested in biological triplicates, each from 20 F1 seedlings, then frozen in liquid nitrogen and stored at -80°C until use.


Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'.

Wang N, Zheng Y, Duan N, Zhang Z, Ji X, Jiang S, Sun S, Yang L, Bai Y, Fei Z, Chen X - PLoS ONE (2015)

Red- and white-fleshed apples at the ripe stage used for RNA-Seq.(A)Red-fleshed apples in the F1 population. Scale bar = 1cm. (B) White-fleshed apples in the F1 population. Scale bar = 1cm. (C) Flavonoid content in red- and white-fleshed apples. (D) Anthocyanin content in red- and white-fleshed apples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4514764&req=5

pone.0133468.g001: Red- and white-fleshed apples at the ripe stage used for RNA-Seq.(A)Red-fleshed apples in the F1 population. Scale bar = 1cm. (B) White-fleshed apples in the F1 population. Scale bar = 1cm. (C) Flavonoid content in red- and white-fleshed apples. (D) Anthocyanin content in red- and white-fleshed apples.
Mentions: An F1 hybrid population derived from a cross between M.sieversii f. niedzwetzkyana and M.domestica ‘Fuji’ grown in the Shandong Agricultural University Tai’an Hengling Fruit tree breeding base(36°26′ N, 117°29′ E)was used in this study. Red- and white-fleshed fruits at the ripe developmental stage (Fig 1A and 1B) were harvested in biological triplicates, each from 20 F1 seedlings, then frozen in liquid nitrogen and stored at -80°C until use.

Bottom Line: Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses.Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR.Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.

ABSTRACT
Transcriptome profiles of the red- and white-fleshed apples in an F1 segregating population of Malus sieversii f.Niedzwetzkyana and M.domestica 'Fuji' were generated using the next-generation high-throughput RNA sequencing (RNA-Seq) technology and compared. A total of 114 differentially expressed genes (DEGs) were obtained, of which 88 were up-regulated and 26 were down-regulated in red-fleshed apples. The 88 up-regulated genes were enriched with those related to flavonoid biosynthetic process and stress responses. Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses. Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR. After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple. Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.

No MeSH data available.


Related in: MedlinePlus