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LC-MS/MS Method for Serum Creatinine: Comparison with Enzymatic Method and Jaffe Method.

Ou M, Song Y, Li S, Liu G, Jia J, Zhang M, Zhang H, Yu C - PLoS ONE (2015)

Bottom Line: Accurate quantification of creatinine (Cre) is important to estimate glomerular filtration rate (GFR).LC-MS/MS analysis was performed on API 4000 triple quadrupole mass spectrometer coupled with an Agilent 1200 liquid chromatography system.The reference intervals of serum Cre measured by LC-MS/MS assay were 41-79 μmol/L for adult women, and 46-101 μmol/L for adult men.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Clinical Research Center, Chinese Academy of Sciences, Shanghai, China; Central Laboratory, Shanghai Xuhui Central Hospital, Shanghai, China.

ABSTRACT
Accurate quantification of creatinine (Cre) is important to estimate glomerular filtration rate (GFR). Differences among various methods of Cre quantification were previously noted. This study aims to develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for serum Cre and compare this method with clinical routine methods. LC-MS/MS analysis was performed on API 4000 triple quadrupole mass spectrometer coupled with an Agilent 1200 liquid chromatography system. After adding isotope-labeled Cre-d3 as internal standard, serum samples were prepared via a one-step protein precipitation with methanol. The LC-MS/MS method was compared with frequently used enzymatic method and Jaffe method. This developed method, with a total run time of 3 min, had a lower limit of quantification of 4.4 μmol/L, a total imprecision of 1.15%-3.84%, and an average bias of 1.06%. No significant matrix effect, carryover, and interference were observed for the LC-MS/MS method. The reference intervals of serum Cre measured by LC-MS/MS assay were 41-79 μmol/L for adult women, and 46-101 μmol/L for adult men. Using LC-MS/MS as a reference, the enzymatic method showed an average bias of -2.1% and the Jaffe method showed a substantial average bias of 11.7%. Compared with the LC-MS/MS method, significant negative bias was observed for the enzymatic and Jaffe methods in hemolytic and lipimic samples. We developed a simple, specific, and accurate LC-MS/MS method to analyze serum Cre. Discordance existed among different methods.

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Method comparison of the serum Cre with (1) Deming regression and (2) Bland-Altman analysis of (A) LC-MS/MS versus enzymatic method, (B) LC-MS/MS versus Jaffe method, and (C) enzymatic method versus Jaffe method.
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pone.0133912.g003: Method comparison of the serum Cre with (1) Deming regression and (2) Bland-Altman analysis of (A) LC-MS/MS versus enzymatic method, (B) LC-MS/MS versus Jaffe method, and (C) enzymatic method versus Jaffe method.

Mentions: Patient serum samples collected from 100 men and 262 women were split into three aliquots, and were stored at -20°C until analysis. Serum Cre was measured in a single run using the LC-MS/MS, enzymatic and Jaffe methods. Results were compared by Deming regression and Bland-Altman plot (Fig 3). Enzymatic and Jaffe methods showed a mean proportional difference of -2.1% and 11.7%, respectively, compared with LC-MS/MS. In the Bland-Altman analysis, the 95% limit of agreement ranged from -13.2% to 9.0% for the enzymatic method and from -6.5% to 29.9% for the Jaffe method between the proposed LC-MS/MS assay. Between-method parameters of Bland-Altman mean differences, Deming regression analysis and Pearson's correlation coefficients are presented in S1 Table.


LC-MS/MS Method for Serum Creatinine: Comparison with Enzymatic Method and Jaffe Method.

Ou M, Song Y, Li S, Liu G, Jia J, Zhang M, Zhang H, Yu C - PLoS ONE (2015)

Method comparison of the serum Cre with (1) Deming regression and (2) Bland-Altman analysis of (A) LC-MS/MS versus enzymatic method, (B) LC-MS/MS versus Jaffe method, and (C) enzymatic method versus Jaffe method.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4514740&req=5

pone.0133912.g003: Method comparison of the serum Cre with (1) Deming regression and (2) Bland-Altman analysis of (A) LC-MS/MS versus enzymatic method, (B) LC-MS/MS versus Jaffe method, and (C) enzymatic method versus Jaffe method.
Mentions: Patient serum samples collected from 100 men and 262 women were split into three aliquots, and were stored at -20°C until analysis. Serum Cre was measured in a single run using the LC-MS/MS, enzymatic and Jaffe methods. Results were compared by Deming regression and Bland-Altman plot (Fig 3). Enzymatic and Jaffe methods showed a mean proportional difference of -2.1% and 11.7%, respectively, compared with LC-MS/MS. In the Bland-Altman analysis, the 95% limit of agreement ranged from -13.2% to 9.0% for the enzymatic method and from -6.5% to 29.9% for the Jaffe method between the proposed LC-MS/MS assay. Between-method parameters of Bland-Altman mean differences, Deming regression analysis and Pearson's correlation coefficients are presented in S1 Table.

Bottom Line: Accurate quantification of creatinine (Cre) is important to estimate glomerular filtration rate (GFR).LC-MS/MS analysis was performed on API 4000 triple quadrupole mass spectrometer coupled with an Agilent 1200 liquid chromatography system.The reference intervals of serum Cre measured by LC-MS/MS assay were 41-79 μmol/L for adult women, and 46-101 μmol/L for adult men.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Clinical Research Center, Chinese Academy of Sciences, Shanghai, China; Central Laboratory, Shanghai Xuhui Central Hospital, Shanghai, China.

ABSTRACT
Accurate quantification of creatinine (Cre) is important to estimate glomerular filtration rate (GFR). Differences among various methods of Cre quantification were previously noted. This study aims to develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for serum Cre and compare this method with clinical routine methods. LC-MS/MS analysis was performed on API 4000 triple quadrupole mass spectrometer coupled with an Agilent 1200 liquid chromatography system. After adding isotope-labeled Cre-d3 as internal standard, serum samples were prepared via a one-step protein precipitation with methanol. The LC-MS/MS method was compared with frequently used enzymatic method and Jaffe method. This developed method, with a total run time of 3 min, had a lower limit of quantification of 4.4 μmol/L, a total imprecision of 1.15%-3.84%, and an average bias of 1.06%. No significant matrix effect, carryover, and interference were observed for the LC-MS/MS method. The reference intervals of serum Cre measured by LC-MS/MS assay were 41-79 μmol/L for adult women, and 46-101 μmol/L for adult men. Using LC-MS/MS as a reference, the enzymatic method showed an average bias of -2.1% and the Jaffe method showed a substantial average bias of 11.7%. Compared with the LC-MS/MS method, significant negative bias was observed for the enzymatic and Jaffe methods in hemolytic and lipimic samples. We developed a simple, specific, and accurate LC-MS/MS method to analyze serum Cre. Discordance existed among different methods.

No MeSH data available.


Related in: MedlinePlus