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The Corn Smut ('Huitlacoche') as a New Platform for Oral Vaccines.

Juárez-Montiel M, Romero-Maldonado A, Monreal-Escalante E, Becerra-Flora A, Korban SS, Rosales-Mendoza S, Jiménez-Bremont JF - PLoS ONE (2015)

Bottom Line: The development of new alternative platforms for subunit vaccine production is a priority in the biomedical field.These findings demonstrate the feasibility of using edible corn smut as a safe, effective, and low-cost platform for production and delivery of a subunit oral vaccine.The implications of this platform in the area of molecular pharming are discussed.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Estudios Moleculares de Respuesta a Estrés en Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC, San Luis Potosí, San Luis Potosí, México.

ABSTRACT
The development of new alternative platforms for subunit vaccine production is a priority in the biomedical field. In this study, Ustilago maydis, the causal agent of common corn smut or 'huitlacoche'has been genetically engineered to assess expression and immunogenicity of the B subunit of the cholera toxin (CTB), a relevant immunomodulatory agent in vaccinology. An oligomeric CTB recombinant protein was expressed in corn smut galls at levels of up to 1.3 mg g-1 dry weight (0.8% of the total soluble protein). Mice orally immunized with 'huitlacoche'-derived CTB showed significant humoral responses that were well-correlated with protection against challenge with the cholera toxin (CT). These findings demonstrate the feasibility of using edible corn smut as a safe, effective, and low-cost platform for production and delivery of a subunit oral vaccine. The implications of this platform in the area of molecular pharming are discussed.

No MeSH data available.


Related in: MedlinePlus

Antibody levels of BALB/c mice immunized with corn smut galls expressing CTB protein.Detection of: (a) anti-CTB IgA antibodies in intestinal washes, and (b) anti-CTB IgG antibodies in sera. Mice immunized with different treatments (biomass from ‘huitlacoche’: FB 1 x FB2-CTB 3 and FB1 x FB2 as control) were bled at day 21, and serum was diluted 1:40. Statistical differences versus the WT group are indicated by an asterisk (P < 0.05).
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pone.0133535.g005: Antibody levels of BALB/c mice immunized with corn smut galls expressing CTB protein.Detection of: (a) anti-CTB IgA antibodies in intestinal washes, and (b) anti-CTB IgG antibodies in sera. Mice immunized with different treatments (biomass from ‘huitlacoche’: FB 1 x FB2-CTB 3 and FB1 x FB2 as control) were bled at day 21, and serum was diluted 1:40. Statistical differences versus the WT group are indicated by an asterisk (P < 0.05).

Mentions: Following a three-week immunization scheme, mice orally immunized with transgenic FB1 x FB2-CTB 3 ‘huitlacoche’showed significantly higher intestinal IgA and serum IgG levels than those immunized with WT ‘huitlacoche’ (Fig 5a and 5b). The anti-CTB IgG titer for the FB1 x FB2-CTB 3 ‘huitlacoche’-immunized group was 40. When mice were challenged with the cholera toxin (CT), fluid accumulation (FA) levels were significantly lower (mean of 51.8) for mice immunized with transgenic ‘huitlacoche’ than those immunized with wild-type tissues (mean of 93.1). In contrast, FA levels of unchallenged mice showed a mean of 52.2 (Fig 6). The observed protective effects induced by the CTB protein produced in transgenic ‘huitlacoche’suggested elicitation of a humoral response in intestinal mucosal tissues in mice.


The Corn Smut ('Huitlacoche') as a New Platform for Oral Vaccines.

Juárez-Montiel M, Romero-Maldonado A, Monreal-Escalante E, Becerra-Flora A, Korban SS, Rosales-Mendoza S, Jiménez-Bremont JF - PLoS ONE (2015)

Antibody levels of BALB/c mice immunized with corn smut galls expressing CTB protein.Detection of: (a) anti-CTB IgA antibodies in intestinal washes, and (b) anti-CTB IgG antibodies in sera. Mice immunized with different treatments (biomass from ‘huitlacoche’: FB 1 x FB2-CTB 3 and FB1 x FB2 as control) were bled at day 21, and serum was diluted 1:40. Statistical differences versus the WT group are indicated by an asterisk (P < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4514630&req=5

pone.0133535.g005: Antibody levels of BALB/c mice immunized with corn smut galls expressing CTB protein.Detection of: (a) anti-CTB IgA antibodies in intestinal washes, and (b) anti-CTB IgG antibodies in sera. Mice immunized with different treatments (biomass from ‘huitlacoche’: FB 1 x FB2-CTB 3 and FB1 x FB2 as control) were bled at day 21, and serum was diluted 1:40. Statistical differences versus the WT group are indicated by an asterisk (P < 0.05).
Mentions: Following a three-week immunization scheme, mice orally immunized with transgenic FB1 x FB2-CTB 3 ‘huitlacoche’showed significantly higher intestinal IgA and serum IgG levels than those immunized with WT ‘huitlacoche’ (Fig 5a and 5b). The anti-CTB IgG titer for the FB1 x FB2-CTB 3 ‘huitlacoche’-immunized group was 40. When mice were challenged with the cholera toxin (CT), fluid accumulation (FA) levels were significantly lower (mean of 51.8) for mice immunized with transgenic ‘huitlacoche’ than those immunized with wild-type tissues (mean of 93.1). In contrast, FA levels of unchallenged mice showed a mean of 52.2 (Fig 6). The observed protective effects induced by the CTB protein produced in transgenic ‘huitlacoche’suggested elicitation of a humoral response in intestinal mucosal tissues in mice.

Bottom Line: The development of new alternative platforms for subunit vaccine production is a priority in the biomedical field.These findings demonstrate the feasibility of using edible corn smut as a safe, effective, and low-cost platform for production and delivery of a subunit oral vaccine.The implications of this platform in the area of molecular pharming are discussed.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Estudios Moleculares de Respuesta a Estrés en Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC, San Luis Potosí, San Luis Potosí, México.

ABSTRACT
The development of new alternative platforms for subunit vaccine production is a priority in the biomedical field. In this study, Ustilago maydis, the causal agent of common corn smut or 'huitlacoche'has been genetically engineered to assess expression and immunogenicity of the B subunit of the cholera toxin (CTB), a relevant immunomodulatory agent in vaccinology. An oligomeric CTB recombinant protein was expressed in corn smut galls at levels of up to 1.3 mg g-1 dry weight (0.8% of the total soluble protein). Mice orally immunized with 'huitlacoche'-derived CTB showed significant humoral responses that were well-correlated with protection against challenge with the cholera toxin (CT). These findings demonstrate the feasibility of using edible corn smut as a safe, effective, and low-cost platform for production and delivery of a subunit oral vaccine. The implications of this platform in the area of molecular pharming are discussed.

No MeSH data available.


Related in: MedlinePlus