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Expression Level of Genes Coding for Cell Adhesion Molecules of Cadherin Group in Colorectal Cancer Patients.

Lorenc Z, Opiłka MN, Kruszniewska-Rajs C, Rajs A, Waniczek D, Starzewska M, Lorenc J, Mazurek U - Med. Sci. Monit. (2015)

Bottom Line: The results were validated using qRT-PCR technique.Agglomerative hierarchical clustering of normalized mRNA levels has shown 4 groups with statistically different gene expression.Consolidated results of the fluorescency of all of the differential genes, designated two coding E-cadherin (CDH1) with the lower expression, and P-cadherin (CDH3) with higher expression in CRC tissue.

View Article: PubMed Central - PubMed

Affiliation: Chair and Clinical Department of General, Colorectal and Trauma Surgery, Medical University of Silesia, Katowice, Poland.

ABSTRACT

Background: Colorectal Cancer (CRC) is one of the most frequently diagnosed neoplasms and also one of the main death causes. Cell adhesion molecules are taking part in specific junctions, contributing to tissue integrality. Lower expression of the cadherins may be correlated with poorer differentiation of the CRC, and its more aggressive phenotype. The aim of the study is to designate the cadherin genes potentially useful for the diagnostics, prognostics, and the treatment of CRC.

Material and methods: Specimens were collected from 28 persons (14 female and 14 male), who were operated for CRC. The molecular analysis was performed using oligonucleotide microarrays, mRNA used was collected from adenocarcinoma, and macroscopically healthy tissue. The results were validated using qRT-PCR technique.

Results: Agglomerative hierarchical clustering of normalized mRNA levels has shown 4 groups with statistically different gene expression. The control group was divided into 2 groups, the one was appropriate control (C1), the second (C2) had the genetic properties of the CRC, without pathological changes histologically and macroscopically. The other 2 groups were: LSC (Low stage cancer) and HSC (High stage cancer). Consolidated results of the fluorescency of all of the differential genes, designated two coding E-cadherin (CDH1) with the lower expression, and P-cadherin (CDH3) with higher expression in CRC tissue.

Conclusions: The levels of genes expression are different for several groups of cadherins, and are related with the stage of CRC, therefore could be potentially the useful marker of the stage of the disease, also applicable in treatment and diagnostics of CRC.

No MeSH data available.


Related in: MedlinePlus

Variations in the profile of mRNA expression of cadherins selected as differentiating cadherins, designated by oligonucleotide microarrays. (A) CDH1, (B) CDH3, (C) CDH5, (D) CDH13.
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f3-medscimonit-21-2031: Variations in the profile of mRNA expression of cadherins selected as differentiating cadherins, designated by oligonucleotide microarrays. (A) CDH1, (B) CDH3, (C) CDH5, (D) CDH13.

Mentions: The results show that the profile of 28 cadherin mRNA concentrations changes depending on the stage of disease progression. However, we still did not know whether the observed differences were statistically significant. Therefore, we used analysis of variance (ANOVA), which showed that for 28 cadherin ID mRNA concentrations, statistically significant differences were observed for 4 cadherin mRNA concentrations, assuming p<0.05 and FC parameter >2 (log2). To find out which groups of transcriptomes differentiate the indicated genes, we conducted the Tukey’s HSD post hoc test to obtain the specific number of ID mRNA differentiating the analyzed groups (Table 2, Figure 3).


Expression Level of Genes Coding for Cell Adhesion Molecules of Cadherin Group in Colorectal Cancer Patients.

Lorenc Z, Opiłka MN, Kruszniewska-Rajs C, Rajs A, Waniczek D, Starzewska M, Lorenc J, Mazurek U - Med. Sci. Monit. (2015)

Variations in the profile of mRNA expression of cadherins selected as differentiating cadherins, designated by oligonucleotide microarrays. (A) CDH1, (B) CDH3, (C) CDH5, (D) CDH13.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4514365&req=5

f3-medscimonit-21-2031: Variations in the profile of mRNA expression of cadherins selected as differentiating cadherins, designated by oligonucleotide microarrays. (A) CDH1, (B) CDH3, (C) CDH5, (D) CDH13.
Mentions: The results show that the profile of 28 cadherin mRNA concentrations changes depending on the stage of disease progression. However, we still did not know whether the observed differences were statistically significant. Therefore, we used analysis of variance (ANOVA), which showed that for 28 cadherin ID mRNA concentrations, statistically significant differences were observed for 4 cadherin mRNA concentrations, assuming p<0.05 and FC parameter >2 (log2). To find out which groups of transcriptomes differentiate the indicated genes, we conducted the Tukey’s HSD post hoc test to obtain the specific number of ID mRNA differentiating the analyzed groups (Table 2, Figure 3).

Bottom Line: The results were validated using qRT-PCR technique.Agglomerative hierarchical clustering of normalized mRNA levels has shown 4 groups with statistically different gene expression.Consolidated results of the fluorescency of all of the differential genes, designated two coding E-cadherin (CDH1) with the lower expression, and P-cadherin (CDH3) with higher expression in CRC tissue.

View Article: PubMed Central - PubMed

Affiliation: Chair and Clinical Department of General, Colorectal and Trauma Surgery, Medical University of Silesia, Katowice, Poland.

ABSTRACT

Background: Colorectal Cancer (CRC) is one of the most frequently diagnosed neoplasms and also one of the main death causes. Cell adhesion molecules are taking part in specific junctions, contributing to tissue integrality. Lower expression of the cadherins may be correlated with poorer differentiation of the CRC, and its more aggressive phenotype. The aim of the study is to designate the cadherin genes potentially useful for the diagnostics, prognostics, and the treatment of CRC.

Material and methods: Specimens were collected from 28 persons (14 female and 14 male), who were operated for CRC. The molecular analysis was performed using oligonucleotide microarrays, mRNA used was collected from adenocarcinoma, and macroscopically healthy tissue. The results were validated using qRT-PCR technique.

Results: Agglomerative hierarchical clustering of normalized mRNA levels has shown 4 groups with statistically different gene expression. The control group was divided into 2 groups, the one was appropriate control (C1), the second (C2) had the genetic properties of the CRC, without pathological changes histologically and macroscopically. The other 2 groups were: LSC (Low stage cancer) and HSC (High stage cancer). Consolidated results of the fluorescency of all of the differential genes, designated two coding E-cadherin (CDH1) with the lower expression, and P-cadherin (CDH3) with higher expression in CRC tissue.

Conclusions: The levels of genes expression are different for several groups of cadherins, and are related with the stage of CRC, therefore could be potentially the useful marker of the stage of the disease, also applicable in treatment and diagnostics of CRC.

No MeSH data available.


Related in: MedlinePlus