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Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening.

Guo Y, Hu B, Tang C, Wu Y, Sun P, Zhang X, Jia Y - Int J Nanomedicine (2015)

Bottom Line: Its ability to induce new bone formation was evaluated using an in vivo animal model.We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface.Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

ABSTRACT
Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.

No MeSH data available.


Related in: MedlinePlus

Fluorescence microscopy images of cells double stained with phalloidin for actin filaments (red) and DAPI for nuclei (blue) on Ti (A) and USP-Ti (B) samples.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; Ti, titanium.
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f7-ijn-10-4593: Fluorescence microscopy images of cells double stained with phalloidin for actin filaments (red) and DAPI for nuclei (blue) on Ti (A) and USP-Ti (B) samples.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; Ti, titanium.

Mentions: Figure 7 shows the morphology of osteoblasts examined by CLSM with dual staining of actin filaments and nuclei, and Figure 8 shows the SEM images of osteoblasts after culture for 12 hours on the surfaces of the Ti and USP-Ti samples. It is obvious that the osteoblasts had spread well. Actin filaments were clearly observed on both samples.


Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening.

Guo Y, Hu B, Tang C, Wu Y, Sun P, Zhang X, Jia Y - Int J Nanomedicine (2015)

Fluorescence microscopy images of cells double stained with phalloidin for actin filaments (red) and DAPI for nuclei (blue) on Ti (A) and USP-Ti (B) samples.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; Ti, titanium.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4514313&req=5

f7-ijn-10-4593: Fluorescence microscopy images of cells double stained with phalloidin for actin filaments (red) and DAPI for nuclei (blue) on Ti (A) and USP-Ti (B) samples.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; Ti, titanium.
Mentions: Figure 7 shows the morphology of osteoblasts examined by CLSM with dual staining of actin filaments and nuclei, and Figure 8 shows the SEM images of osteoblasts after culture for 12 hours on the surfaces of the Ti and USP-Ti samples. It is obvious that the osteoblasts had spread well. Actin filaments were clearly observed on both samples.

Bottom Line: Its ability to induce new bone formation was evaluated using an in vivo animal model.We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface.Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

ABSTRACT
Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.

No MeSH data available.


Related in: MedlinePlus