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Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening.

Guo Y, Hu B, Tang C, Wu Y, Sun P, Zhang X, Jia Y - Int J Nanomedicine (2015)

Bottom Line: Surface topography has significant influence on good and fast osseointegration of biomedical implants.Its ability to induce new bone formation was evaluated using an in vivo animal model.Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

ABSTRACT
Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.

No MeSH data available.


Related in: MedlinePlus

XRD patterns of the USP-Ti surface.Abbreviations: Ti, titanium; XRD, X-ray diffraction; USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; au,.
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f3-ijn-10-4593: XRD patterns of the USP-Ti surface.Abbreviations: Ti, titanium; XRD, X-ray diffraction; USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; au,.

Mentions: The SEM micrograph of the USP-Ti surface is presented in Figure 2, and it is obvious that many pits can be seen on the surface of USP-Ti, and these are generated when the surface was struck by the spherical shots. The XRD pattern of USP-Ti is shown in Figure 3. The average size of the grains was in the range of 57–88 nm, with the method of Scherrer.18 The Ra of the Ti and USP-Ti samples determined by AFM (Figure 4) is summarized in Table 1, and the roughness of USP-Ti was found to be higher than that of Ti. The contact angles of the water droplets on Ti and USP-Ti are also listed in Table 1, indicating that USP-Ti surface exhibits better hydrophilicity than the Ti surface.


Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening.

Guo Y, Hu B, Tang C, Wu Y, Sun P, Zhang X, Jia Y - Int J Nanomedicine (2015)

XRD patterns of the USP-Ti surface.Abbreviations: Ti, titanium; XRD, X-ray diffraction; USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; au,.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4514313&req=5

f3-ijn-10-4593: XRD patterns of the USP-Ti surface.Abbreviations: Ti, titanium; XRD, X-ray diffraction; USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; au,.
Mentions: The SEM micrograph of the USP-Ti surface is presented in Figure 2, and it is obvious that many pits can be seen on the surface of USP-Ti, and these are generated when the surface was struck by the spherical shots. The XRD pattern of USP-Ti is shown in Figure 3. The average size of the grains was in the range of 57–88 nm, with the method of Scherrer.18 The Ra of the Ti and USP-Ti samples determined by AFM (Figure 4) is summarized in Table 1, and the roughness of USP-Ti was found to be higher than that of Ti. The contact angles of the water droplets on Ti and USP-Ti are also listed in Table 1, indicating that USP-Ti surface exhibits better hydrophilicity than the Ti surface.

Bottom Line: Surface topography has significant influence on good and fast osseointegration of biomedical implants.Its ability to induce new bone formation was evaluated using an in vivo animal model.Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

ABSTRACT
Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.

No MeSH data available.


Related in: MedlinePlus