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Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening.

Guo Y, Hu B, Tang C, Wu Y, Sun P, Zhang X, Jia Y - Int J Nanomedicine (2015)

Bottom Line: Surface topography has significant influence on good and fast osseointegration of biomedical implants.Its ability to induce new bone formation was evaluated using an in vivo animal model.Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

ABSTRACT
Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.

No MeSH data available.


Related in: MedlinePlus

The surface BAR (%) and NBA (mm2) of Ti and USP-Ti in the proximal, middle, and distal sections at 12 weeks.Notes: Data are shown as the mean ± SD. *P<0.05 compared with Ti.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; BAR, bone apposition ratio; NBA, new bone area; SD, standard deviation; Ti, titanium.
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f13-ijn-10-4593: The surface BAR (%) and NBA (mm2) of Ti and USP-Ti in the proximal, middle, and distal sections at 12 weeks.Notes: Data are shown as the mean ± SD. *P<0.05 compared with Ti.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; BAR, bone apposition ratio; NBA, new bone area; SD, standard deviation; Ti, titanium.

Mentions: The representative histological images of the middle section of the USP-Ti at 12 weeks after implantation are shown in Figure 12. Newly formed bone tissue was clearly observed around the Ti and USP-Ti surfaces. As shown in Figure 12, good contact was seen between the bone tissue and the USP-Ti surface. We can see a double line of tetracycline and calcein clearly under fluorescence microscopy (Figures 12B and 12D), suggesting new bone formation. The BAR and NBA at the surface of the USP-Ti was higher than that on Ti, which indicates that the in vivo osseointegration of USP-Ti was better than that of Ti (Figure 13).


Increased osteoblast function in vitro and in vivo through surface nanostructuring by ultrasonic shot peening.

Guo Y, Hu B, Tang C, Wu Y, Sun P, Zhang X, Jia Y - Int J Nanomedicine (2015)

The surface BAR (%) and NBA (mm2) of Ti and USP-Ti in the proximal, middle, and distal sections at 12 weeks.Notes: Data are shown as the mean ± SD. *P<0.05 compared with Ti.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; BAR, bone apposition ratio; NBA, new bone area; SD, standard deviation; Ti, titanium.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4514313&req=5

f13-ijn-10-4593: The surface BAR (%) and NBA (mm2) of Ti and USP-Ti in the proximal, middle, and distal sections at 12 weeks.Notes: Data are shown as the mean ± SD. *P<0.05 compared with Ti.Abbreviations: USP-Ti, Ti surface subjected to USP; USP, ultrasonic shot peening; BAR, bone apposition ratio; NBA, new bone area; SD, standard deviation; Ti, titanium.
Mentions: The representative histological images of the middle section of the USP-Ti at 12 weeks after implantation are shown in Figure 12. Newly formed bone tissue was clearly observed around the Ti and USP-Ti surfaces. As shown in Figure 12, good contact was seen between the bone tissue and the USP-Ti surface. We can see a double line of tetracycline and calcein clearly under fluorescence microscopy (Figures 12B and 12D), suggesting new bone formation. The BAR and NBA at the surface of the USP-Ti was higher than that on Ti, which indicates that the in vivo osseointegration of USP-Ti was better than that of Ti (Figure 13).

Bottom Line: Surface topography has significant influence on good and fast osseointegration of biomedical implants.Its ability to induce new bone formation was evaluated using an in vivo animal model.Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Department, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

ABSTRACT
Surface topography has significant influence on good and fast osseointegration of biomedical implants. In this work, ultrasonic shot peening was conducted to modify titanium to produce nanograined (NG) surface. Its ability to induce new bone formation was evaluated using an in vivo animal model. We demonstrated that the NG surface enhanced osteoblast adhesion, proliferation, differentiation, and mineralization in in vitro experiments compared to coarse-grained titanium surface. Push-out test, histological observations, fluorescent labeling, and histomorphometrical analysis consistently indicated that the NG surfaces developed have the higher osseointegration than coarse-grained surfaces. Those results suggest that ultrasonic shot peening has the potential for future use as a surface modification method in biomedical application.

No MeSH data available.


Related in: MedlinePlus