Limits...
Evaluation of Lactobacillus and Streptococcus mutans by Addition of Probiotics in the form of Curd in the Diet.

Sidhu GK, Mantha S, Murthi S, Sura H, Kadaru P, Jangra JK - J Int Oral Health (2015)

Bottom Line: Probiotics are defined as live microorganisms which when taken in adequate amounts provide a health benefit on the host.In experimental group, 87% children showed decrease and 7% showed increase in S. mutans count (P = 0.83), whereas 67% showed increase and 7% decrease in Lactobacilli count (P = 0.002) after 1-year of follow-up.Efforts should be made to increase the knowledge of the general dental practitioners with this facet of oral disease therapy and promote the implementation of the concept of "food rather than medicine."

View Article: PubMed Central - PubMed

Affiliation: Senior Lecturer, Department of Oral Pathology and Microbiology, Maharaja Ganga Singh Dental College and Research Centre, Sriganganagar, Rajasthan, India.

ABSTRACT

Background: Probiotics are defined as live microorganisms which when taken in adequate amounts provide a health benefit on the host. They have been used to improve gastrointestinal health, and their popularity has prompted increased interest for their role in the promotion of oral health also. The study was undertaken to evaluate the influence of diet alteration on oral microflora with the addition of probiotics in the form of curd in the diet.

Materials and methods: Sample pool included total 20 children, 15 in experimental group; who were given curd containing probiotic bacteria in their diet daily and 5 children in control group; who were contradicted of food containing probiotics. All the children were followed for over a period of 1-year. A pre and post quantitative analysis of Streptococcus mutans and Lactobacilli were done in the saliva of both active and control groups, and caries score was recorded.

Statistical analysis used: The data thus compiled were statistically evaluated using Chi-square test and Pearson correlation on SPSS software version 14.

Results: In experimental group, 87% children showed decrease and 7% showed increase in S. mutans count (P = 0.83), whereas 67% showed increase and 7% decrease in Lactobacilli count (P = 0.002) after 1-year of follow-up.

Conclusions: Probiotics are upcoming as an intriguing field in oral health. Efforts should be made to increase the knowledge of the general dental practitioners with this facet of oral disease therapy and promote the implementation of the concept of "food rather than medicine."

No MeSH data available.


Related in: MedlinePlus

Dentocult Lactobacilli strip kit and procedure for sample collection.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4513783&req=5

Figure 3: Dentocult Lactobacilli strip kit and procedure for sample collection.

Mentions: To estimate Lactobacilli counts, the early morning saliva samples were collected from all the children. Before taking the samples, children were mandated to avoid eating, drinking and brushing of teeth for 1-2 h. Children were instructed to sit in a relaxed upright position until 1-2 ml of unstimulated whole saliva was collected in the floor of the mouth. They were later asked to spit in a disposable sterile plastic container. Saliva was then poured over the modified rogosa agar surface, screwed tightly, labeled and was placed in an incubator at 37°C for 4 days (Figure 3). The existence of Lactobacillus after incubation was evidenced by white to transparent colonies on the modified Rogosa agar surface. The results were assessed as specified by the manufacturer's guidelines as Class 0: 103 CFU/ml, Class 1: 104 CFU/ml, Class 2: 105 CFU/ml, Class 3: 106 CFU/ml (Figure 4). After 1-year of follow-up, the saliva samples were again collected from both the groups as previously described. All the data thus accumulated were statistically analyzed using Chi-square test and Pearson Correlation on SPSS software version 14.


Evaluation of Lactobacillus and Streptococcus mutans by Addition of Probiotics in the form of Curd in the Diet.

Sidhu GK, Mantha S, Murthi S, Sura H, Kadaru P, Jangra JK - J Int Oral Health (2015)

Dentocult Lactobacilli strip kit and procedure for sample collection.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4513783&req=5

Figure 3: Dentocult Lactobacilli strip kit and procedure for sample collection.
Mentions: To estimate Lactobacilli counts, the early morning saliva samples were collected from all the children. Before taking the samples, children were mandated to avoid eating, drinking and brushing of teeth for 1-2 h. Children were instructed to sit in a relaxed upright position until 1-2 ml of unstimulated whole saliva was collected in the floor of the mouth. They were later asked to spit in a disposable sterile plastic container. Saliva was then poured over the modified rogosa agar surface, screwed tightly, labeled and was placed in an incubator at 37°C for 4 days (Figure 3). The existence of Lactobacillus after incubation was evidenced by white to transparent colonies on the modified Rogosa agar surface. The results were assessed as specified by the manufacturer's guidelines as Class 0: 103 CFU/ml, Class 1: 104 CFU/ml, Class 2: 105 CFU/ml, Class 3: 106 CFU/ml (Figure 4). After 1-year of follow-up, the saliva samples were again collected from both the groups as previously described. All the data thus accumulated were statistically analyzed using Chi-square test and Pearson Correlation on SPSS software version 14.

Bottom Line: Probiotics are defined as live microorganisms which when taken in adequate amounts provide a health benefit on the host.In experimental group, 87% children showed decrease and 7% showed increase in S. mutans count (P = 0.83), whereas 67% showed increase and 7% decrease in Lactobacilli count (P = 0.002) after 1-year of follow-up.Efforts should be made to increase the knowledge of the general dental practitioners with this facet of oral disease therapy and promote the implementation of the concept of "food rather than medicine."

View Article: PubMed Central - PubMed

Affiliation: Senior Lecturer, Department of Oral Pathology and Microbiology, Maharaja Ganga Singh Dental College and Research Centre, Sriganganagar, Rajasthan, India.

ABSTRACT

Background: Probiotics are defined as live microorganisms which when taken in adequate amounts provide a health benefit on the host. They have been used to improve gastrointestinal health, and their popularity has prompted increased interest for their role in the promotion of oral health also. The study was undertaken to evaluate the influence of diet alteration on oral microflora with the addition of probiotics in the form of curd in the diet.

Materials and methods: Sample pool included total 20 children, 15 in experimental group; who were given curd containing probiotic bacteria in their diet daily and 5 children in control group; who were contradicted of food containing probiotics. All the children were followed for over a period of 1-year. A pre and post quantitative analysis of Streptococcus mutans and Lactobacilli were done in the saliva of both active and control groups, and caries score was recorded.

Statistical analysis used: The data thus compiled were statistically evaluated using Chi-square test and Pearson correlation on SPSS software version 14.

Results: In experimental group, 87% children showed decrease and 7% showed increase in S. mutans count (P = 0.83), whereas 67% showed increase and 7% decrease in Lactobacilli count (P = 0.002) after 1-year of follow-up.

Conclusions: Probiotics are upcoming as an intriguing field in oral health. Efforts should be made to increase the knowledge of the general dental practitioners with this facet of oral disease therapy and promote the implementation of the concept of "food rather than medicine."

No MeSH data available.


Related in: MedlinePlus