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Antioxidant activity and hepatoprotective potential of Cedrelopsis grevei on cypermethrin induced oxidative stress and liver damage in male mice.

Mossa AT, Heikal TM, Belaiba M, Raoelison EG, Ferhout H, Bouajila J - BMC Complement Altern Med (2015)

Bottom Line: Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes.The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Pesticide Chemistry Department, National Research Centre (NRC), 33 El Bohouth Street (former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt. abdeltawab.mossa@yahoo.com.

ABSTRACT

Background: The liver is the most sensitive and main target organ of pesticide toxicity and damage, they play an essential role in metabolism and detoxification of pesticides. Due to these functions, hepatotoxicity continues to be among the main threats to public health, and they remain problems throughout the world. Therefore, the present study was designed to evaluate the antioxidant and hepatoprotective effects of Cedrelopsis grevei leaves against cypermethrin (Cyp) induced oxidative stress and liver damage in male mice.

Methods: The extracts were subjected to different analyses (phenolics, tannin, flavonoids, antioxidant activity and reducing power assays). For hepatoprotective evaluation, male mice were daily exposed to Cyp and/or C. grevei by gavages for 28 days. Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.

Results: The antioxidant activity of C. grevei methanolic extract was the highest with an IC50<225 μg/ml by DPPH assay. The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes. Histopathological examination in the liver tissue of those mice, demonstrated that a co-administration of methanolic extract (150 & 300 mg/kg/day) showed marked improvement in its histological structure in comparison to Cyp-treated group alone and represented by nil to moderate degree in inflammatory cells.

Conclusions: In view of the data of the present study, it can deduce that cypermethrin caused oxidative damage and liver dysfunction in male mice. C. grevei extract has protective effects on cypermethrin-induced lipid peroxidation, oxidative stress and liver damage. Results indicated that administration of C. grevei is useful, easy, and economical to protect humans against pesticide toxicity. The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts. In a future study, we will identify and investigate the components responsible for the hepatoprotective and antioxidant activities of C. grevei.

No MeSH data available.


Related in: MedlinePlus

Paraffin sections of liver stained by haematoxylin and eosin (H&E) for histopathological changes. Control group and extract groups [A] showing the normal histological structure of the central vein (CV) and surrounding hepatocytes (H) (x40). Cypermethrin group showing [B] degeneration in hepatocytes and dilation of portal vein with inflammatory cells infiltration (m) in the portal area (x80). Cypermethrin plus extract at 150 mg/kg. b.wt. [C] showing dilatation of central vein (CV) ballooning degeneration (h) in surrounding hepatocytes (x80). Cypermethrin plus extract at 300 mg/kg. b.wt. [D] showing dilatation of portal vein (x80)
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Fig5: Paraffin sections of liver stained by haematoxylin and eosin (H&E) for histopathological changes. Control group and extract groups [A] showing the normal histological structure of the central vein (CV) and surrounding hepatocytes (H) (x40). Cypermethrin group showing [B] degeneration in hepatocytes and dilation of portal vein with inflammatory cells infiltration (m) in the portal area (x80). Cypermethrin plus extract at 150 mg/kg. b.wt. [C] showing dilatation of central vein (CV) ballooning degeneration (h) in surrounding hepatocytes (x80). Cypermethrin plus extract at 300 mg/kg. b.wt. [D] showing dilatation of portal vein (x80)

Mentions: The representative picutures of histopathological examination of the liver tissue are shown in Fig. 5 (A-D) and the semi quantitative histological scoring of liver damage is presented in Table 5. Liver sections from the control group mice showed normal hepatic cytoarchitecture. They formed of hepatocytes radiating from central vein to the periphery of the lobules (Fig. 5A). Liver lobules of Cyp-treated mice showed severe degeneration in hepatocytes, necroses, dilation of portal vein with inflammatory cell infiltration in the portal area (Fig. 5B). Liver sections of mice treated with Cyp + Ext 150 (Fig. 5C) showed mild to moderate dilatation of central vein and ballooning degeneration in surrounding hepatocytes. Whereas, in cypermethrin + Ext 300 treated mice (Fig. 5C) showed only moderate dilatation of portal vein.Fig. 5


Antioxidant activity and hepatoprotective potential of Cedrelopsis grevei on cypermethrin induced oxidative stress and liver damage in male mice.

Mossa AT, Heikal TM, Belaiba M, Raoelison EG, Ferhout H, Bouajila J - BMC Complement Altern Med (2015)

Paraffin sections of liver stained by haematoxylin and eosin (H&E) for histopathological changes. Control group and extract groups [A] showing the normal histological structure of the central vein (CV) and surrounding hepatocytes (H) (x40). Cypermethrin group showing [B] degeneration in hepatocytes and dilation of portal vein with inflammatory cells infiltration (m) in the portal area (x80). Cypermethrin plus extract at 150 mg/kg. b.wt. [C] showing dilatation of central vein (CV) ballooning degeneration (h) in surrounding hepatocytes (x80). Cypermethrin plus extract at 300 mg/kg. b.wt. [D] showing dilatation of portal vein (x80)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4513628&req=5

Fig5: Paraffin sections of liver stained by haematoxylin and eosin (H&E) for histopathological changes. Control group and extract groups [A] showing the normal histological structure of the central vein (CV) and surrounding hepatocytes (H) (x40). Cypermethrin group showing [B] degeneration in hepatocytes and dilation of portal vein with inflammatory cells infiltration (m) in the portal area (x80). Cypermethrin plus extract at 150 mg/kg. b.wt. [C] showing dilatation of central vein (CV) ballooning degeneration (h) in surrounding hepatocytes (x80). Cypermethrin plus extract at 300 mg/kg. b.wt. [D] showing dilatation of portal vein (x80)
Mentions: The representative picutures of histopathological examination of the liver tissue are shown in Fig. 5 (A-D) and the semi quantitative histological scoring of liver damage is presented in Table 5. Liver sections from the control group mice showed normal hepatic cytoarchitecture. They formed of hepatocytes radiating from central vein to the periphery of the lobules (Fig. 5A). Liver lobules of Cyp-treated mice showed severe degeneration in hepatocytes, necroses, dilation of portal vein with inflammatory cell infiltration in the portal area (Fig. 5B). Liver sections of mice treated with Cyp + Ext 150 (Fig. 5C) showed mild to moderate dilatation of central vein and ballooning degeneration in surrounding hepatocytes. Whereas, in cypermethrin + Ext 300 treated mice (Fig. 5C) showed only moderate dilatation of portal vein.Fig. 5

Bottom Line: Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes.The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Pesticide Chemistry Department, National Research Centre (NRC), 33 El Bohouth Street (former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt. abdeltawab.mossa@yahoo.com.

ABSTRACT

Background: The liver is the most sensitive and main target organ of pesticide toxicity and damage, they play an essential role in metabolism and detoxification of pesticides. Due to these functions, hepatotoxicity continues to be among the main threats to public health, and they remain problems throughout the world. Therefore, the present study was designed to evaluate the antioxidant and hepatoprotective effects of Cedrelopsis grevei leaves against cypermethrin (Cyp) induced oxidative stress and liver damage in male mice.

Methods: The extracts were subjected to different analyses (phenolics, tannin, flavonoids, antioxidant activity and reducing power assays). For hepatoprotective evaluation, male mice were daily exposed to Cyp and/or C. grevei by gavages for 28 days. Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.

Results: The antioxidant activity of C. grevei methanolic extract was the highest with an IC50<225 μg/ml by DPPH assay. The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes. Histopathological examination in the liver tissue of those mice, demonstrated that a co-administration of methanolic extract (150 & 300 mg/kg/day) showed marked improvement in its histological structure in comparison to Cyp-treated group alone and represented by nil to moderate degree in inflammatory cells.

Conclusions: In view of the data of the present study, it can deduce that cypermethrin caused oxidative damage and liver dysfunction in male mice. C. grevei extract has protective effects on cypermethrin-induced lipid peroxidation, oxidative stress and liver damage. Results indicated that administration of C. grevei is useful, easy, and economical to protect humans against pesticide toxicity. The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts. In a future study, we will identify and investigate the components responsible for the hepatoprotective and antioxidant activities of C. grevei.

No MeSH data available.


Related in: MedlinePlus