Limits...
Antioxidant activity and hepatoprotective potential of Cedrelopsis grevei on cypermethrin induced oxidative stress and liver damage in male mice.

Mossa AT, Heikal TM, Belaiba M, Raoelison EG, Ferhout H, Bouajila J - BMC Complement Altern Med (2015)

Bottom Line: Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes.The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Pesticide Chemistry Department, National Research Centre (NRC), 33 El Bohouth Street (former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt. abdeltawab.mossa@yahoo.com.

ABSTRACT

Background: The liver is the most sensitive and main target organ of pesticide toxicity and damage, they play an essential role in metabolism and detoxification of pesticides. Due to these functions, hepatotoxicity continues to be among the main threats to public health, and they remain problems throughout the world. Therefore, the present study was designed to evaluate the antioxidant and hepatoprotective effects of Cedrelopsis grevei leaves against cypermethrin (Cyp) induced oxidative stress and liver damage in male mice.

Methods: The extracts were subjected to different analyses (phenolics, tannin, flavonoids, antioxidant activity and reducing power assays). For hepatoprotective evaluation, male mice were daily exposed to Cyp and/or C. grevei by gavages for 28 days. Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.

Results: The antioxidant activity of C. grevei methanolic extract was the highest with an IC50<225 μg/ml by DPPH assay. The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes. Histopathological examination in the liver tissue of those mice, demonstrated that a co-administration of methanolic extract (150 & 300 mg/kg/day) showed marked improvement in its histological structure in comparison to Cyp-treated group alone and represented by nil to moderate degree in inflammatory cells.

Conclusions: In view of the data of the present study, it can deduce that cypermethrin caused oxidative damage and liver dysfunction in male mice. C. grevei extract has protective effects on cypermethrin-induced lipid peroxidation, oxidative stress and liver damage. Results indicated that administration of C. grevei is useful, easy, and economical to protect humans against pesticide toxicity. The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts. In a future study, we will identify and investigate the components responsible for the hepatoprotective and antioxidant activities of C. grevei.

No MeSH data available.


Related in: MedlinePlus

Reducing power (absorbance at 700 nm) of MeOH and EthOH extracts of C. grevei. Values are mean ± SD
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4513628&req=5

Fig3: Reducing power (absorbance at 700 nm) of MeOH and EthOH extracts of C. grevei. Values are mean ± SD

Mentions: Figure 1 illustrates a decrease in the concentration of DPPH radical due to the scavenging ability of C. grevei extracts. On DPPH radical, EtOH and MeOH had scavenging effects with increasing concentration in the range of 95–400 μg/mL, and the scavenging effect of EtOH was lower than MeOH. The IC50 value of ascorbic acid was 4.7 μg/mL. MeOH extract (400 μg/mL) exhibited the highest inhibition of about 69.45 %, but this is lower in EtOH extract whose percentage of inhibition is 61.27 %. ABTS assay is shown in Fig. 2. C. grevei extracts (100 μg/mL) exhibited the highest inhibition of 76.81 % and 78.35 % of MeOH and EtOH extracts. As illustrated in Fig. 3, Fe3+ was transformed to Fe2+ in the presence of MeOH and EtOH extracts of C. grevei to measure the reductive capability. C. grevei extracts had significant inhibition of reducing power with increasing concentration in the range of 0.10-0.125 mg/mL.Fig. 1


Antioxidant activity and hepatoprotective potential of Cedrelopsis grevei on cypermethrin induced oxidative stress and liver damage in male mice.

Mossa AT, Heikal TM, Belaiba M, Raoelison EG, Ferhout H, Bouajila J - BMC Complement Altern Med (2015)

Reducing power (absorbance at 700 nm) of MeOH and EthOH extracts of C. grevei. Values are mean ± SD
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4513628&req=5

Fig3: Reducing power (absorbance at 700 nm) of MeOH and EthOH extracts of C. grevei. Values are mean ± SD
Mentions: Figure 1 illustrates a decrease in the concentration of DPPH radical due to the scavenging ability of C. grevei extracts. On DPPH radical, EtOH and MeOH had scavenging effects with increasing concentration in the range of 95–400 μg/mL, and the scavenging effect of EtOH was lower than MeOH. The IC50 value of ascorbic acid was 4.7 μg/mL. MeOH extract (400 μg/mL) exhibited the highest inhibition of about 69.45 %, but this is lower in EtOH extract whose percentage of inhibition is 61.27 %. ABTS assay is shown in Fig. 2. C. grevei extracts (100 μg/mL) exhibited the highest inhibition of 76.81 % and 78.35 % of MeOH and EtOH extracts. As illustrated in Fig. 3, Fe3+ was transformed to Fe2+ in the presence of MeOH and EtOH extracts of C. grevei to measure the reductive capability. C. grevei extracts had significant inhibition of reducing power with increasing concentration in the range of 0.10-0.125 mg/mL.Fig. 1

Bottom Line: Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes.The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts.

View Article: PubMed Central - PubMed

Affiliation: Environmental Toxicology Research Unit (ETRU), Pesticide Chemistry Department, National Research Centre (NRC), 33 El Bohouth Street (former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt. abdeltawab.mossa@yahoo.com.

ABSTRACT

Background: The liver is the most sensitive and main target organ of pesticide toxicity and damage, they play an essential role in metabolism and detoxification of pesticides. Due to these functions, hepatotoxicity continues to be among the main threats to public health, and they remain problems throughout the world. Therefore, the present study was designed to evaluate the antioxidant and hepatoprotective effects of Cedrelopsis grevei leaves against cypermethrin (Cyp) induced oxidative stress and liver damage in male mice.

Methods: The extracts were subjected to different analyses (phenolics, tannin, flavonoids, antioxidant activity and reducing power assays). For hepatoprotective evaluation, male mice were daily exposed to Cyp and/or C. grevei by gavages for 28 days. Hepatoprotective effects were demonstrated by significant alterations in serum liver dysfunction biomarker enzymes, liver lipid peroxidation and antioxidant enzymes.

Results: The antioxidant activity of C. grevei methanolic extract was the highest with an IC50<225 μg/ml by DPPH assay. The high dose of methanolic extract (300 mg/kg. b.wt.) was effective to attenuate the perturbations in the tested enzymes. Histopathological examination in the liver tissue of those mice, demonstrated that a co-administration of methanolic extract (150 & 300 mg/kg/day) showed marked improvement in its histological structure in comparison to Cyp-treated group alone and represented by nil to moderate degree in inflammatory cells.

Conclusions: In view of the data of the present study, it can deduce that cypermethrin caused oxidative damage and liver dysfunction in male mice. C. grevei extract has protective effects on cypermethrin-induced lipid peroxidation, oxidative stress and liver damage. Results indicated that administration of C. grevei is useful, easy, and economical to protect humans against pesticide toxicity. The results presented here can be considered as the first information on the hepatoprotective and antioxidant properties of C. grevei extracts. In a future study, we will identify and investigate the components responsible for the hepatoprotective and antioxidant activities of C. grevei.

No MeSH data available.


Related in: MedlinePlus