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Developmental succession of the microbiome of Culex mosquitoes.

Duguma D, Hall MW, Rugman-Jones P, Stouthamer R, Terenius O, Neufeld JD, Walton WE - BMC Microbiol. (2015)

Bottom Line: Bacterial communities in early instar C. tarsalis larvae were significantly more diverse when compared to late instar larvae, pupae and newly emerged adults.Some of the bacterial OTUs found in the early instar larvae were also found across developmental stages.Differences in microbiota observed in larval habitats did not influence bacterial community profiles of late instar larvae or adults.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of California Riverside, Riverside, CA, 92521, USA. duguma@ufl.edu.

ABSTRACT

Background: The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that bacteria found in the larval stage of Culex are transstadially transmitted to the adult stage, and to compare the microbiomes of field-collected versus laboratory-reared mosquitoes.

Results: Beta diversity analysis revealed that bacterial community structure differed among three life stages (larvae, pupae and adults) of Culex tarsalis. Although only ~2% of the total number of bacterial OTUs were found in all stages, sequences from these OTUs accounted for nearly 82% of the total bacterial sequences recovered from all stages. Thorsellia (Gammaproteobacteria) was the most abundant bacterial taxon found across all developmental stages of field-collected Culex mosquitoes, but was rare in mosquitoes from laboratory-reared colonies. The proportion of Thorsellia sequences in the microbiomes of mosquito life stages varied ontogenetically with the greatest proportions recovered from the pupae of C. tarsalis and the lowest from newly emerged adults. The microbiome of field-collected late instar larvae was not influenced significantly by differences in the microbiota of the habitat due to habitat age or biopesticide treatments. The microbiome diversity was the greatest in the early instar larvae and the lowest in laboratory-reared mosquitoes.

Conclusions: Bacterial communities in early instar C. tarsalis larvae were significantly more diverse when compared to late instar larvae, pupae and newly emerged adults. Some of the bacterial OTUs found in the early instar larvae were also found across developmental stages. Thorsellia dominated the bacterial communities in field-collected immature stages but occurred at much lower relative abundance in adults. Differences in microbiota observed in larval habitats did not influence bacterial community profiles of late instar larvae or adults. However, bacterial communities in laboratory-reared C. tarsalis larvae differed significantly from the field. Determining the role of Thorsellia in mosquitoes and its distribution across different species of mosquitoes warrants further investigation.

No MeSH data available.


Related in: MedlinePlus

Family-level abundance of bacterial communities. Family-level abundance (%) of bacterial communities in field-collected early (1st and 2nd) instar larvae, late (3rd and 4th) instar larvae, pupae and adults of C. tarsalis. Only sequences classified to family level were included. Because the treatments effects on the gut bacterial community structure within each stage were not significantly different, mosquitoes from all treatments were included in this figure
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Fig3: Family-level abundance of bacterial communities. Family-level abundance (%) of bacterial communities in field-collected early (1st and 2nd) instar larvae, late (3rd and 4th) instar larvae, pupae and adults of C. tarsalis. Only sequences classified to family level were included. Because the treatments effects on the gut bacterial community structure within each stage were not significantly different, mosquitoes from all treatments were included in this figure

Mentions: A total of 5,888 OTUs (805,169 sequences from 7 samples) in 34 bacterial phyla were recovered from early (1st and 2nd) instars of Culex larvae. Proteobacteria (73 %), Firmicutes (18 %), and Bacteroidetes (7 %) dominated the early instar Culex larvae (Additional file 1: Table S1). Overall, bacterial taxa in Gammaproteobacteria (43 %), Betaprotobacteria (26 %) and Bacilli (13 %) were the three most abundant classes found in the early stages of the mosquito life cycle (Additional file 1: Table S1). Thorselliaceae (27.2 %) and Comamonadaceae (21.2 %) were the two most abundant families found in early instar larvae (Fig. 3). Thorsellia was the most abundant (19 %) genus found in the early stages followed by an unclassified taxon of Gammaproteobacteria (8.8 %) and Aeromonas (7 %) (Additional file 3: Table S3).Fig. 3


Developmental succession of the microbiome of Culex mosquitoes.

Duguma D, Hall MW, Rugman-Jones P, Stouthamer R, Terenius O, Neufeld JD, Walton WE - BMC Microbiol. (2015)

Family-level abundance of bacterial communities. Family-level abundance (%) of bacterial communities in field-collected early (1st and 2nd) instar larvae, late (3rd and 4th) instar larvae, pupae and adults of C. tarsalis. Only sequences classified to family level were included. Because the treatments effects on the gut bacterial community structure within each stage were not significantly different, mosquitoes from all treatments were included in this figure
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4513620&req=5

Fig3: Family-level abundance of bacterial communities. Family-level abundance (%) of bacterial communities in field-collected early (1st and 2nd) instar larvae, late (3rd and 4th) instar larvae, pupae and adults of C. tarsalis. Only sequences classified to family level were included. Because the treatments effects on the gut bacterial community structure within each stage were not significantly different, mosquitoes from all treatments were included in this figure
Mentions: A total of 5,888 OTUs (805,169 sequences from 7 samples) in 34 bacterial phyla were recovered from early (1st and 2nd) instars of Culex larvae. Proteobacteria (73 %), Firmicutes (18 %), and Bacteroidetes (7 %) dominated the early instar Culex larvae (Additional file 1: Table S1). Overall, bacterial taxa in Gammaproteobacteria (43 %), Betaprotobacteria (26 %) and Bacilli (13 %) were the three most abundant classes found in the early stages of the mosquito life cycle (Additional file 1: Table S1). Thorselliaceae (27.2 %) and Comamonadaceae (21.2 %) were the two most abundant families found in early instar larvae (Fig. 3). Thorsellia was the most abundant (19 %) genus found in the early stages followed by an unclassified taxon of Gammaproteobacteria (8.8 %) and Aeromonas (7 %) (Additional file 3: Table S3).Fig. 3

Bottom Line: Bacterial communities in early instar C. tarsalis larvae were significantly more diverse when compared to late instar larvae, pupae and newly emerged adults.Some of the bacterial OTUs found in the early instar larvae were also found across developmental stages.Differences in microbiota observed in larval habitats did not influence bacterial community profiles of late instar larvae or adults.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of California Riverside, Riverside, CA, 92521, USA. duguma@ufl.edu.

ABSTRACT

Background: The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that bacteria found in the larval stage of Culex are transstadially transmitted to the adult stage, and to compare the microbiomes of field-collected versus laboratory-reared mosquitoes.

Results: Beta diversity analysis revealed that bacterial community structure differed among three life stages (larvae, pupae and adults) of Culex tarsalis. Although only ~2% of the total number of bacterial OTUs were found in all stages, sequences from these OTUs accounted for nearly 82% of the total bacterial sequences recovered from all stages. Thorsellia (Gammaproteobacteria) was the most abundant bacterial taxon found across all developmental stages of field-collected Culex mosquitoes, but was rare in mosquitoes from laboratory-reared colonies. The proportion of Thorsellia sequences in the microbiomes of mosquito life stages varied ontogenetically with the greatest proportions recovered from the pupae of C. tarsalis and the lowest from newly emerged adults. The microbiome of field-collected late instar larvae was not influenced significantly by differences in the microbiota of the habitat due to habitat age or biopesticide treatments. The microbiome diversity was the greatest in the early instar larvae and the lowest in laboratory-reared mosquitoes.

Conclusions: Bacterial communities in early instar C. tarsalis larvae were significantly more diverse when compared to late instar larvae, pupae and newly emerged adults. Some of the bacterial OTUs found in the early instar larvae were also found across developmental stages. Thorsellia dominated the bacterial communities in field-collected immature stages but occurred at much lower relative abundance in adults. Differences in microbiota observed in larval habitats did not influence bacterial community profiles of late instar larvae or adults. However, bacterial communities in laboratory-reared C. tarsalis larvae differed significantly from the field. Determining the role of Thorsellia in mosquitoes and its distribution across different species of mosquitoes warrants further investigation.

No MeSH data available.


Related in: MedlinePlus