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Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis.

Li ZG, Chen HW, Li QT, Tao JJ, Bian XH, Ma B, Zhang WK, Chen SY, Zhang JS - Sci Rep (2015)

Bottom Line: Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity.Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2.These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

ABSTRACT
Ethylene perceived by a family of five receptors regulates many developmental processes in Arabidopsis. Here we conducted the yeast two-hybrid assay to screen for additional unidentified proteins that interact with subfamily II ethylene receptor ETR2. Three SAUR proteins, named SAUR76, 77 and 78, were identified to associate with both ETR2 and EIN4 in different assays. Interaction of SAUR76 and SAUR78 with ETR2 was further verified by co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays. Expressions of SAUR76-78 are induced by auxin and ethylene treatments. Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity. Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2. SAUR76/78 proteins are regulated by 26S proteasome system and larger tag increases their protein stability. These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus

SAUR76 mutation partially suppresses ethylene insensitivity of etr2-2.(a) Comparison of four-day-old dark-grown seedlings germinated in the presence or absence of 10 ppm ethylene. Representative seedlings of double mutant etr2-2saur76 were compared with WT and the single mutant. The etr2-2 is a weak suppressor of etr2-1. (b) Hypocotyl length of four-day-old dark-grown seedlings in response to ethylene. Plants in (a) were used. Bars indicate SD (n = 40). Different letters above each column indicate significant difference between the compared pairs (P < 0.05). (c) Hypocotyl length of four-day-old etiolated seedlings for etr2-1saur76 and ein4-1saur76 double mutants in response to ethylene. Single mutants were also compared. Bars indicate SD (n = 40).
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f8: SAUR76 mutation partially suppresses ethylene insensitivity of etr2-2.(a) Comparison of four-day-old dark-grown seedlings germinated in the presence or absence of 10 ppm ethylene. Representative seedlings of double mutant etr2-2saur76 were compared with WT and the single mutant. The etr2-2 is a weak suppressor of etr2-1. (b) Hypocotyl length of four-day-old dark-grown seedlings in response to ethylene. Plants in (a) were used. Bars indicate SD (n = 40). Different letters above each column indicate significant difference between the compared pairs (P < 0.05). (c) Hypocotyl length of four-day-old etiolated seedlings for etr2-1saur76 and ein4-1saur76 double mutants in response to ethylene. Single mutants were also compared. Bars indicate SD (n = 40).

Mentions: As overexpression of SAUR76 can partially complement the phenotype of ethylene receptor loss-of-function mutant etr2-3ein4-4 (Fig. 7), we tested whether saur76 mutant can suppress the phenotype of ethylene receptor gain-of-function mutant. Double mutants etr2-1saur76, etr2-2saur76 and ein4-1saur76 were generated for examination of ethylene response. The etr2-2 is a weak allele compared to the ethylene insensitivity of etr2-1 in triple response assay30. Double mutant etr2-2saur76 showed shorter hypocotyl than etr2-2 but longer than wild type in presence of 1 and 10 ppm ethylene (Fig. 8a,b). No significant difference of hypocotyl length was observed between etr2-2saur76 and etr2-2 in the absence or presence of 0.1 ppm ethylene (Fig. 8a,b). Double mutant etr2-1saur76 and ein4-1saur76 had nearly the same hypocotyl length as the single mutant etr2-1 and ein4-1 respectivelyin the presence or absence of ethylene (Fig. 8c). All these phenotypes demonstrate that saur76 mutant can partially suppress the phenotype of etr2-2 but not etr2-1 or ein4-1.


Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis.

Li ZG, Chen HW, Li QT, Tao JJ, Bian XH, Ma B, Zhang WK, Chen SY, Zhang JS - Sci Rep (2015)

SAUR76 mutation partially suppresses ethylene insensitivity of etr2-2.(a) Comparison of four-day-old dark-grown seedlings germinated in the presence or absence of 10 ppm ethylene. Representative seedlings of double mutant etr2-2saur76 were compared with WT and the single mutant. The etr2-2 is a weak suppressor of etr2-1. (b) Hypocotyl length of four-day-old dark-grown seedlings in response to ethylene. Plants in (a) were used. Bars indicate SD (n = 40). Different letters above each column indicate significant difference between the compared pairs (P < 0.05). (c) Hypocotyl length of four-day-old etiolated seedlings for etr2-1saur76 and ein4-1saur76 double mutants in response to ethylene. Single mutants were also compared. Bars indicate SD (n = 40).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4513569&req=5

f8: SAUR76 mutation partially suppresses ethylene insensitivity of etr2-2.(a) Comparison of four-day-old dark-grown seedlings germinated in the presence or absence of 10 ppm ethylene. Representative seedlings of double mutant etr2-2saur76 were compared with WT and the single mutant. The etr2-2 is a weak suppressor of etr2-1. (b) Hypocotyl length of four-day-old dark-grown seedlings in response to ethylene. Plants in (a) were used. Bars indicate SD (n = 40). Different letters above each column indicate significant difference between the compared pairs (P < 0.05). (c) Hypocotyl length of four-day-old etiolated seedlings for etr2-1saur76 and ein4-1saur76 double mutants in response to ethylene. Single mutants were also compared. Bars indicate SD (n = 40).
Mentions: As overexpression of SAUR76 can partially complement the phenotype of ethylene receptor loss-of-function mutant etr2-3ein4-4 (Fig. 7), we tested whether saur76 mutant can suppress the phenotype of ethylene receptor gain-of-function mutant. Double mutants etr2-1saur76, etr2-2saur76 and ein4-1saur76 were generated for examination of ethylene response. The etr2-2 is a weak allele compared to the ethylene insensitivity of etr2-1 in triple response assay30. Double mutant etr2-2saur76 showed shorter hypocotyl than etr2-2 but longer than wild type in presence of 1 and 10 ppm ethylene (Fig. 8a,b). No significant difference of hypocotyl length was observed between etr2-2saur76 and etr2-2 in the absence or presence of 0.1 ppm ethylene (Fig. 8a,b). Double mutant etr2-1saur76 and ein4-1saur76 had nearly the same hypocotyl length as the single mutant etr2-1 and ein4-1 respectivelyin the presence or absence of ethylene (Fig. 8c). All these phenotypes demonstrate that saur76 mutant can partially suppress the phenotype of etr2-2 but not etr2-1 or ein4-1.

Bottom Line: Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity.Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2.These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

ABSTRACT
Ethylene perceived by a family of five receptors regulates many developmental processes in Arabidopsis. Here we conducted the yeast two-hybrid assay to screen for additional unidentified proteins that interact with subfamily II ethylene receptor ETR2. Three SAUR proteins, named SAUR76, 77 and 78, were identified to associate with both ETR2 and EIN4 in different assays. Interaction of SAUR76 and SAUR78 with ETR2 was further verified by co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays. Expressions of SAUR76-78 are induced by auxin and ethylene treatments. Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity. Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2. SAUR76/78 proteins are regulated by 26S proteasome system and larger tag increases their protein stability. These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus