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Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis.

Li ZG, Chen HW, Li QT, Tao JJ, Bian XH, Ma B, Zhang WK, Chen SY, Zhang JS - Sci Rep (2015)

Bottom Line: Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity.Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2.These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

ABSTRACT
Ethylene perceived by a family of five receptors regulates many developmental processes in Arabidopsis. Here we conducted the yeast two-hybrid assay to screen for additional unidentified proteins that interact with subfamily II ethylene receptor ETR2. Three SAUR proteins, named SAUR76, 77 and 78, were identified to associate with both ETR2 and EIN4 in different assays. Interaction of SAUR76 and SAUR78 with ETR2 was further verified by co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays. Expressions of SAUR76-78 are induced by auxin and ethylene treatments. Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity. Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2. SAUR76/78 proteins are regulated by 26S proteasome system and larger tag increases their protein stability. These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

No MeSH data available.


Overexpressions of SAUR76-78 promote seedling growth in transgenic Arabidopsis plants.(a) SAUR76-78 expression in SAUR-overexpressing lines by Northern analysis. The rRNA was stained as a loading control. WT: Col-0. (b) Identification of saur76 T-DNA insertion mutant. Solid black box represents the only exon and the position of T-DNA insertion is indicated by triangle. Thesaur76 is a knockout mutant. Expression of SAUR76 relative to Actin is measured by quantitative PCR. (c) Comparison of five-day-old light-grown seedlings and rosettes of 30-day-old light-grown plants for various genotypes. (d) Cotyledon length of seedlings in upper panel of (c). Bars indicate SD (n = 40). Rosette diameter of plants in lower panel of (c). Bars indicate SD (n = 20). (e) Scanning electron micrograph of leaf epidermal cells from 20-day-old plants and comparison of leaf epidermal cell area. RNAi1-49: an RNAi line of SAUR78. nP::SAUR76/saur76: saur76 complemented with SAUR76 genomic sequence driven by native promoter. Right panel: bars indicate SD (n ≥ 20). For (d), (e) and (f), “*” and “**” indicate significant difference compared with WT at P < 0.05 and P < 0.01, respectively.
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f5: Overexpressions of SAUR76-78 promote seedling growth in transgenic Arabidopsis plants.(a) SAUR76-78 expression in SAUR-overexpressing lines by Northern analysis. The rRNA was stained as a loading control. WT: Col-0. (b) Identification of saur76 T-DNA insertion mutant. Solid black box represents the only exon and the position of T-DNA insertion is indicated by triangle. Thesaur76 is a knockout mutant. Expression of SAUR76 relative to Actin is measured by quantitative PCR. (c) Comparison of five-day-old light-grown seedlings and rosettes of 30-day-old light-grown plants for various genotypes. (d) Cotyledon length of seedlings in upper panel of (c). Bars indicate SD (n = 40). Rosette diameter of plants in lower panel of (c). Bars indicate SD (n = 20). (e) Scanning electron micrograph of leaf epidermal cells from 20-day-old plants and comparison of leaf epidermal cell area. RNAi1-49: an RNAi line of SAUR78. nP::SAUR76/saur76: saur76 complemented with SAUR76 genomic sequence driven by native promoter. Right panel: bars indicate SD (n ≥ 20). For (d), (e) and (f), “*” and “**” indicate significant difference compared with WT at P < 0.05 and P < 0.01, respectively.

Mentions: To investigate the biological functions of SAURs in plants, SAUR76-78, driven by 35S cauliflower mosaic virus (CaMV) promoter in pROKII vector, were transformed into Arabidopsis and higher expressors were analyzed (Fig. 5a). A T-DNA insertion mutant of saur76 was also identified as a SAUR76 knockout mutant (Fig. 5b).


Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis.

Li ZG, Chen HW, Li QT, Tao JJ, Bian XH, Ma B, Zhang WK, Chen SY, Zhang JS - Sci Rep (2015)

Overexpressions of SAUR76-78 promote seedling growth in transgenic Arabidopsis plants.(a) SAUR76-78 expression in SAUR-overexpressing lines by Northern analysis. The rRNA was stained as a loading control. WT: Col-0. (b) Identification of saur76 T-DNA insertion mutant. Solid black box represents the only exon and the position of T-DNA insertion is indicated by triangle. Thesaur76 is a knockout mutant. Expression of SAUR76 relative to Actin is measured by quantitative PCR. (c) Comparison of five-day-old light-grown seedlings and rosettes of 30-day-old light-grown plants for various genotypes. (d) Cotyledon length of seedlings in upper panel of (c). Bars indicate SD (n = 40). Rosette diameter of plants in lower panel of (c). Bars indicate SD (n = 20). (e) Scanning electron micrograph of leaf epidermal cells from 20-day-old plants and comparison of leaf epidermal cell area. RNAi1-49: an RNAi line of SAUR78. nP::SAUR76/saur76: saur76 complemented with SAUR76 genomic sequence driven by native promoter. Right panel: bars indicate SD (n ≥ 20). For (d), (e) and (f), “*” and “**” indicate significant difference compared with WT at P < 0.05 and P < 0.01, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4513569&req=5

f5: Overexpressions of SAUR76-78 promote seedling growth in transgenic Arabidopsis plants.(a) SAUR76-78 expression in SAUR-overexpressing lines by Northern analysis. The rRNA was stained as a loading control. WT: Col-0. (b) Identification of saur76 T-DNA insertion mutant. Solid black box represents the only exon and the position of T-DNA insertion is indicated by triangle. Thesaur76 is a knockout mutant. Expression of SAUR76 relative to Actin is measured by quantitative PCR. (c) Comparison of five-day-old light-grown seedlings and rosettes of 30-day-old light-grown plants for various genotypes. (d) Cotyledon length of seedlings in upper panel of (c). Bars indicate SD (n = 40). Rosette diameter of plants in lower panel of (c). Bars indicate SD (n = 20). (e) Scanning electron micrograph of leaf epidermal cells from 20-day-old plants and comparison of leaf epidermal cell area. RNAi1-49: an RNAi line of SAUR78. nP::SAUR76/saur76: saur76 complemented with SAUR76 genomic sequence driven by native promoter. Right panel: bars indicate SD (n ≥ 20). For (d), (e) and (f), “*” and “**” indicate significant difference compared with WT at P < 0.05 and P < 0.01, respectively.
Mentions: To investigate the biological functions of SAURs in plants, SAUR76-78, driven by 35S cauliflower mosaic virus (CaMV) promoter in pROKII vector, were transformed into Arabidopsis and higher expressors were analyzed (Fig. 5a). A T-DNA insertion mutant of saur76 was also identified as a SAUR76 knockout mutant (Fig. 5b).

Bottom Line: Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity.Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2.These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

ABSTRACT
Ethylene perceived by a family of five receptors regulates many developmental processes in Arabidopsis. Here we conducted the yeast two-hybrid assay to screen for additional unidentified proteins that interact with subfamily II ethylene receptor ETR2. Three SAUR proteins, named SAUR76, 77 and 78, were identified to associate with both ETR2 and EIN4 in different assays. Interaction of SAUR76 and SAUR78 with ETR2 was further verified by co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays. Expressions of SAUR76-78 are induced by auxin and ethylene treatments. Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity. Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2. SAUR76/78 proteins are regulated by 26S proteasome system and larger tag increases their protein stability. These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

No MeSH data available.