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A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary.

Bochner F, Fellus-Alyagor L, Kalchenko V, Shinar S, Neeman M - Sci Rep (2015)

Bottom Line: Maturation of the ovarian follicle, release of the oocyte in the course of ovulation as well as formation and degradation of corpus luteum involve tightly controlled remodeling of the extracellular matrix and vasculature.Ovarian tumors, regardless of their tissue of origin, dynamically interact with the ovarian microenvironment.High-resolution dynamic imaging of such processes is particularly challenging for internal organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100 Israel.

ABSTRACT
The ovary is a dynamic organ that undergoes dramatic remodeling throughout the ovulatory cycle. Maturation of the ovarian follicle, release of the oocyte in the course of ovulation as well as formation and degradation of corpus luteum involve tightly controlled remodeling of the extracellular matrix and vasculature. Ovarian tumors, regardless of their tissue of origin, dynamically interact with the ovarian microenvironment. Their activity in the tissue encompasses recruitment of host stroma and immune cells, attachment of tumor cells to mesothelial layer, degradation of the extracellular matrix and tumor cell migration. High-resolution dynamic imaging of such processes is particularly challenging for internal organs. The implementation of a novel imaging window as reported here enabled longitudinal microscopy of ovarian physiology and orthotopic tumor invasion.

No MeSH data available.


Related in: MedlinePlus

Physiological response of the ovary to hormonal stimulation.(a–e) Stereomicroscope images and perfusion maps of the mouse ovary undergoing hormonal changes upon injection of pregnant mare’s serum gonadotropin (PMSG; injected at day 2) and human chorionic gonadotropin (hCG; injected 48 h after PMSG, at day 4). Asterix indicates the same ovarian follicle at different time points; arrow denotes the same blood vessel. Calibration bars denote color-coded intensity range (0–255) which corresponds to blood flow map of each time point. (f) Hematoxylin and eosin-stained histological section of the ovary showing corpora lutei formed after ovulation. Asterix denote corpora lutea. a-e were imaged in-vivo in the imaging window.
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f4: Physiological response of the ovary to hormonal stimulation.(a–e) Stereomicroscope images and perfusion maps of the mouse ovary undergoing hormonal changes upon injection of pregnant mare’s serum gonadotropin (PMSG; injected at day 2) and human chorionic gonadotropin (hCG; injected 48 h after PMSG, at day 4). Asterix indicates the same ovarian follicle at different time points; arrow denotes the same blood vessel. Calibration bars denote color-coded intensity range (0–255) which corresponds to blood flow map of each time point. (f) Hematoxylin and eosin-stained histological section of the ovary showing corpora lutei formed after ovulation. Asterix denote corpora lutea. a-e were imaged in-vivo in the imaging window.

Mentions: The imaging window was mounted in animals on day 0, and imaged with stereomicroscope 24 h after, on day 1 (baseline) (Fig. 4a). At this stage, the mice were sexually immature, therefore no endogenous gonadotropins were present. On day 2, they were treated with an FSH analogue, pregnant mare’s serum gonadotropin (PMSG; subcutaneous). 22 h–24 h after, on day 3, induction of follicle growth and major vascular remodeling was observed (Fig. 4b). This process continued throughout day 4, 39 h–41 h after PMSG injection (Fig. 4c). These changes are characteristic for the FSH response. 48 h after PMSG stimulation, also on day 4, the mice were administered an LH analogue, human chorionic gonadotropin (hCG; intraperitoneal). 19 hours later, on day 5, further vascular remodeling, and change of color of some of the follicles were apparent, marking the formation of corpus luteum (Fig. 4d). On day 6, approximately 43 h after hCG injection a further increase of follicle size was observed (Fig. 4e). The incidence of multiple corpora lutea, as predicted after superovulation, was confirmed with histology (Fig. 4f). Vascular blood flow maps were acquired for each time point by speckle analysis. Blood circulation in the ovary was retained throughout the whole period of the experiment.


A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary.

Bochner F, Fellus-Alyagor L, Kalchenko V, Shinar S, Neeman M - Sci Rep (2015)

Physiological response of the ovary to hormonal stimulation.(a–e) Stereomicroscope images and perfusion maps of the mouse ovary undergoing hormonal changes upon injection of pregnant mare’s serum gonadotropin (PMSG; injected at day 2) and human chorionic gonadotropin (hCG; injected 48 h after PMSG, at day 4). Asterix indicates the same ovarian follicle at different time points; arrow denotes the same blood vessel. Calibration bars denote color-coded intensity range (0–255) which corresponds to blood flow map of each time point. (f) Hematoxylin and eosin-stained histological section of the ovary showing corpora lutei formed after ovulation. Asterix denote corpora lutea. a-e were imaged in-vivo in the imaging window.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4513547&req=5

f4: Physiological response of the ovary to hormonal stimulation.(a–e) Stereomicroscope images and perfusion maps of the mouse ovary undergoing hormonal changes upon injection of pregnant mare’s serum gonadotropin (PMSG; injected at day 2) and human chorionic gonadotropin (hCG; injected 48 h after PMSG, at day 4). Asterix indicates the same ovarian follicle at different time points; arrow denotes the same blood vessel. Calibration bars denote color-coded intensity range (0–255) which corresponds to blood flow map of each time point. (f) Hematoxylin and eosin-stained histological section of the ovary showing corpora lutei formed after ovulation. Asterix denote corpora lutea. a-e were imaged in-vivo in the imaging window.
Mentions: The imaging window was mounted in animals on day 0, and imaged with stereomicroscope 24 h after, on day 1 (baseline) (Fig. 4a). At this stage, the mice were sexually immature, therefore no endogenous gonadotropins were present. On day 2, they were treated with an FSH analogue, pregnant mare’s serum gonadotropin (PMSG; subcutaneous). 22 h–24 h after, on day 3, induction of follicle growth and major vascular remodeling was observed (Fig. 4b). This process continued throughout day 4, 39 h–41 h after PMSG injection (Fig. 4c). These changes are characteristic for the FSH response. 48 h after PMSG stimulation, also on day 4, the mice were administered an LH analogue, human chorionic gonadotropin (hCG; intraperitoneal). 19 hours later, on day 5, further vascular remodeling, and change of color of some of the follicles were apparent, marking the formation of corpus luteum (Fig. 4d). On day 6, approximately 43 h after hCG injection a further increase of follicle size was observed (Fig. 4e). The incidence of multiple corpora lutea, as predicted after superovulation, was confirmed with histology (Fig. 4f). Vascular blood flow maps were acquired for each time point by speckle analysis. Blood circulation in the ovary was retained throughout the whole period of the experiment.

Bottom Line: Maturation of the ovarian follicle, release of the oocyte in the course of ovulation as well as formation and degradation of corpus luteum involve tightly controlled remodeling of the extracellular matrix and vasculature.Ovarian tumors, regardless of their tissue of origin, dynamically interact with the ovarian microenvironment.High-resolution dynamic imaging of such processes is particularly challenging for internal organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100 Israel.

ABSTRACT
The ovary is a dynamic organ that undergoes dramatic remodeling throughout the ovulatory cycle. Maturation of the ovarian follicle, release of the oocyte in the course of ovulation as well as formation and degradation of corpus luteum involve tightly controlled remodeling of the extracellular matrix and vasculature. Ovarian tumors, regardless of their tissue of origin, dynamically interact with the ovarian microenvironment. Their activity in the tissue encompasses recruitment of host stroma and immune cells, attachment of tumor cells to mesothelial layer, degradation of the extracellular matrix and tumor cell migration. High-resolution dynamic imaging of such processes is particularly challenging for internal organs. The implementation of a novel imaging window as reported here enabled longitudinal microscopy of ovarian physiology and orthotopic tumor invasion.

No MeSH data available.


Related in: MedlinePlus