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MUC-1 expression in pleomorphic adenomas using two human milk fat globule protein membrane antibodies (HMFG-1 and HMFG-2).

Ponce-Bravo S, Ledesma-Montes C, Garcés-Ortíz M - J Appl Oral Sci (2015 May-Jun)

Bottom Line: Our results showed there are two kinds of myoepithelial cells in PA.The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive.The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Odontología, Universidad Nacional Autónoma de México, México, D.F, México.

ABSTRACT

Unlabelled: Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors.

Objective: The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue.

Material and methods: Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used.

Results: Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive.

Conclusions: Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.

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Normal labial salivary gland tissue showing slight immunoexpression to HMFG-2 antibody. 400×. HMFG-2 MoAb. Avidin-biotin method
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f03: Normal labial salivary gland tissue showing slight immunoexpression to HMFG-2 antibody. 400×. HMFG-2 MoAb. Avidin-biotin method

Mentions: In labial salivary glands, immunopositivity to both MoAbs was 1+ in the ductal cells, and it varied from 1+ to 2+ in the mucous acinic cells. No immunostaining was observed in the intraductal secretion and CMyECs with both employed MoAbs (Figure 3).


MUC-1 expression in pleomorphic adenomas using two human milk fat globule protein membrane antibodies (HMFG-1 and HMFG-2).

Ponce-Bravo S, Ledesma-Montes C, Garcés-Ortíz M - J Appl Oral Sci (2015 May-Jun)

Normal labial salivary gland tissue showing slight immunoexpression to HMFG-2 antibody. 400×. HMFG-2 MoAb. Avidin-biotin method
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4510660&req=5

f03: Normal labial salivary gland tissue showing slight immunoexpression to HMFG-2 antibody. 400×. HMFG-2 MoAb. Avidin-biotin method
Mentions: In labial salivary glands, immunopositivity to both MoAbs was 1+ in the ductal cells, and it varied from 1+ to 2+ in the mucous acinic cells. No immunostaining was observed in the intraductal secretion and CMyECs with both employed MoAbs (Figure 3).

Bottom Line: Our results showed there are two kinds of myoepithelial cells in PA.The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive.The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Odontología, Universidad Nacional Autónoma de México, México, D.F, México.

ABSTRACT

Unlabelled: Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors.

Objective: The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue.

Material and methods: Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used.

Results: Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive.

Conclusions: Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.

Show MeSH
Related in: MedlinePlus