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Sodium-pump gene-expression, protein abundance and enzyme activity in isolated nephron segments of the aging rat kidney.

Scherzer P, Gal-Moscovici A, Sheikh-Hamad D, Popovtzer MM - Physiol Rep (2015)

Bottom Line: Aging is associated with alteration in renal tubular functions, including sodium handling and concentrating ability.They were higher in CCD by 110%, 115%, and 246%, respectively (P < 0.001).These results reveal quantitative changes in axial distribution of Na-K-ATPase at the level of gene expression, protein abundance, and activity in the nephrons of aging animals and may explain, in part, the pathophysiology of the senescent kidney.

View Article: PubMed Central - PubMed

Affiliation: Nephrology and Hypertension Services, Hadassah University Hospital, Jerusalem, Israel spnina@hadassah.org.il.

No MeSH data available.


Related in: MedlinePlus

Na-K-ATPase gene expression, protein abundance, and activity in MTAL from 2-, 8-, 15-, and 24-month-old rats. (A) Representative gels for α1 subunit mRNA of Na-K-ATPase and control gene (G3PDH) mRNA on ethidium bromide-stained gels. (B) Densitometry of α1 mRNA of Na-K-ATPase levels in isolated MTAL of 2-, 8-, 15-, and 24-month-old rats (shown in A). Data represent the mean and ± SEM from four independent determinations; *P < 0.05 versus 2-month-old rats. (C) Representative immunoblots show protein abundance of α1 subunit of Na-K-ATPase in the MTAL of 2, 8, 15, and 24-month- old rats. Coomassie Blue staining verified equal amounts (30 μg) of proteins loaded on the gel. (D) Data represent the mean and ±SEM from four independent determinations. (E) Na-K-ATPase activity in the MTAL from 2-, 8-, 15-, and 24-month-old rats. Na-K-ATPase activity in MTAL from 10 pairs of segments for each group at each age; *P < 0.05 versus 2 months.
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fig02: Na-K-ATPase gene expression, protein abundance, and activity in MTAL from 2-, 8-, 15-, and 24-month-old rats. (A) Representative gels for α1 subunit mRNA of Na-K-ATPase and control gene (G3PDH) mRNA on ethidium bromide-stained gels. (B) Densitometry of α1 mRNA of Na-K-ATPase levels in isolated MTAL of 2-, 8-, 15-, and 24-month-old rats (shown in A). Data represent the mean and ± SEM from four independent determinations; *P < 0.05 versus 2-month-old rats. (C) Representative immunoblots show protein abundance of α1 subunit of Na-K-ATPase in the MTAL of 2, 8, 15, and 24-month- old rats. Coomassie Blue staining verified equal amounts (30 μg) of proteins loaded on the gel. (D) Data represent the mean and ±SEM from four independent determinations. (E) Na-K-ATPase activity in the MTAL from 2-, 8-, 15-, and 24-month-old rats. Na-K-ATPase activity in MTAL from 10 pairs of segments for each group at each age; *P < 0.05 versus 2 months.

Mentions: Figure2A depicts representative gels of RT-PCR products of α1 mRNA in MTAL; Fig.2B shows densitometry of the corresponding RT-PCR products. A decrease in α1 mRNA of the enzyme by 21% and 30% in the MTAL was observed in 15- and 24- month- old rats, respectively. A fall of 59% in the α1 mRNA of Na-K-ATPase in 8-month-old rats is seen in the DCT (Table3), which persists through 24 months of age. Conversely, in CCD the α1 mRNA of Na-K-ATPase increased by 100%, 58%, and 115% in 8-, 15-, and 24-month-old rats, respectively (P < 0.001; Table3).


Sodium-pump gene-expression, protein abundance and enzyme activity in isolated nephron segments of the aging rat kidney.

Scherzer P, Gal-Moscovici A, Sheikh-Hamad D, Popovtzer MM - Physiol Rep (2015)

Na-K-ATPase gene expression, protein abundance, and activity in MTAL from 2-, 8-, 15-, and 24-month-old rats. (A) Representative gels for α1 subunit mRNA of Na-K-ATPase and control gene (G3PDH) mRNA on ethidium bromide-stained gels. (B) Densitometry of α1 mRNA of Na-K-ATPase levels in isolated MTAL of 2-, 8-, 15-, and 24-month-old rats (shown in A). Data represent the mean and ± SEM from four independent determinations; *P < 0.05 versus 2-month-old rats. (C) Representative immunoblots show protein abundance of α1 subunit of Na-K-ATPase in the MTAL of 2, 8, 15, and 24-month- old rats. Coomassie Blue staining verified equal amounts (30 μg) of proteins loaded on the gel. (D) Data represent the mean and ±SEM from four independent determinations. (E) Na-K-ATPase activity in the MTAL from 2-, 8-, 15-, and 24-month-old rats. Na-K-ATPase activity in MTAL from 10 pairs of segments for each group at each age; *P < 0.05 versus 2 months.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4510615&req=5

fig02: Na-K-ATPase gene expression, protein abundance, and activity in MTAL from 2-, 8-, 15-, and 24-month-old rats. (A) Representative gels for α1 subunit mRNA of Na-K-ATPase and control gene (G3PDH) mRNA on ethidium bromide-stained gels. (B) Densitometry of α1 mRNA of Na-K-ATPase levels in isolated MTAL of 2-, 8-, 15-, and 24-month-old rats (shown in A). Data represent the mean and ± SEM from four independent determinations; *P < 0.05 versus 2-month-old rats. (C) Representative immunoblots show protein abundance of α1 subunit of Na-K-ATPase in the MTAL of 2, 8, 15, and 24-month- old rats. Coomassie Blue staining verified equal amounts (30 μg) of proteins loaded on the gel. (D) Data represent the mean and ±SEM from four independent determinations. (E) Na-K-ATPase activity in the MTAL from 2-, 8-, 15-, and 24-month-old rats. Na-K-ATPase activity in MTAL from 10 pairs of segments for each group at each age; *P < 0.05 versus 2 months.
Mentions: Figure2A depicts representative gels of RT-PCR products of α1 mRNA in MTAL; Fig.2B shows densitometry of the corresponding RT-PCR products. A decrease in α1 mRNA of the enzyme by 21% and 30% in the MTAL was observed in 15- and 24- month- old rats, respectively. A fall of 59% in the α1 mRNA of Na-K-ATPase in 8-month-old rats is seen in the DCT (Table3), which persists through 24 months of age. Conversely, in CCD the α1 mRNA of Na-K-ATPase increased by 100%, 58%, and 115% in 8-, 15-, and 24-month-old rats, respectively (P < 0.001; Table3).

Bottom Line: Aging is associated with alteration in renal tubular functions, including sodium handling and concentrating ability.They were higher in CCD by 110%, 115%, and 246%, respectively (P < 0.001).These results reveal quantitative changes in axial distribution of Na-K-ATPase at the level of gene expression, protein abundance, and activity in the nephrons of aging animals and may explain, in part, the pathophysiology of the senescent kidney.

View Article: PubMed Central - PubMed

Affiliation: Nephrology and Hypertension Services, Hadassah University Hospital, Jerusalem, Israel spnina@hadassah.org.il.

No MeSH data available.


Related in: MedlinePlus